Molecular beacons: probes that fluoresce upon hybridization.

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:We show that transplantation of adult bone marrow-derived cells expressing c-kit reduces hyperglycemia in mice with streptozotocin-induced pancreatic damage. Although quantitative analysis of the pancreas revealed a low frequency of donor insulin-positive cells, these cells were not present at the onset of blood gluco...

journal_title：Nature biotechnology

authors： Hess D,Li L,Martin M,Sakano S,Hill D,Strutt B,Thyssen S,Gray DA,Bhatia M

更新日期：2003-07-01 00:00:00

abstract：:Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and ...

journal_title：Nature biotechnology

authors： Barrangou R,Doudna JA

更新日期：2016-01-01 00:00:00

abstract：:Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...

journal_title：Nature biotechnology

authors： Jeck WR,Sharpless NE

更新日期：2014-05-01 00:00:00

abstract：:Recent therapeutic successes have renewed interest in drug combinations, but experimental screening approaches are costly and often identify only small numbers of synergistic combinations. The DREAM consortium launched an open challenge to foster the development of in silico methods to computationally rank 91 compound...

journal_title：Nature biotechnology

authors： Bansal M,Yang J,Karan C,Menden MP,Costello JC,Tang H,Xiao G,Li Y,Allen J,Zhong R,Chen B,Kim M,Wang T,Heiser LM,Realubit R,Mattioli M,Alvarez MJ,Shen Y,NCI-DREAM Community.,Gallahan D,Singer D,Saez-Rodriguez J,

更新日期：2014-12-01 00:00:00

abstract：:Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...

journal_title：Nature biotechnology

authors： Gat-Viks I,Chevrier N,Wilentzik R,Eisenhaure T,Raychowdhury R,Steuerman Y,Shalek AK,Hacohen N,Amit I,Regev A

更新日期：2013-04-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,

更新日期：2020-08-01 00:00:00

abstract：:Delivery of short interfering RNAs (siRNAs) remains a key challenge in the development of RNA interference (RNAi) therapeutics. A better understanding of the mechanisms of siRNA cellular uptake, intracellular transport and endosomal release could critically contribute to the improvement of delivery methods. Here we mo...

journal_title：Nature biotechnology

authors： Gilleron J,Querbes W,Zeigerer A,Borodovsky A,Marsico G,Schubert U,Manygoats K,Seifert S,Andree C,Stöter M,Epstein-Barash H,Zhang L,Koteliansky V,Fitzgerald K,Fava E,Bickle M,Kalaidzidis Y,Akinc A,Maier M,Zerial M

更新日期：2013-07-01 00:00:00

abstract：:The nonprofit Pew Initiative on Food and Biotechnology is closing, but the need for an independent and neutral body to facilitate dialog on US biotech policy has never been greater. ...

journal_title：Nature biotechnology

authors：

更新日期：2007-02-01 00:00:00

abstract：:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...

journal_title：Nature biotechnology

authors： Chaerle L,Van Caeneghem W,Messens E,Lambers H,Van Montagu M,Van Der Straeten D

更新日期：1999-08-01 00:00:00

abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

journal_title：Nature biotechnology

authors： Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

更新日期：2018-02-01 00:00:00

abstract：:A fibrinolytic agent consisting of a tissue-type plasminogen activator (tPA) coupled to the surface of red blood cells (RBCs) can dissolve nascent clots from within the clot, in a Trojan horse-like strategy, while having minimal effects on preexisting hemostatic clots or extravascular tissue. After intravenous injecti...

journal_title：Nature biotechnology

authors： Murciano JC,Medinilla S,Eslin D,Atochina E,Cines DB,Muzykantov VR

更新日期：2003-08-01 00:00:00

abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...

journal_title：Nature biotechnology

authors： Tibbe AG,de Grooth BG,Greve J,Liberti PA,Dolan GJ,Terstappen LW

更新日期：1999-12-01 00:00:00

abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...

