Abstract：
:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DRdoi
10.1038/nbt.4052subject
Has Abstractpub_date
2018-02-01 00:00:00pages
152-155issue
2eissn
1087-0156issn
1546-1696pii
nbt.4052journal_volume
36pub_type
杂志文章abstract：:Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural' natural products but existing techniques for this are time consuming...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1128
更新日期：2005-09-01 00:00:00
abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1559
更新日期：2009-09-01 00:00:00
abstract：:We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization d...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0396-303
更新日期：1996-03-01 00:00:00
abstract：:We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, acti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/13732
更新日期：1999-10-01 00:00:00
abstract：:Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3440
更新日期：2016-02-01 00:00:00
abstract：:New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis of heterogeneous tissues. However, because these technologies can measure dozens of parameters simultaneously in individual cells, data interpretation can be challenging. Here we present viSNE, a tool that allows one to map h...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2594
更新日期：2013-06-01 00:00:00
abstract：:Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/82414
更新日期：2000-12-01 00:00:00
abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1393
更新日期：2008-04-01 00:00:00
abstract：:Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1170
更新日期：2006-01-01 00:00:00
abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0508-519
更新日期：2008-05-01 00:00:00
abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1211
更新日期：2006-06-01 00:00:00
abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0465-8
更新日期：2020-06-01 00:00:00
abstract：:Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4042
更新日期：2018-01-01 00:00:00
abstract：:N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0572-6
更新日期：2020-12-01 00:00:00
abstract：:The site-specific recombinases FLP and Cre are useful for genomic engineering in many living systems. Manipulation of their enzymatic properties offers a means to improve their applicability in different host organisms. We chose to manipulate the thermolability of FLP recombinase. A lacZ-based recombination assay in E...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0798-657
更新日期：1998-07-01 00:00:00
abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1611
更新日期：2010-03-01 00:00:00
abstract：:We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3886
更新日期：2017-07-01 00:00:00
abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1599
更新日期：2010-01-01 00:00:00
abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/76469
更新日期：2000-06-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0253-5
更新日期：2019-09-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt792
更新日期：2003-03-01 00:00:00
abstract：:MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1618
更新日期：2010-04-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41587-020-0457-8
更新日期：2020-03-01 00:00:00
abstract：:Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/89305
更新日期：2001-06-01 00:00:00
abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0696-731
更新日期：1996-06-01 00:00:00
abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/72671
更新日期：2000-02-01 00:00:00
abstract：:Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1511
更新日期：2008-12-01 00:00:00
abstract：:Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2689
更新日期：2013-11-01 00:00:00
abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0897-759
更新日期：1997-08-01 00:00:00
abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6146
更新日期：1999-02-01 00:00:00
