Abstract:
:An amendment to this paper has been published and can be accessed via a link at the top of the paper.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,doi
10.1038/s41587-020-0578-0subject
Has Abstractpub_date
2020-08-01 00:00:00pages
1001issue
8eissn
1087-0156issn
1546-1696pii
10.1038/s41587-020-0578-0journal_volume
38pub_type
已发布勘误abstract::The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approximately 146 bp is wrapped around a core of 8 histone molecules. Post-translational histone modifications play an essential role in modifying chromatin structure. Here we ap...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt990
更新日期:2004-08-01 00:00:00
abstract::Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1588
更新日期:2009-12-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0510-7
更新日期:2020-05-01 00:00:00
abstract::Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, rob...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1629
更新日期:2010-06-01 00:00:00
abstract::Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural' natural products but existing techniques for this are time consuming...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1128
更新日期:2005-09-01 00:00:00
abstract::Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0344-3
更新日期:2020-03-01 00:00:00
abstract::Despite developments in targeted gene sequencing and whole-genome analysis techniques, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Here we present targeted locus amplification (TLA), a strategy to se...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2959
更新日期:2014-10-01 00:00:00
abstract::We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/76469
更新日期:2000-06-01 00:00:00
abstract::Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0702-729
更新日期:2002-07-01 00:00:00
abstract::Targeting of secreted and cell-surface proteins to the cell membrane is mediated by a short hydrophobic stretch of amino acids, termed the signal sequence. We have developed a method that detects signal sequences in cDNA fragments based on their ability to redirect a constitutively active mutant of a cytokine receptor...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/8666
更新日期:1999-05-01 00:00:00
abstract::Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencin...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1914
更新日期:2011-07-31 00:00:00
abstract::Bone morphogenetic proteins (BMPs) that have the potential to elicit new bone in vivo have been used in a tissue-engineering approach for the repair of bone injuries and bone defects. Although it is now possible to generate large amounts of recombinant human (rh) BMPs for medical use, the major challenge remains in th...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/86715
更新日期:2001-04-01 00:00:00
abstract::We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2057
更新日期:2011-12-25 00:00:00
abstract::We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3353
更新日期:2015-10-01 00:00:00
abstract::Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3267
更新日期:2015-07-01 00:00:00
abstract::New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1173
更新日期:2006-01-01 00:00:00
abstract::To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine beta- and kappa-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resul...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt783
更新日期:2003-02-01 00:00:00
abstract::Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...
journal_title:Nature biotechnology
pub_type:
doi:10.1038/nbt726
更新日期:2002-09-01 00:00:00
abstract::The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines into a therapeutic HER2/neu antibody at three sites differing in solvent accessibility and local charge. The highly solvent-accessible si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2108
更新日期:2012-01-22 00:00:00
abstract::Rice is a staple crop that has undergone substantial phenotypic and physiological changes during domestication. Here we resequenced the genomes of 40 cultivated accessions selected from the major groups of rice and 10 accessions of their wild progenitors (Oryza rufipogon and Oryza nivara) to >15 × raw data coverage. W...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2050
更新日期:2011-12-11 00:00:00
abstract::We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, acti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/13732
更新日期:1999-10-01 00:00:00
abstract::Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3247
更新日期:2015-07-01 00:00:00
abstract::An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded af...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1001-946
更新日期:2001-10-01 00:00:00
abstract::The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1632
更新日期:2010-05-01 00:00:00
abstract::The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt0198-33
更新日期:1998-01-01 00:00:00
abstract::Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt0207-185
更新日期:2007-02-01 00:00:00
abstract::In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1335
更新日期:2007-10-01 00:00:00
abstract::A fibrinolytic agent consisting of a tissue-type plasminogen activator (tPA) coupled to the surface of red blood cells (RBCs) can dissolve nascent clots from within the clot, in a Trojan horse-like strategy, while having minimal effects on preexisting hemostatic clots or extravascular tissue. After intravenous injecti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt846
更新日期:2003-08-01 00:00:00
abstract::We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2052
更新日期:2011-11-27 00:00:00
abstract::Whole-genome mRNA quantitation can be used to identify the genes that are most responsive to environmental or genotypic change. By searching for mutually similar DNA elements among the upstream non-coding DNA sequences of these genes, we can identify candidate regulatory motifs and corresponding candidate sets of core...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1098-939
更新日期:1998-10-01 00:00:00