Abstract：
:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conformation capture-based experiments, can be used as genomic distance proxies to accurately position individual contigs without requiring any sequence overlap. We also use these data to construct approximate genome scaffolds de novo. Applying our approach to incomplete regions of the human genome, we predict the positions of 65 previously unplaced contigs, in agreement with alternative methods in 26/31 cases attempted in common. Our approach can theoretically bridge any gap size and should be applicable to any species for which global chromatin interaction data can be generated.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Kaplan N,Dekker Jdoi
10.1038/nbt.2768subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1143-7issue
12eissn
1087-0156issn
1546-1696pii
nbt.2768journal_volume
31pub_type
杂志文章abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...
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pub_type： 杂志文章
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journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2052
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abstract：:The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...
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pub_type： 新闻
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更新日期：1997-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/13732
更新日期：1999-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/83489
更新日期：2001-01-01 00:00:00
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