Abstract：
:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay between many genes and the environment. These investigations will require large numbers of DNA markers and the technology to screen large populations with these markers. The systematic identification of the common DNA polymorphisms in the human genome coupled with the development of high throughput screening methods should allow ultimately the elucidation of the genetic component of most clinical and nonclinical phenotypes.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Schafer AJ,Hawkins JRdoi
10.1038/nbt0198-33subject
Has Abstractpub_date
1998-01-01 00:00:00pages
33-9issue
1eissn
1087-0156issn
1546-1696journal_volume
16pub_type
杂志文章,评审abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1404
更新日期：2008-05-01 00:00:00
abstract：:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/11765
更新日期：1999-08-01 00:00:00
abstract：:The exocrine pancreas, liver, and submandibular glands of the rat were used to express and secrete two exogenous, human protein hormones (growth hormone and insulin) into blood at physiological concentrations. Transfection, expression, and secretion were achieved by the in vivo retrograde injection of plasmid DNA into...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1297-1378
更新日期：1997-12-01 00:00:00
abstract：:The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt872
更新日期：2003-10-01 00:00:00
abstract：:Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0398-281
更新日期：1998-03-01 00:00:00
abstract：:Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1196-1557
更新日期：1996-11-01 00:00:00
abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1110-1157
更新日期：2010-11-01 00:00:00
abstract：:The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1632
更新日期：2010-05-01 00:00:00
abstract：:Whole-genome mRNA quantitation can be used to identify the genes that are most responsive to environmental or genotypic change. By searching for mutually similar DNA elements among the upstream non-coding DNA sequences of these genes, we can identify candidate regulatory motifs and corresponding candidate sets of core...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1098-939
更新日期：1998-10-01 00:00:00
abstract：:Industrial strain development requires system-wide engineering and optimization of cellular metabolism while considering industrially relevant fermentation and recovery processes. It can be conceptualized as several strategies, which may be implemented in an iterative fashion and in different orders. The key challenge...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.3365
更新日期：2015-10-01 00:00:00
abstract：:DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0302-282
更新日期：2002-03-01 00:00:00
abstract：:DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/90816
更新日期：2001-08-01 00:00:00
abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.3765
更新日期：2017-03-01 00:00:00
abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3412
更新日期：2015-12-01 00:00:00
abstract：:The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1641
更新日期：2010-06-01 00:00:00
abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0208-183
更新日期：2008-02-01 00:00:00
abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2862
更新日期：2014-05-01 00:00:00
abstract：:Functional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates. A central challenge is the definition of the most appropriate design criteria, which will benefit cr...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1064
更新日期：2005-02-01 00:00:00
abstract：:In most tree-breeding programs worldwide, increasing the trees' growth rates and stem volumes and shortening their rotation times are important aims. Such trees would yield more biomass per unit area. Here we show that overexpressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hy...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/77355
更新日期：2000-07-01 00:00:00
abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2427
更新日期：2012-12-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0253-5
更新日期：2019-09-01 00:00:00
abstract：:A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1145
更新日期：2005-10-01 00:00:00
abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
abstract：:The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1197-1271
更新日期：1997-11-01 00:00:00
abstract：:We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/10883
更新日期：1999-07-01 00:00:00
abstract：:Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2689
更新日期：2013-11-01 00:00:00
abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/76469
更新日期：2000-06-01 00:00:00
abstract：:The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0412-8
更新日期：2020-04-01 00:00:00
abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/84422
更新日期：2001-02-01 00:00:00
abstract：:Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt726
更新日期：2002-09-01 00:00:00
