Short hairpin RNA-expressing bacteria elicit RNA interference in mammals.

abstract：:Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...

journal_title：Nature biotechnology

authors： Richins RD,Kaneva I,Mulchandani A,Chen W

更新日期：1997-10-01 00:00:00

abstract：:We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...

journal_title：Nature biotechnology

authors： Majander K,Anton L,Antikainen J,Lång H,Brummer M,Korhonen TK,Westerlund-Wikström B

更新日期：2005-04-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,

更新日期：2020-08-01 00:00:00

abstract：:Pluripotent stem cell-derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal m...

journal_title：Nature biotechnology

authors： Liu YW,Chen B,Yang X,Fugate JA,Kalucki FA,Futakuchi-Tsuchida A,Couture L,Vogel KW,Astley CA,Baldessari A,Ogle J,Don CW,Steinberg ZL,Seslar SP,Tuck SA,Tsuchida H,Naumova AV,Dupras SK,Lyu MS,Lee J,Hailey DW,Reinec

更新日期：2018-08-01 00:00:00

abstract：:Industrial strain development requires system-wide engineering and optimization of cellular metabolism while considering industrially relevant fermentation and recovery processes. It can be conceptualized as several strategies, which may be implemented in an iterative fashion and in different orders. The key challenge...

journal_title：Nature biotechnology

authors： Lee SY,Kim HU

更新日期：2015-10-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:In the version of this article initially published, Lena Dolman's second affiliation was given as Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK. The correct second affiliation is Ontario Institute for Cancer Research, Toronto, Ontario, Canada. The error has been corrected in the HTML ...

journal_title：Nature biotechnology

authors： Fiume M,Cupak M,Keenan S,Rambla J,de la Torre S,Dyke SOM,Brookes AJ,Carey K,Lloyd D,Goodhand P,Haeussler M,Baudis M,Stockinger H,Dolman L,Lappalainen I,Törnroos J,Linden M,Spalding JD,Ur-Rehman S,Page A,Flicek P,

更新日期：2019-04-01 00:00:00

abstract：:The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines into a therapeutic HER2/neu antibody at three sites differing in solvent accessibility and local charge. The highly solvent-accessible si...

journal_title：Nature biotechnology

authors： Shen BQ,Xu K,Liu L,Raab H,Bhakta S,Kenrick M,Parsons-Reponte KL,Tien J,Yu SF,Mai E,Li D,Tibbitts J,Baudys J,Saad OM,Scales SJ,McDonald PJ,Hass PE,Eigenbrot C,Nguyen T,Solis WA,Fuji RN,Flagella KM,Patel D,Spe

更新日期：2012-01-22 00:00:00

abstract：:For the past five decades, the need for new antibiotics has been met largely by semisynthetic tailoring of natural product scaffolds discovered in the middle of the 20(th) century. More recently, however, advances in technology have sparked a resurgence in the discovery of natural product antibiotics from bacterial so...

journal_title：Nature biotechnology

authors： Clardy J,Fischbach MA,Walsh CT

更新日期：2006-12-01 00:00:00

abstract：:Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators). When replicators were included in si...

journal_title：Nature biotechnology

authors： Fu H,Wang L,Lin CM,Singhania S,Bouhassira EE,Aladjem MI

更新日期：2006-05-01 00:00:00

abstract：:We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...

journal_title：Nature biotechnology

authors： Kim K,Zhao R,Doi A,Ng K,Unternaehrer J,Cahan P,Huo H,Loh YH,Aryee MJ,Lensch MW,Li H,Collins JJ,Feinberg AP,Daley GQ

更新日期：2011-11-27 00:00:00

abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

journal_title：Nature biotechnology

authors： Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

更新日期：2018-02-01 00:00:00

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...

journal_title：Nature biotechnology

authors： Deverman BE,Pravdo PL,Simpson BP,Kumar SR,Chan KY,Banerjee A,Wu WL,Yang B,Huber N,Pasca SP,Gradinaru V

更新日期：2016-02-01 00:00:00

abstract：:In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. ...

journal_title：Nature biotechnology

authors： Dolgin E

更新日期：2019-02-01 00:00:00

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million to...

