Abstract:
:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have received much attention and several compounds are currently being tested in clinical trials. Although induced RNAi is able to trigger profound and specific inhibition of virus replication, it is becoming clear that RNAi therapeutics are not as straightforward as we had initially hoped. Difficulties concerning toxicity and delivery to the right cells that earlier hampered the development of antisense-based therapeutics may also apply to RNAi. In addition, there are indications that viruses have evolved ways to escape from RNAi. Proper consideration of all of these issues will be necessary in the design of RNAi-based therapeutics for successful clinical intervention of human pathogenic viruses.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Haasnoot J,Westerhout EM,Berkhout Bdoi
10.1038/nbt1369subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1435-43issue
12eissn
1087-0156issn
1546-1696pii
nbt1369journal_volume
25pub_type
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journal_title:Nature biotechnology
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abstract::Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...
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更新日期:2008-05-01 00:00:00
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pub_type: 已发布勘误
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更新日期:2019-12-01 00:00:00
abstract::The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...
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pub_type: 杂志文章
doi:10.1038/nbt1355
更新日期:2007-12-01 00:00:00
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journal_title:Nature biotechnology
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
abstract::Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...
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更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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journal_title:Nature biotechnology
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更新日期:1999-06-01 00:00:00
abstract::The treatment of asthma and allergic rhinitis using unique, humanized anti-IgE monoclonal antibodies with very particular binding specificities is now supported by the results of multiple phase II and III human clinical studies. The therapeutic efficacy of this approach is attributable to several pharmacological mecha...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
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abstract::Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...
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更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1996-09-01 00:00:00
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更新日期:2013-11-01 00:00:00
abstract::Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...
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更新日期:2018-10-01 00:00:00