Abstract：
:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a known limit and can form advanced derivatives of all three embryonic germ layers. What is less widely appreciated is that human ES cells can also form the extra-embryonic tissues that differentiate from the embryo before gastrulation. The use of human ES cells to derive early human trophoblast is particularly valuable, because it is difficult to obtain from other sources and is significantly different from mouse trophoblast. Here we show that bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor-beta (TGF-beta) superfamily, induces the differentiation of human ES cells to trophoblast. DNA microarray, RT-PCR, and immunoassay analyses demonstrate that the differentiated cells express a range of trophoblast markers and secrete placental hormones. When plated at low density, the BMP4-treated cells form syncytia that express chorionic gonadotrophin (CG). These results underscore fundamental differences between human and mouse ES cells, which differentiate poorly, if at all, to trophoblast. Human ES cells thus provide a tool for studying the differentiation and function of early human trophoblast and could provide a new understanding of some of the earliest differentiation events of human postimplantation development.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Xu RH,Chen X,Li DS,Li R,Addicks GC,Glennon C,Zwaka TP,Thomson JAdoi
10.1038/nbt761subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1261-4issue
12eissn
1087-0156issn
1546-1696pii
nbt761journal_volume
20pub_type
abstract：:Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...
journal_title：Nature biotechnology
pub_type： 杂志文章
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abstract：:As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...
journal_title：Nature biotechnology
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abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
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abstract：:Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism is widely used in the manufacture of fermented meats and has biotechnological potential in biopreservation and food safety. We have explored the 1,884,661-base-pair (bp) circular chromosome of strain...
journal_title：Nature biotechnology
pub_type： 杂志文章
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journal_title：Nature biotechnology
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doi：10.1038/76469
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journal_title：Nature biotechnology
pub_type： 杂志文章
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journal_title：Nature biotechnology
pub_type： 杂志文章,评审
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更新日期：1998-11-01 00:00:00
abstract：:Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2002-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/nbt1355
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journal_title：Nature biotechnology
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更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/nbt836
更新日期：2003-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
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pub_type： 新闻
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更新日期：2010-10-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/81192
更新日期：2000-11-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt1369
更新日期：2007-12-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0598-431
更新日期：1998-05-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1009
更新日期：2004-09-01 00:00:00
abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1611
更新日期：2010-03-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0292-y
更新日期：2019-11-01 00:00:00
abstract：:For the past five decades, the need for new antibiotics has been met largely by semisynthetic tailoring of natural product scaffolds discovered in the middle of the 20(th) century. More recently, however, advances in technology have sparked a resurgence in the discovery of natural product antibiotics from bacterial so...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt1266
更新日期：2006-12-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1206-1504
更新日期：2006-12-01 00:00:00
abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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更新日期：2004-01-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/8666
更新日期：1999-05-01 00:00:00
abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1211
更新日期：2006-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3412
更新日期：2015-12-01 00:00:00
abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3902
更新日期：2017-09-01 00:00:00
abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6146
更新日期：1999-02-01 00:00:00
