Abstract:
:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Markoulaki S,Hanna J,Beard C,Carey BW,Cheng AW,Lengner CJ,Dausman JA,Fu D,Gao Q,Wu S,Cassady JP,Jaenisch Rdoi
10.1038/nbt.1520subject
Has Abstractpub_date
2009-02-01 00:00:00pages
169-71issue
2eissn
1087-0156issn
1546-1696pii
nbt.1520journal_volume
27pub_type
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