The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K.

abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

journal_title：Nature biotechnology

authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

更新日期：2000-10-01 00:00:00

abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...

journal_title：Nature biotechnology

authors： Schöpke C,Taylor N,Cárcamo R,Konan NK,Marmey P,Henshaw GG,Beachy RN,Fauquet C

更新日期：1996-06-01 00:00:00

abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...

journal_title：Nature biotechnology

authors： Xu RH,Chen X,Li DS,Li R,Addicks GC,Glennon C,Zwaka TP,Thomson JA

更新日期：2002-12-01 00:00:00

abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...

journal_title：Nature biotechnology

authors： Lee D,Hyun JH,Jung K,Hannan P,Kwon HB

更新日期：2017-09-01 00:00:00

abstract：:Nucleotide variants, especially those related to epigenetic functions, provide critical regulatory information beyond simple genomic sequence, and they define cell status in higher organisms. 5-Methylcytosine, which is found in DNA, was until recently the only nucleotide variant studied in terms of epigenetics in euka...

journal_title：Nature biotechnology

authors： Song CX,Yi C,He C

更新日期：2012-11-01 00:00:00

abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...

journal_title：Nature biotechnology

authors： Deng J,Shoemaker R,Xie B,Gore A,LeProust EM,Antosiewicz-Bourget J,Egli D,Maherali N,Park IH,Yu J,Daley GQ,Eggan K,Hochedlinger K,Thomson J,Wang W,Gao Y,Zhang K

更新日期：2009-04-01 00:00:00

abstract：:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the...

journal_title：Nature biotechnology

authors： Kobayashi M,Shimizu S

更新日期：1998-08-01 00:00:00

abstract：:Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...

journal_title：Nature biotechnology

authors： Eppich HM,Foxall R,Gaynor K,Dombkowski D,Miura N,Cheng T,Silva-Arrieta S,Evans RH,Mangano JA,Preffer FI,Scadden DT

更新日期：2000-08-01 00:00:00

abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...

journal_title：Nature biotechnology

authors： Wang SJ

更新日期：2008-05-01 00:00:00

abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...

journal_title：Nature biotechnology

authors： Kalhor R,Tjong H,Jayathilaka N,Alber F,Chen L

更新日期：2011-12-25 00:00:00

abstract：:Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...

journal_title：Nature biotechnology

authors： Yang XW,Model P,Heintz N

更新日期：1997-09-01 00:00:00

abstract：:A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....

journal_title：Nature biotechnology

authors： Zhang Z,Bai L,Cong L,Yu P,Zhang T,Shi W,Li F,Du J,Wang K

更新日期：2021-01-01 00:00:00

abstract：:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...

journal_title：Nature biotechnology

authors： Haasnoot J,Westerhout EM,Berkhout B

更新日期：2007-12-01 00:00:00

abstract：:Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

journal_title：Nature biotechnology

authors： Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

更新日期：2018-11-19 00:00:00

abstract：:Epigenetic modifications constitute a complex regulatory layer on top of the genome sequence. Pluripotent and differentiated cells provide a powerful system for investigating how the epigenetic code influences cellular fate. High-throughput sequencing of these cell types has yielded DNA methylation maps at single-nucl...

journal_title：Nature biotechnology

authors： Meissner A

更新日期：2010-10-01 00:00:00

abstract：:Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...

journal_title：Nature biotechnology

authors： Garriga E,Di Tommaso P,Magis C,Erb I,Mansouri L,Baltzis A,Laayouni H,Kondrashov F,Floden E,Notredame C

更新日期：2019-12-01 00:00:00

abstract：:Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...

journal_title：Nature biotechnology

authors： Zhang G,Liu X,Quan Z,Cheng S,Xu X,Pan S,Xie M,Zeng P,Yue Z,Wang W,Tao Y,Bian C,Han C,Xia Q,Peng X,Cao R,Yang X,Zhan D,Hu J,Zhang Y,Li H,Li H,Li N,Wang J,Wang C,Wang R,Guo T,Cai Y,Liu C,Xiang H,

更新日期：2012-05-13 00:00:00

abstract：:Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common...

journal_title：Nature biotechnology

authors： Doxey AC,Yaish MW,Griffith M,McConkey BJ

更新日期：2006-07-01 00:00:00

abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...

journal_title：Nature biotechnology

authors： Oger P,Petit A,Dessaux Y

更新日期：1997-04-01 00:00:00

abstract：:CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...

journal_title：Nature biotechnology

authors： Doench JG,Fusi N,Sullender M,Hegde M,Vaimberg EW,Donovan KF,Smith I,Tothova Z,Wilen C,Orchard R,Virgin HW,Listgarten J,Root DE

更新日期：2016-02-01 00:00:00

abstract：:Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...

journal_title：Nature biotechnology

authors： Glenz K,Bouchon B,Stehle T,Wallich R,Simon MM,Warzecha H

更新日期：2006-01-01 00:00:00

abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...

journal_title：Nature biotechnology

authors： Hilton IB,D'Ippolito AM,Vockley CM,Thakore PI,Crawford GE,Reddy TE,Gersbach CA

更新日期：2015-05-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Lam AT,Ma J,Barr C,Lee SA,White AK,Yu K,Riedel-Kruse IH

更新日期：2019-12-01 00:00:00

abstract：:The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...

journal_title：Nature biotechnology

authors： Bargehr J,Ong LP,Colzani M,Davaapil H,Hofsteen P,Bhandari S,Gambardella L,Le Novère N,Iyer D,Sampaziotis F,Weinberger F,Bertero A,Leonard A,Bernard WG,Martinson A,Figg N,Regnier M,Bennett MR,Murry CE,Sinha S

更新日期：2019-08-01 00:00:00

abstract：:Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient exp...

journal_title：Nature biotechnology

authors： Mercer TR,Gerhardt DJ,Dinger ME,Crawford J,Trapnell C,Jeddeloh JA,Mattick JS,Rinn JL

更新日期：2011-11-13 00:00:00

abstract：:Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators). When replicators were included in si...

journal_title：Nature biotechnology

authors： Fu H,Wang L,Lin CM,Singhania S,Bouhassira EE,Aladjem MI

更新日期：2006-05-01 00:00:00

abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...

journal_title：Nature biotechnology

authors： Segal E,Sirlin CB,Ooi C,Adler AS,Gollub J,Chen X,Chan BK,Matcuk GR,Barry CT,Chang HY,Kuo MD

更新日期：2007-06-01 00:00:00

abstract：:New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...

journal_title：Nature biotechnology

authors： Sakamoto T,Morinaka Y,Ohnishi T,Sunohara H,Fujioka S,Ueguchi-Tanaka M,Mizutani M,Sakata K,Takatsuto S,Yoshida S,Tanaka H,Kitano H,Matsuoka M

更新日期：2006-01-01 00:00:00

abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...

journal_title：Nature biotechnology

authors： Myers JW,Jones JT,Meyer T,Ferrell JE Jr

更新日期：2003-03-01 00:00:00

abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...

journal_title：Nature biotechnology

authors： DiCarlo JE,Chavez A,Dietz SL,Esvelt KM,Church GM

更新日期：2015-12-01 00:00:00