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Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase.

Abstract：

:Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achieving enzymatic activity on the surface. More than 80% of the OPH activity was located on the cell surface as determined by protease accessibility experiments. Whole cells expressing OPH on the cell surface degraded parathion and paraoxon very effectively without any diffusional limitation, resulting in sevenfold higher rates of parathion degradation compared with whole cells with similar levels of intracellular OPH. Immobilization of these live biocatalysts onto solid supports could provide an attractive means for pesticide detoxification in place of immobilized enzymes, affording a reduced diffusional barrier.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Richins RD,Kaneva I,Mulchandani A,Chen W

doi

10.1038/nbt1097-984

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

984-7

issue

10

eissn

1087-0156

issn

1546-1696

journal_volume

15

pub_type

杂志文章

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