Abstract：
:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Hosaka T,Ohnishi-Kameyama M,Muramatsu H,Murakami K,Tsurumi Y,Kodani S,Yoshida M,Fujie A,Ochi Kdoi
10.1038/nbt.1538subject
Has Abstractpub_date
2009-05-01 00:00:00pages
462-4issue
5eissn
1087-0156issn
1546-1696pii
nbt.1538journal_volume
27pub_type
杂志文章abstract：:Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient exp...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2024
更新日期：2011-11-13 00:00:00
abstract：:The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt872
更新日期：2003-10-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, rob...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1629
更新日期：2010-06-01 00:00:00
abstract：:We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/85712
更新日期：2001-03-01 00:00:00
abstract：:A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0628-7
更新日期：2021-01-01 00:00:00
abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4052
更新日期：2018-02-01 00:00:00
abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0322-9
更新日期：2020-02-01 00:00:00
abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.4022
更新日期：2017-12-01 00:00:00
abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/70761
更新日期：1999-12-01 00:00:00
abstract：:Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism is widely used in the manufacture of fermented meats and has biotechnological potential in biopreservation and food safety. We have explored the 1,884,661-base-pair (bp) circular chromosome of strain...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1160
更新日期：2005-12-01 00:00:00
abstract：:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0898-733
更新日期：1998-08-01 00:00:00
abstract：:Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1216
更新日期：2006-06-01 00:00:00
abstract：:Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3440
更新日期：2016-02-01 00:00:00
abstract：:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1520
更新日期：2009-02-01 00:00:00
abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt761
更新日期：2002-12-01 00:00:00
abstract：:Ensuring adequate manufacturing capacity for recombinant and monoclonal antibodies is one of the greatest challenges facing the biotech industry. The following factors should be taken into account when designing product development and production strategies. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0905-1054
更新日期：2005-09-01 00:00:00
abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/88084
更新日期：2001-05-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-020-0658-1
更新日期：2020-09-01 00:00:00
abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0797-663
更新日期：1997-07-01 00:00:00
abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2798
更新日期：2014-02-01 00:00:00
abstract：:The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/74455
更新日期：2000-04-01 00:00:00
abstract：:Targeting of secreted and cell-surface proteins to the cell membrane is mediated by a short hydrophobic stretch of amino acids, termed the signal sequence. We have developed a method that detects signal sequences in cDNA fragments based on their ability to redirect a constitutively active mutant of a cytokine receptor...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/8666
更新日期：1999-05-01 00:00:00
abstract：:We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1007
更新日期：2004-09-01 00:00:00
abstract：:A Pennsylvania company hopes to turn synthetic DNA vaccines into rapid response agents against flu epidemics and cancer. ...
journal_title：Nature biotechnology
pub_type： 新闻
doi：10.1038/nbt0213-98
更新日期：2013-02-01 00:00:00
abstract：:The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1632
更新日期：2010-05-01 00:00:00
abstract：:Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million to...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/74531
更新日期：2000-04-01 00:00:00
abstract：:Replicate mass spectrometry (MS) measurements and the use of multiple analytical methods can greatly expand the comprehensiveness of shotgun proteomic profiling of biological samples. However, the inherent biases and variations in such data create computational and statistical challenges for quantitative comparative a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1592
更新日期：2010-01-01 00:00:00
abstract：:Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-termini of their protease cleavage products would accelerate identification of the substrates of proteases with broad or unknown specificity. Our approach, named terminal amine isotopic labeling of substrates (TAILS), addresses th...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1611
更新日期：2010-03-01 00:00:00
abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0067-5
更新日期：2019-04-01 00:00:00
