Abstract:
:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the novel ligand structure of the metal-binding sites, the unique mechanism of the enzyme hyper-induction, and the occurrence of an accessory gene involved in cobalt-containing NHase formation. These detailed analyses have led to the development of biotechnological applications of NHase, including biotransformation and bioremediation.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Kobayashi M,Shimizu Sdoi
10.1038/nbt0898-733subject
Has Abstractpub_date
1998-08-01 00:00:00pages
733-6issue
8eissn
1087-0156issn
1546-1696journal_volume
16pub_type
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