Abstract：
:Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient expression is below the detection limits of conventional sequencing approaches. We use the unprecedented depth of coverage afforded by this technique to reach the deepest limits of the human transcriptome, exposing widespread, regulated and remarkably complex noncoding transcription in intergenic regions, as well as unannotated exons and splicing patterns in even intensively studied protein-coding loci such as p53 and HOX. The data also show that intermittent sequenced reads observed in conventional RNA sequencing data sets, previously dismissed as noise, are in fact indicative of unassembled rare transcripts. Collectively, these results reveal the range, depth and complexity of a human transcriptome that is far from fully characterized.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Mercer TR,Gerhardt DJ,Dinger ME,Crawford J,Trapnell C,Jeddeloh JA,Mattick JS,Rinn JLdoi
10.1038/nbt.2024subject
Has Abstractpub_date
2011-11-13 00:00:00pages
99-104issue
1eissn
1087-0156issn
1546-1696pii
nbt.2024journal_volume
30pub_type
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