Abstract：
:The treatment of asthma and allergic rhinitis using unique, humanized anti-IgE monoclonal antibodies with very particular binding specificities is now supported by the results of multiple phase II and III human clinical studies. The therapeutic efficacy of this approach is attributable to several pharmacological mechanisms. In addition to the expected effects of these monoclonal antibodies in neutralizing free IgE and inhibiting IgE production by B cells, several indirect biochemical and cellular effects have been uncovered during the course of the clinical trials. These include the accumulation of potentially beneficial IgE-anti-IgE immune complexes and the downregulation of the high-affinity IgE Fc receptors (FcvarepsilonRI) on basophils and mast cells. This article analyzes the structural basis of the specificity of the anti-IgE antibodies and pertinent results from in vitro experiments, animal model studies, and human clinical trials in an attempt to provide a cogent pharmacological interpretation of the therapeutic effects of anti-IgE therapy in both the near- and long term. The development of anti-IgE therapy over the past 10 years provides an interesting example of the emergence of a conceptually new, biotechnology-produced pharmaceutical.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Chang TWdoi
10.1038/72601subject
Has Abstractpub_date
2000-02-01 00:00:00pages
157-62issue
2eissn
1087-0156issn
1546-1696journal_volume
18pub_type
杂志文章,评审abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0067-5
更新日期：2019-04-01 00:00:00
abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3199
更新日期：2015-05-01 00:00:00
abstract：:Academic researchers have regularly ignored patents on key technologies as a strategy to maneuver around patent thickets and freedom-to-operate issues, but they may be more at risk than they realize. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1107-1225
更新日期：2007-11-01 00:00:00
abstract：:Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4201
更新日期：2018-10-01 00:00:00
abstract：:Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/82414
更新日期：2000-12-01 00:00:00
abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1206-1504
更新日期：2006-12-01 00:00:00
abstract：:Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0293-x
更新日期：2019-12-01 00:00:00
abstract：:The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnif...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3641
更新日期：2016-09-01 00:00:00
abstract：:Structural genomics has the ambitious goal of delivering three-dimensional structural information on a genome-wide scale. Yet only a small fraction of natural proteins are suitable for structure determination because of bottlenecks such as poor expression, aggregation, and misfolding of proteins, and difficulties in s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt732
更新日期：2002-09-01 00:00:00
abstract：:The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2620
更新日期：2013-07-01 00:00:00
abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0198-33
更新日期：1998-01-01 00:00:00
abstract：:Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0592-2
更新日期：2021-01-01 00:00:00
abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0208-183
更新日期：2008-02-01 00:00:00
abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/84422
更新日期：2001-02-01 00:00:00
abstract：:Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE site...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6981
更新日期：1999-03-01 00:00:00
abstract：:As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0506-517
更新日期：2006-05-01 00:00:00
abstract：:Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3350
更新日期：2015-10-01 00:00:00
abstract：:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is a powerful method to quickly and accurately determine the masses of peptides. Most genetic analyses, however, begin with PCR amplification of a test sequence to generate DNA, which is more difficult than peptides to analyze by ...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/72013
更新日期：2000-01-01 00:00:00
abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0322-9
更新日期：2020-02-01 00:00:00
abstract：:Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2195
更新日期：2012-05-13 00:00:00
abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...
journal_title：Nature biotechnology
pub_type： 新闻
doi：10.1038/nbt.1768
更新日期：2011-02-01 00:00:00
abstract：:The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein inter...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt816
更新日期：2003-05-01 00:00:00
abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0656-3
更新日期：2020-09-07 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0167-2
更新日期：2019-07-01 00:00:00
abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1306
更新日期：2007-06-01 00:00:00
abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2862
更新日期：2014-05-01 00:00:00
abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1298
更新日期：2007-04-01 00:00:00
abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
abstract：:Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4042
更新日期：2018-01-01 00:00:00
abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0465-8
更新日期：2020-06-01 00:00:00
