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Mapping recently identified nucleotide variants in the genome and transcriptome.

Abstract：

:Nucleotide variants, especially those related to epigenetic functions, provide critical regulatory information beyond simple genomic sequence, and they define cell status in higher organisms. 5-Methylcytosine, which is found in DNA, was until recently the only nucleotide variant studied in terms of epigenetics in eukaryotes. However, 5-methylcytosine has turned out to be just one component of a dynamic DNA epigenetic regulatory network that also includes 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine. Recently, reversible methylation of N6-methyladenosine in RNA has also been demonstrated. The discovery of these new nucleotide variants triggered an explosion of new information in the epigenetics field. This rapid research progress has benefited significantly from timely developments of new technologies that specifically recognize, enrich and sequence nucleotide modifications, as evidenced by the wide application of the bisulfite sequencing of 5-methylcytosine and very recent modifications of bisulfite sequencing to resolve 5-hydroxymethylcytosine from 5-methylcytosine with base-resolution information.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Song CX,Yi C,He C

doi

10.1038/nbt.2398

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

1107-16

issue

11

eissn

1087-0156

issn

1546-1696

pii

nbt.2398

journal_volume

30

pub_type

杂志文章,评审

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