Abstract:
:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 times faster) without loss of accuracy. By facilitating frequent reanalysis of data and reducing the need to optimize parameters, Sailfish exemplifies the potential of lightweight algorithms for efficiently processing sequencing reads.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Patro R,Mount SM,Kingsford Cdoi
10.1038/nbt.2862subject
Has Abstractpub_date
2014-05-01 00:00:00pages
462-4issue
5eissn
1087-0156issn
1546-1696pii
nbt.2862journal_volume
32pub_type
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