Abstract：
:The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs. To accommodate these large regulatory regions, we established and transfected lines of chicken embryonic stem (cES) cells and formed chimeras that express mAb from cES cell-derived tubular gland cells. Eggs from high-grade chimeras contained up to 3 mg of mAb that possesses enhanced antibody-dependent cellular cytotoxicity (ADCC), nonantigenic glycosylation, acceptable half-life, excellent antigen recognition and good rates of internalization.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Zhu L,van de Lavoir MC,Albanese J,Beenhouwer DO,Cardarelli PM,Cuison S,Deng DF,Deshpande S,Diamond JH,Green L,Halk EL,Heyer BS,Kay RM,Kerchner A,Leighton PA,Mather CM,Morrison SL,Nikolov ZL,Passmore DB,Pradas-Monnedoi
10.1038/nbt1132subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1159-69issue
9eissn
1087-0156issn
1546-1696pii
nbt1132journal_volume
23pub_type
杂志文章abstract：:We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1077
更新日期：2005-04-01 00:00:00
abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/70761
更新日期：1999-12-01 00:00:00
abstract：:Replicate mass spectrometry (MS) measurements and the use of multiple analytical methods can greatly expand the comprehensiveness of shotgun proteomic profiling of biological samples. However, the inherent biases and variations in such data create computational and statistical challenges for quantitative comparative a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1592
更新日期：2010-01-01 00:00:00
abstract：:The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt802
更新日期：2003-04-01 00:00:00
abstract：:Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0392-8
更新日期：2020-03-01 00:00:00
abstract：:Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a ce...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0202-149
更新日期：2002-02-01 00:00:00
abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0498-376
更新日期：1998-04-01 00:00:00
abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt943
更新日期：2004-03-01 00:00:00
abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.4022
更新日期：2017-12-01 00:00:00
abstract：:Epigenetic mechanisms integrate genetic and environmental causes of disease, but comprehensive genome-wide analyses of epigenetic modifications have not yet demonstrated robust association with common diseases. Using Illumina HumanMethylation450 arrays on 354 anti-citrullinated protein antibody-associated rheumatoid a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2487
更新日期：2013-02-01 00:00:00
abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt836
更新日期：2003-07-01 00:00:00
abstract：:The potential therapeutic applications of encapsulated cells are enormous. In the US alone, it has been estimated that nearly half-a-trillion dollars are spent each year to care for patients who suffer tissue loss or dysfunction. Over 6 million patients suffer from neurodegenerative disorders such as Alzheimer's disea...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0996-1107
更新日期：1996-09-01 00:00:00
abstract：:Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0037-y
更新日期：2019-03-01 00:00:00
abstract：:RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2916
更新日期：2014-07-01 00:00:00
abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0897-759
更新日期：1997-08-01 00:00:00
abstract：:Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE site...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6981
更新日期：1999-03-01 00:00:00
abstract：:The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes compr...
journal_title：Nature biotechnology
pub_type： 信件
doi：10.1038/nbt1036
更新日期：2004-12-01 00:00:00
abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2768
更新日期：2013-12-01 00:00:00
abstract：:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/71908
更新日期：2000-01-01 00:00:00
abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/76469
更新日期：2000-06-01 00:00:00
abstract：:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1520
更新日期：2009-02-01 00:00:00
abstract：:Structural genomics has the ambitious goal of delivering three-dimensional structural information on a genome-wide scale. Yet only a small fraction of natural proteins are suitable for structure determination because of bottlenecks such as poor expression, aggregation, and misfolding of proteins, and difficulties in s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt732
更新日期：2002-09-01 00:00:00
abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1259
更新日期：2006-11-01 00:00:00
abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0197-74
更新日期：1997-01-01 00:00:00
abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4052
更新日期：2018-02-01 00:00:00
abstract：:We report a protocol for the rapid identification of DNA-binding proteins. Immobilized DNA probes harboring a specific sequence motif are incubated with cell or nuclear extract. Proteins are analyzed directly off the solid support by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The det...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/12873
更新日期：1999-09-01 00:00:00
abstract：:The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single ce...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3880
更新日期：2017-07-01 00:00:00
abstract：:Mycobacterium tuberculosis is a natural mutant in oxyR, a close homolog of the central regulator of peroxide stress response in enteric bacteria. Inactivation of oxyR is specific for M. tuberculosis and other members of the M. tuberculosis complex. This phenomenon appears as a paradox due to the ability of this organi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1196-1557
更新日期：1996-11-01 00:00:00
abstract：:MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1618
更新日期：2010-04-01 00:00:00
abstract：:The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1162
更新日期：2005-12-01 00:00:00