journal_title：Nature biotechnology

authors： Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama IN

更新日期：1997-01-01 00:00:00

abstract：:The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...

journal_title：Nature biotechnology

authors： Whitesides GM

更新日期：2003-10-01 00:00:00

abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...

journal_title：Nature biotechnology

authors： Bothmann H,Plückthun A

更新日期：1998-04-01 00:00:00

abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...

journal_title：Nature biotechnology

authors： Lewis K,Ausubel FM

更新日期：2006-12-01 00:00:00

abstract：:Functional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates. A central challenge is the definition of the most appropriate design criteria, which will benefit cr...

journal_title：Nature biotechnology

authors： Rao BM,Lauffenburger DA,Wittrup KD

更新日期：2005-02-01 00:00:00

abstract：:Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogen...

journal_title：Nature biotechnology

authors： Li YH,Zhou G,Ma J,Jiang W,Jin LG,Zhang Z,Guo Y,Zhang J,Sui Y,Zheng L,Zhang SS,Zuo Q,Shi XH,Li YF,Zhang WK,Hu Y,Kong G,Hong HL,Tan B,Song J,Liu ZX,Wang Y,Ruan H,Yeung CK,Liu J,Wang H,Zhang LJ,Guan RX

更新日期：2014-10-01 00:00:00

abstract：:Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a ce...

journal_title：Nature biotechnology

authors： Wang RF,Wang HY

更新日期：2002-02-01 00:00:00

abstract：:DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...

journal_title：Nature biotechnology

authors： Boon EM,Salas JE,Barton JK

更新日期：2002-03-01 00:00:00

abstract：:RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...

journal_title：Nature biotechnology

authors： Eguchi A,Meade BR,Chang YC,Fredrickson CT,Willert K,Puri N,Dowdy SF

更新日期：2009-06-01 00:00:00

abstract：:DNA microarrays have the ability to analyze the expression of thousands of the same set of genes under at least two different experimental conditions. However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial tota...

journal_title：Nature biotechnology

authors： Talaat AM,Hunter P,Johnston SA

更新日期：2000-06-01 00:00:00

abstract：:The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...

journal_title：Nature biotechnology

authors： Bargehr J,Ong LP,Colzani M,Davaapil H,Hofsteen P,Bhandari S,Gambardella L,Le Novère N,Iyer D,Sampaziotis F,Weinberger F,Bertero A,Leonard A,Bernard WG,Martinson A,Figg N,Regnier M,Bennett MR,Murry CE,Sinha S

更新日期：2019-08-01 00:00:00

abstract：:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...

journal_title：Nature biotechnology

authors： Hare PD,Chua NH

更新日期：2002-06-01 00:00:00

abstract：:Structural genomics has the ambitious goal of delivering three-dimensional structural information on a genome-wide scale. Yet only a small fraction of natural proteins are suitable for structure determination because of bottlenecks such as poor expression, aggregation, and misfolding of proteins, and difficulties in s...

journal_title：Nature biotechnology

authors： Pédelacq JD,Piltch E,Liong EC,Berendzen J,Kim CY,Rho BS,Park MS,Terwilliger TC,Waldo GS

更新日期：2002-09-01 00:00:00

abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...

journal_title：Nature biotechnology

authors： Verkhusha VV,Lukyanov KA

更新日期：2004-03-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...

journal_title：Nature biotechnology

authors： Ishizaki-Nishizawa O,Fujii T,Azuma M,Sekiguchi K,Murata N,Ohtani T,Toguri T

更新日期：1996-08-01 00:00:00

abstract：:Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. Stage (S) 7 cells expressed key markers of mature pancreatic...

journal_title：Nature biotechnology

authors： Rezania A,Bruin JE,Arora P,Rubin A,Batushansky I,Asadi A,O'Dwyer S,Quiskamp N,Mojibian M,Albrecht T,Yang YH,Johnson JD,Kieffer TJ

更新日期：2014-11-01 00:00:00