Alteration of Cre recombinase site specificity by substrate-linked protein evolution.

abstract：:Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...

journal_title：Nature biotechnology

authors： Branon TC,Bosch JA,Sanchez AD,Udeshi ND,Svinkina T,Carr SA,Feldman JL,Perrimon N,Ting AY

更新日期：2018-10-01 00:00:00

abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...

journal_title：Nature biotechnology

authors：

更新日期：2009-09-01 00:00:00

abstract：:The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...

journal_title：Nature biotechnology

authors： Zhu L,van de Lavoir MC,Albanese J,Beenhouwer DO,Cardarelli PM,Cuison S,Deng DF,Deshpande S,Diamond JH,Green L,Halk EL,Heyer BS,Kay RM,Kerchner A,Leighton PA,Mather CM,Morrison SL,Nikolov ZL,Passmore DB,Pradas-Monne

更新日期：2005-09-01 00:00:00

abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...

journal_title：Nature biotechnology

authors： Lewis K,Ausubel FM

更新日期：2006-12-01 00:00:00

abstract：:A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essentia...

journal_title：Nature biotechnology

authors： Orchard S,Salwinski L,Kerrien S,Montecchi-Palazzi L,Oesterheld M,Stümpflen V,Ceol A,Chatr-aryamontri A,Armstrong J,Woollard P,Salama JJ,Moore S,Wojcik J,Bader GD,Vidal M,Cusick ME,Gerstein M,Gavin AC,Superti-Furga G,

更新日期：2007-08-01 00:00:00

abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...

journal_title：Nature biotechnology

authors： Oberpenning F,Meng J,Yoo JJ,Atala A

更新日期：1999-02-01 00:00:00

abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...

journal_title：Nature biotechnology

authors： Zhang L,Miles MF,Aldape KD

更新日期：2003-07-01 00:00:00

abstract：:Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...

journal_title：Nature biotechnology

authors： Montini E,Cesana D,Schmidt M,Sanvito F,Ponzoni M,Bartholomae C,Sergi Sergi L,Benedicenti F,Ambrosi A,Di Serio C,Doglioni C,von Kalle C,Naldini L

更新日期：2006-06-01 00:00:00

abstract：:Pluripotent stem cell-derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal m...

journal_title：Nature biotechnology

authors： Liu YW,Chen B,Yang X,Fugate JA,Kalucki FA,Futakuchi-Tsuchida A,Couture L,Vogel KW,Astley CA,Baldessari A,Ogle J,Don CW,Steinberg ZL,Seslar SP,Tuck SA,Tsuchida H,Naumova AV,Dupras SK,Lyu MS,Lee J,Hailey DW,Reinec

更新日期：2018-08-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...

journal_title：Nature biotechnology

authors： Sultan P,Schechner JS,McNiff JM,Hochman PS,Hughes CC,Lorber MI,Askenase PW,Pober JS

更新日期：1997-08-01 00:00:00

abstract：:Structural genomics has the ambitious goal of delivering three-dimensional structural information on a genome-wide scale. Yet only a small fraction of natural proteins are suitable for structure determination because of bottlenecks such as poor expression, aggregation, and misfolding of proteins, and difficulties in s...

journal_title：Nature biotechnology

authors： Pédelacq JD,Piltch E,Liong EC,Berendzen J,Kim CY,Rho BS,Park MS,Terwilliger TC,Waldo GS

更新日期：2002-09-01 00:00:00

abstract：:The ability to produce monoclonal antibodies (Mabs) in plants offers the opportunity for the development of an inexpensive method of mucosal immunoprotection against sexually transmitted diseases. To investigate the suitability of plant-expressed Mabs for vaginal preventive applications, we compared a humanized anti-h...

journal_title：Nature biotechnology

authors： Zeitlin L,Olmsted SS,Moench TR,Co MS,Martinell BJ,Paradkar VM,Russell DR,Queen C,Cone RA,Whaley KJ

更新日期：1998-12-01 00:00:00

abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...

journal_title：Nature biotechnology

authors： Deng J,Shoemaker R,Xie B,Gore A,LeProust EM,Antosiewicz-Bourget J,Egli D,Maherali N,Park IH,Yu J,Daley GQ,Eggan K,Hochedlinger K,Thomson J,Wang W,Gao Y,Zhang K

