Abstract:
:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome after they have fulfilled their purpose. Recently, chemically inducible site-specific recombinase systems have emerged as valuable tools for efficiently regulating the excision of transgenes when their expression is no longer required. The implementation of these strategies in crops and their further improvement will help to expedite widespread public acceptance of agricultural biotechnology
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Hare PD,Chua NHdoi
10.1038/nbt0602-575subject
Has Abstractpub_date
2002-06-01 00:00:00pages
575-80issue
6eissn
1087-0156issn
1546-1696pii
nbt0602-575journal_volume
20pub_type
杂志文章,评审abstract::A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1145
更新日期:2005-10-01 00:00:00
abstract::We used the integrase from phage phiC31 to integrate the human Factor IX (hFIX) gene permanently into specific sites in the mouse genome. A plasmid containing attB and an expression cassette for hFIX was delivered to the livers of mice by using high-pressure tail vein injection. When an integrase expression plasmid wa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt753
更新日期:2002-11-01 00:00:00
abstract::The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1162
更新日期:2005-12-01 00:00:00
abstract::We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/85712
更新日期:2001-03-01 00:00:00
abstract::Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0054-x
更新日期:2019-05-01 00:00:00
abstract::RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...
journal_title:Nature biotechnology
pub_type:
doi:10.1038/nbt792
更新日期:2003-03-01 00:00:00
abstract::St. Louis wants to become a hub of agricultural biotechnology. All it needs is more start-ups and funds. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0408-471
更新日期:2008-04-01 00:00:00
abstract::Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4047
更新日期:2018-02-01 00:00:00
abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt853
更新日期:2003-09-01 00:00:00
abstract::Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3440
更新日期:2016-02-01 00:00:00
abstract::Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0293-x
更新日期:2019-12-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0510-7
更新日期:2020-05-01 00:00:00
abstract::We describe a new method of random mutagenesis that employs the addition of peptide tails with random sequences to the C-terminal of enzyme molecules. A mutant population of catalase I from Bacillus stearothermophilus prepared by this method has a diversity in thermostability and enzyme activity equal to that obtained...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/5232
更新日期:1999-01-01 00:00:00
abstract::A major challenge for fluorescence imaging of living mammalian cells is maintaining viability following prolonged exposure to excitation illumination. We have monitored the dynamics of mitochondrial distribution in hamster embryos at frequent intervals over 24 h using two-photon microscopy (1,047 nm) while maintaining...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/11698
更新日期:1999-08-01 00:00:00
abstract::Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1076
更新日期:2005-04-01 00:00:00
abstract::Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3837
更新日期:2017-06-01 00:00:00
abstract::Efficient delivery of small interfering (si)RNA to specific cell populations in vivo remains a formidable challenge to its successful therapeutic application. We show that siRNA synthetically linked to a CpG oligonucleotide agonist of toll-like receptor (TLR)9 targets and silences genes in TLR9(+) myeloid cells and B ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1564
更新日期:2009-10-01 00:00:00
abstract::Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase transition occurs, leading to aggregation of the polypeptide. We demonstrate a method for purification of soluble fusio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/15100
更新日期:1999-11-01 00:00:00
abstract::The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt802
更新日期:2003-04-01 00:00:00
abstract::Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0606-643
更新日期:2006-06-01 00:00:00
abstract::Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2195
更新日期:2012-05-13 00:00:00
abstract::The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines into a therapeutic HER2/neu antibody at three sites differing in solvent accessibility and local charge. The highly solvent-accessible si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2108
更新日期:2012-01-22 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0578-0
更新日期:2020-08-01 00:00:00
abstract::Engineered T cells are effective therapies against a range of malignancies, but current approaches rely on autologous T cells, which are difficult and expensive to manufacture. Efforts to develop potent allogeneic T cells that are not rejected by the recipient's immune system require abrogating both T- and natural kil...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0601-5
更新日期:2021-01-01 00:00:00
abstract::Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/83489
更新日期:2001-01-01 00:00:00
abstract::Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2689
更新日期:2013-11-01 00:00:00
abstract::Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1275
更新日期:2007-01-01 00:00:00
abstract::DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/90816
更新日期:2001-08-01 00:00:00
abstract::Whole-genome mRNA quantitation can be used to identify the genes that are most responsive to environmental or genotypic change. By searching for mutually similar DNA elements among the upstream non-coding DNA sequences of these genes, we can identify candidate regulatory motifs and corresponding candidate sets of core...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1098-939
更新日期:1998-10-01 00:00:00
abstract::An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0508-519
更新日期:2008-05-01 00:00:00