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A model of molecular interactions on short oligonucleotide microarrays.

Abstract：

:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequence specificity or low sensitivity. Here, we describe a model of binding interactions on microarrays that reveals how probe signals depend on probe sequences and why certain probes are ineffective. The model indicates that the amount of nonspecific binding can be estimated from a simple rule. Using this model, we have developed an improved measure of gene expression for use in data analysis.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Zhang L,Miles MF,Aldape KD

doi

10.1038/nbt836

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

818-21

issue

7

eissn

1087-0156

issn

1546-1696

pii

nbt836

journal_volume

21

pub_type

杂志文章

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