更新日期：2009-04-01 00:00:00

abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...

journal_title：Nature biotechnology

authors： Bothmann H,Plückthun A

更新日期：1998-04-01 00:00:00

abstract：:Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...

journal_title：Nature biotechnology

authors： Chick JM,Kolippakkam D,Nusinow DP,Zhai B,Rad R,Huttlin EL,Gygi SP

更新日期：2015-07-01 00:00:00

abstract：:Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...

journal_title：Nature biotechnology

authors： Sönnichsen C,Reinhard BM,Liphardt J,Alivisatos AP

更新日期：2005-06-01 00:00:00

abstract：:The functional state of a cell is largely determined by the spatiotemporal organization of its proteome. Technologies exist for measuring particular aspects of protein turnover and localization, but comprehensive analysis of protein dynamics across different scales is possible only by combining several methods. Here w...

journal_title：Nature biotechnology

authors： Khmelinskii A,Keller PJ,Bartosik A,Meurer M,Barry JD,Mardin BR,Kaufmann A,Trautmann S,Wachsmuth M,Pereira G,Huber W,Schiebel E,Knop M

更新日期：2012-06-24 00:00:00

abstract：:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...

journal_title：Nature biotechnology

authors： Thepen T,van Vuuren AJ,Kiekens RC,Damen CA,Vooijs WC,van De Winkel JG

更新日期：2000-01-01 00:00:00

abstract：:CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...

journal_title：Nature biotechnology

authors： Doench JG,Fusi N,Sullender M,Hegde M,Vaimberg EW,Donovan KF,Smith I,Tothova Z,Wilen C,Orchard R,Virgin HW,Listgarten J,Root DE

更新日期：2016-02-01 00:00:00

abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...

journal_title：Nature biotechnology

authors： Schafer AJ,Hawkins JR

更新日期：1998-01-01 00:00:00

abstract：:In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. ...

journal_title：Nature biotechnology

authors： Dolgin E

更新日期：2019-02-01 00:00:00

abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

journal_title：Nature biotechnology

authors： Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

更新日期：2018-02-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Thuronyi BW,Koblan LW,Levy JM,Yeh WH,Zheng C,Newby GA,Wilson C,Bhaumik M,Shubina-Oleinik O,Holt JR,Liu DR

更新日期：2019-09-01 00:00:00

abstract：:Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of single-particle images. For small (<100 kDa) DNA-binding proteins, obtaining particle images with sufficiently asymmetric features to correctly guide alignment is challenging....

journal_title：Nature biotechnology

authors： Aksel T,Yu Z,Cheng Y,Douglas SM

更新日期：2020-10-19 00:00:00

abstract：:We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region...

journal_title：Nature biotechnology

authors： Lunde E,Munthe LA,Vabø A,Sandlie I,Bogen B

更新日期：1999-07-01 00:00:00

abstract：:We show that transplantation of adult bone marrow-derived cells expressing c-kit reduces hyperglycemia in mice with streptozotocin-induced pancreatic damage. Although quantitative analysis of the pancreas revealed a low frequency of donor insulin-positive cells, these cells were not present at the onset of blood gluco...

journal_title：Nature biotechnology

authors： Hess D,Li L,Martin M,Sakano S,Hill D,Strutt B,Thyssen S,Gray DA,Bhatia M

更新日期：2003-07-01 00:00:00

abstract：:Recent therapeutic successes have renewed interest in drug combinations, but experimental screening approaches are costly and often identify only small numbers of synergistic combinations. The DREAM consortium launched an open challenge to foster the development of in silico methods to computationally rank 91 compound...

journal_title：Nature biotechnology

authors： Bansal M,Yang J,Karan C,Menden MP,Costello JC,Tang H,Xiao G,Li Y,Allen J,Zhong R,Chen B,Kim M,Wang T,Heiser LM,Realubit R,Mattioli M,Alvarez MJ,Shen Y,NCI-DREAM Community.,Gallahan D,Singer D,Saez-Rodriguez J,

更新日期：2014-12-01 00:00:00

abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...

journal_title：Nature biotechnology

authors： Eisenstein M

更新日期：2011-02-01 00:00:00

abstract：:We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...

journal_title：Nature biotechnology

authors： Marszalek PE,Li H,Fernandez JM

更新日期：2001-03-01 00:00:00