Presymptomatic visualization of plant-virus interactions by thermography.

Abstract:

:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be detectable when SA is synthesized locally in plant leaves. Here, resistance to tobacco mosaic virus (TMV) was monitored thermographically before any disease symptoms became visible on tobacco leaves. Spots of elevated temperature that were confined to the place of infection increased in intensity from 8 h before the onset of visible cell death, and remained detectable as a halo around the ongoing necrosis. Salicylic acid accumulates during the prenecrotic phase in TMV-infected tobacco and is known to induce stomatal closure in certain species. We show that the time course of SA accumulation correlates with the evolution of both localized thermal effect and stomatal closure. Since the contribution of leaf respiration is marginal, we concluded that the thermal effect results predominantly from localized, SA-induced stomatal closure. The presymptomatic temperature increase could be of general significance in incompatible plant-pathogen interactions.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Chaerle L,Van Caeneghem W,Messens E,Lambers H,Van Montagu M,Van Der Straeten D

doi

10.1038/11765

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

813-6

issue

8

eissn

1087-0156

issn

1546-1696

journal_volume

17

pub_type

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