journal_title：Nature biotechnology

authors： López-Bucio J,de La Vega OM,Guevara-García A,Herrera-Estrella L

更新日期：2000-04-01 00:00:00

abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...

journal_title：Nature biotechnology

authors： Kleifeld O,Doucet A,auf dem Keller U,Prudova A,Schilling O,Kainthan RK,Starr AE,Foster LJ,Kizhakkedathu JN,Overall CM

更新日期：2010-03-01 00:00:00

abstract：:To select for bacterial strains with enhanced phenotypes, random fragments of a whole genome, or a defined region of the genome, are cloned in a nonreplicating vector. The resulting plasmids are integrated by recombination into the homologous DNA region of the original strain. Integration gives rise to a nontandem dir...

journal_title：Nature biotechnology

authors： Mavingui P,Flores M,Romero D,Martínez-Romero E,Palacios R

更新日期：1997-06-01 00:00:00

abstract：:The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes compr...

journal_title：Nature biotechnology

authors： Berger I,Fitzgerald DJ,Richmond TJ

更新日期：2004-12-01 00:00:00

abstract：:Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...

journal_title：Nature biotechnology

authors： Superti-Furga G,Jönsson K,Courtneidge SA

更新日期：1996-05-01 00:00:00

abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...

journal_title：Nature biotechnology

authors： Barennes P,Quiniou V,Shugay M,Egorov ES,Davydov AN,Chudakov DM,Uddin I,Ismail M,Oakes T,Chain B,Eugster A,Kashofer K,Rainer PP,Darko S,Ransier A,Douek DC,Klatzmann D,Mariotti-Ferrandiz E

更新日期：2020-09-07 00:00:00

abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...

journal_title：Nature biotechnology

authors： Zhu Q,Shah S,Dries R,Cai L,Yuan GC

更新日期：2018-10-29 00:00:00

abstract：:Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...

journal_title：Nature biotechnology

authors： Chen R,Shi L,Hakenberg J,Naughton B,Sklar P,Zhang J,Zhou H,Tian L,Prakash O,Lemire M,Sleiman P,Cheng WY,Chen W,Shah H,Shen Y,Fromer M,Omberg L,Deardorff MA,Zackai E,Bobe JR,Levin E,Hudson TJ,Groop L,Wang J,

更新日期：2016-05-01 00:00:00

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly, we present Shasta, a de novo long-read assembler, and polishing algorithms named MarginPolish and HELEN. Using a single P...

journal_title：Nature biotechnology

authors： Shafin K,Pesout T,Lorig-Roach R,Haukness M,Olsen HE,Bosworth C,Armstrong J,Tigyi K,Maurer N,Koren S,Sedlazeck FJ,Marschall T,Mayes S,Costa V,Zook JM,Liu KJ,Kilburn D,Sorensen M,Munson KM,Vollger MR,Monlong J,Gar

更新日期：2020-09-01 00:00:00

abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...

journal_title：Nature biotechnology

authors： Isaacs FJ,Dwyer DJ,Ding C,Pervouchine DD,Cantor CR,Collins JJ

更新日期：2004-07-01 00:00:00

abstract：:The human transcriptome is marked by extensive alternative mRNA splicing and the expression of many closely related genes, which may be difficult to distinguish using standard microarray techniques. Here we describe a sensitive and specific assay for parallel analysis of mRNA isoforms on a fiber-optic microarray platf...

journal_title：Nature biotechnology

authors： Yeakley JM,Fan JB,Doucet D,Luo L,Wickham E,Ye Z,Chee MS,Fu XD

更新日期：2002-04-01 00:00:00

abstract：:Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...

journal_title：Nature biotechnology

authors： Dove A

更新日期：2000-10-01 00:00:00

abstract：:A problem in analyzing microarray-based gene expression data is the separation of genes causally involved in a disease from innocent bystander genes, whose expression levels have been secondarily altered by primary changes elsewhere. To investigate this issue systematically in the context of a class of complex human d...

journal_title：Nature biotechnology

authors： Miklos GL,Maleszka R

更新日期：2004-05-01 00:00:00