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Bacterial glycosidases for the production of universal red blood cells.

Abstract：

:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD+. The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

doi

10.1038/nbt1298

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

454-64

issue

4

eissn

1087-0156

issn

1546-1696

pii

nbt1298

journal_volume

25

pub_type

杂志文章

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