Purification of recombinant proteins by fusion with thermally-responsive polypeptides.

abstract：:Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strai...

journal_title：Nature biotechnology

authors： Rongvaux A,Willinger T,Martinek J,Strowig T,Gearty SV,Teichmann LL,Saito Y,Marches F,Halene S,Palucka AK,Manz MG,Flavell RA

更新日期：2014-04-01 00:00:00

abstract：:Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...

journal_title：Nature biotechnology

authors： Khetani SR,Bhatia SN

更新日期：2008-01-01 00:00:00

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE site...

journal_title：Nature biotechnology

authors： Li X,Eastman EM,Schwartz RJ,Draghia-Akli R

更新日期：1999-03-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Thuronyi BW,Koblan LW,Levy JM,Yeh WH,Zheng C,Newby GA,Wilson C,Bhaumik M,Shubina-Oleinik O,Holt JR,Liu DR

更新日期：2019-09-01 00:00:00

abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...

journal_title：Nature biotechnology

authors： Bothmann H,Plückthun A

更新日期：1998-04-01 00:00:00

abstract：:Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available. Here we report the generation of induced ...

journal_title：Nature biotechnology

authors： Yang N,Zuchero JB,Ahlenius H,Marro S,Ng YH,Vierbuchen T,Hawkins JS,Geissler R,Barres BA,Wernig M

更新日期：2013-05-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumo...

journal_title：Nature biotechnology

authors： Pulido J,Kottke T,Thompson J,Galivo F,Wongthida P,Diaz RM,Rommelfanger D,Ilett E,Pease L,Pandha H,Harrington K,Selby P,Melcher A,Vile R

更新日期：2012-03-18 00:00:00

abstract：:Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...

journal_title：Nature biotechnology

authors： Kyung T,Lee S,Kim JE,Cho T,Park H,Jeong YM,Kim D,Shin A,Kim S,Baek J,Kim J,Kim NY,Woo D,Chae S,Kim CH,Shin HS,Han YM,Kim D,Heo WD

更新日期：2015-10-01 00:00:00

abstract：:Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout t...

journal_title：Nature biotechnology

authors： Rinner O,Mueller LN,Hubálek M,Müller M,Gstaiger M,Aebersold R

更新日期：2007-03-01 00:00:00

abstract：:Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...

journal_title：Nature biotechnology

authors： Krusche P,Trigg L,Boutros PC,Mason CE,De La Vega FM,Moore BL,Gonzalez-Porta M,Eberle MA,Tezak Z,Lababidi S,Truty R,Asimenos G,Funke B,Fleharty M,Chapman BA,Salit M,Zook JM,Global Alliance for Genomics and Health Benchma

更新日期：2019-05-01 00:00:00

abstract：:Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...

journal_title：Nature biotechnology

authors： Jhaveri S,Rajendran M,Ellington AD

更新日期：2000-12-01 00:00:00

abstract：:The basics of determining valuation will help you to understand what criteria matter most for investors. ...

journal_title：Nature biotechnology

authors： Frei P,Leleux B

更新日期：2004-08-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...

journal_title：Nature biotechnology

authors： Xu RH,Chen X,Li DS,Li R,Addicks GC,Glennon C,Zwaka TP,Thomson JA

更新日期：2002-12-01 00:00:00

abstract：:The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...

journal_title：Nature biotechnology

authors： Rissin DM,Kan CW,Campbell TG,Howes SC,Fournier DR,Song L,Piech T,Patel PP,Chang L,Rivnak AJ,Ferrell EP,Randall JD,Provuncher GK,Walt DR,Duffy DC

更新日期：2010-06-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...

journal_title：Nature biotechnology

authors： Shojaei F,Wu X,Malik AK,Zhong C,Baldwin ME,Schanz S,Fuh G,Gerber HP,Ferrara N

更新日期：2007-08-01 00:00:00

abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...

journal_title：Nature biotechnology

authors： Hoebe RA,Van Oven CH,Gadella TW Jr,Dhonukshe PB,Van Noorden CJ,Manders EM

更新日期：2007-02-01 00:00:00

abstract：:Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crys...

journal_title：Nature biotechnology

authors： Moellenbeck DJ,Peters ML,Bing JW,Rouse JR,Higgins LS,Sims L,Nevshemal T,Marshall L,Ellis RT,Bystrak PG,Lang BA,Stewart JL,Kouba K,Sondag V,Gustafson V,Nour K,Xu D,Swenson J,Zhang J,Czapla T,Schwab G,Jayne S,St

更新日期：2001-07-01 00:00:00

abstract：:Ensuring adequate manufacturing capacity for recombinant and monoclonal antibodies is one of the greatest challenges facing the biotech industry. The following factors should be taken into account when designing product development and production strategies. ...

journal_title：Nature biotechnology

authors： Carson KL

更新日期：2005-09-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) that have the potential to elicit new bone in vivo have been used in a tissue-engineering approach for the repair of bone injuries and bone defects. Although it is now possible to generate large amounts of recombinant human (rh) BMPs for medical use, the major challenge remains in th...

journal_title：Nature biotechnology

authors： Saito N,Okada T,Horiuchi H,Murakami N,Takahashi J,Nawata M,Ota H,Nozaki K,Takaoka K

更新日期：2001-04-01 00:00:00

abstract：:A variety of strategies to incorporate unnatural amino acids into proteins have been pursued, but all have limitations with respect to technical accessibility, scalability, applicability to in vivo studies, or site specificity of amino acid incorporation. The ability to selectively introduce unnatural functional group...

journal_title：Nature biotechnology

authors： Santoro SW,Wang L,Herberich B,King DS,Schultz PG

更新日期：2002-10-01 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...

journal_title：Nature biotechnology

authors： O'Rourke KP,Loizou E,Livshits G,Schatoff EM,Baslan T,Manchado E,Simon J,Romesser PB,Leach B,Han T,Pauli C,Beltran H,Rubin MA,Dow LE,Lowe SW

更新日期：2017-06-01 00:00:00

abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...

journal_title：Nature biotechnology

authors： Oberpenning F,Meng J,Yoo JJ,Atala A

更新日期：1999-02-01 00:00:00

abstract：:Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...

journal_title：Nature biotechnology

authors： Gat-Viks I,Chevrier N,Wilentzik R,Eisenhaure T,Raychowdhury R,Steuerman Y,Shalek AK,Hacohen N,Amit I,Regev A

更新日期：2013-04-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Röst HL,Rosenberger G,Navarro P,Gillet L,Miladinović SM,Schubert OT,Wolski W,Collins BC,Malmström J,Malmström L,Aebersold R

更新日期：2020-03-01 00:00:00

abstract：:DNA microarrays have the ability to analyze the expression of thousands of the same set of genes under at least two different experimental conditions. However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial tota...

journal_title：Nature biotechnology

authors： Talaat AM,Hunter P,Johnston SA

更新日期：2000-06-01 00:00:00

abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

journal_title：Nature biotechnology

authors： Bzikadze AV,Pevzner PA

更新日期：2020-11-01 00:00:00