Abstract:
:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the ribosome binding site in a target gene. Upon transcription, this cis-repressive sequence causes a stem-loop structure to form at the 5'-untranslated region of the mRNA. The stem-loop structure interferes with ribosome binding, silencing gene expression. A small noncoding RNA that is expressed in trans targets the cis-repressed RNA with high specificity, causing an alteration in the stem-loop structure that activates expression. Such engineered riboregulators may lend insight into mechanistic actions of endogenous RNA-based processes and could serve as scalable components of biological networks, able to function with any promoter or gene to directly control gene expression.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Isaacs FJ,Dwyer DJ,Ding C,Pervouchine DD,Cantor CR,Collins JJdoi
10.1038/nbt986subject
Has Abstractpub_date
2004-07-01 00:00:00pages
841-7issue
7eissn
1087-0156issn
1546-1696pii
nbt986journal_volume
22pub_type
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