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Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein.

Abstract：

:To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine beta- and kappa-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resulted in the production of 11 transgenic calves. The analysis of hormonally induced milk showed substantial expression and secretion of the transgene-derived caseins into milk. Nine cows, representing two high-expressing lines, produced milk with an 8-20% increase in beta-casein, a twofold increase in kappa-casein levels, and a markedly altered kappa-casein to total casein ratio. These results show that it is feasible to substantially alter a major component of milk in high producing dairy cows by a transgenic approach and thus to improve the functional properties of dairy milk.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Brophy B,Smolenski G,Wheeler T,Wells D,L'Huillier P,Laible G

doi

10.1038/nbt783

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

157-62

issue

2

eissn

1087-0156

issn

1546-1696

pii

nbt783

journal_volume

21

pub_type

杂志文章

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