Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model.

abstract：:The ability to produce monoclonal antibodies (Mabs) in plants offers the opportunity for the development of an inexpensive method of mucosal immunoprotection against sexually transmitted diseases. To investigate the suitability of plant-expressed Mabs for vaginal preventive applications, we compared a humanized anti-h...

journal_title：Nature biotechnology

authors： Zeitlin L,Olmsted SS,Moench TR,Co MS,Martinell BJ,Paradkar VM,Russell DR,Queen C,Cone RA,Whaley KJ

更新日期：1998-12-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...

journal_title：Nature biotechnology

authors： Daniels GA,Sanchez-Perez L,Diaz RM,Kottke T,Thompson J,Lai M,Gough M,Karim M,Bushell A,Chong H,Melcher A,Harrington K,Vile RG

更新日期：2004-09-01 00:00:00

abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...

journal_title：Nature biotechnology

authors： Cheung AS,Zhang DKY,Koshy ST,Mooney DJ

更新日期：2018-02-01 00:00:00

abstract：:Genetic testing for rare heart conditions might someday expand to more common cardiac ailments. Already there are signs testing is dramatically changing how some conditions are treated and doctors' definition of who a patient is. Stephen Strauss reports. ...

journal_title：Nature biotechnology

authors： Strauss S

更新日期：2010-10-01 00:00:00

abstract：:Epigenetic mechanisms integrate genetic and environmental causes of disease, but comprehensive genome-wide analyses of epigenetic modifications have not yet demonstrated robust association with common diseases. Using Illumina HumanMethylation450 arrays on 354 anti-citrullinated protein antibody-associated rheumatoid a...

journal_title：Nature biotechnology

authors： Liu Y,Aryee MJ,Padyukov L,Fallin MD,Hesselberg E,Runarsson A,Reinius L,Acevedo N,Taub M,Ronninger M,Shchetynsky K,Scheynius A,Kere J,Alfredsson L,Klareskog L,Ekström TJ,Feinberg AP

更新日期：2013-02-01 00:00:00

abstract：:Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...

journal_title：Nature biotechnology

authors： Shimbo I,Nakajima R,Yokoyama S,Sumikura K

更新日期：2004-01-01 00:00:00

abstract：:Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanog...

journal_title：Nature biotechnology

authors： Brunner E,Ahrens CH,Mohanty S,Baetschmann H,Loevenich S,Potthast F,Deutsch EW,Panse C,de Lichtenberg U,Rinner O,Lee H,Pedrioli PG,Malmstrom J,Koehler K,Schrimpf S,Krijgsveld J,Kregenow F,Heck AJ,Hafen E,Schlapbach R

更新日期：2007-05-01 00:00:00

abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

journal_title：Nature biotechnology

authors： Kolkman JA,Stemmer WP

更新日期：2001-05-01 00:00:00

abstract：:A variety of strategies to incorporate unnatural amino acids into proteins have been pursued, but all have limitations with respect to technical accessibility, scalability, applicability to in vivo studies, or site specificity of amino acid incorporation. The ability to selectively introduce unnatural functional group...

journal_title：Nature biotechnology

authors： Santoro SW,Wang L,Herberich B,King DS,Schultz PG

更新日期：2002-10-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...

journal_title：Nature biotechnology

authors： Schafer AJ,Hawkins JR

更新日期：1998-01-01 00:00:00

abstract：:High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-inter...

journal_title：Nature biotechnology

authors： Evers B,Jastrzebski K,Heijmans JP,Grernrum W,Beijersbergen RL,Bernards R

更新日期：2016-06-01 00:00:00

abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...

journal_title：Nature biotechnology

authors： Barennes P,Quiniou V,Shugay M,Egorov ES,Davydov AN,Chudakov DM,Uddin I,Ismail M,Oakes T,Chain B,Eugster A,Kashofer K,Rainer PP,Darko S,Ransier A,Douek DC,Klatzmann D,Mariotti-Ferrandiz E

更新日期：2020-09-07 00:00:00

abstract：:Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...

journal_title：Nature biotechnology

authors： Yang XW,Model P,Heintz N

更新日期：1997-09-01 00:00:00

abstract：:Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crys...

journal_title：Nature biotechnology

authors： Moellenbeck DJ,Peters ML,Bing JW,Rouse JR,Higgins LS,Sims L,Nevshemal T,Marshall L,Ellis RT,Bystrak PG,Lang BA,Stewart JL,Kouba K,Sondag V,Gustafson V,Nour K,Xu D,Swenson J,Zhang J,Czapla T,Schwab G,Jayne S,St

更新日期：2001-07-01 00:00:00

abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...

journal_title：Nature biotechnology

authors： Wang Z,Zhu T,Qiao C,Zhou L,Wang B,Zhang J,Chen C,Li J,Xiao X

更新日期：2005-03-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:Recent therapeutic successes have renewed interest in drug combinations, but experimental screening approaches are costly and often identify only small numbers of synergistic combinations. The DREAM consortium launched an open challenge to foster the development of in silico methods to computationally rank 91 compound...

journal_title：Nature biotechnology

authors： Bansal M,Yang J,Karan C,Menden MP,Costello JC,Tang H,Xiao G,Li Y,Allen J,Zhong R,Chen B,Kim M,Wang T,Heiser LM,Realubit R,Mattioli M,Alvarez MJ,Shen Y,NCI-DREAM Community.,Gallahan D,Singer D,Saez-Rodriguez J,

更新日期：2014-12-01 00:00:00

abstract：:Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and ...

journal_title：Nature biotechnology

authors： Barrangou R,Doudna JA

更新日期：2016-01-01 00:00:00

abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...

journal_title：Nature biotechnology

authors： Ding J,Adiconis X,Simmons SK,Kowalczyk MS,Hession CC,Marjanovic ND,Hughes TK,Wadsworth MH,Burks T,Nguyen LT,Kwon JYH,Barak B,Ge W,Kedaigle AJ,Carroll S,Li S,Hacohen N,Rozenblatt-Rosen O,Shalek AK,Villani AC,Regev

更新日期：2020-06-01 00:00:00

abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...

journal_title：Nature biotechnology

authors： Giordano-Coltart J,Calkins CW

更新日期：2008-02-01 00:00:00

abstract：:A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient proces...

journal_title：Nature biotechnology

authors： Wittrup A,Ai A,Liu X,Hamar P,Trifonova R,Charisse K,Manoharan M,Kirchhausen T,Lieberman J

更新日期：2015-08-01 00:00:00

abstract：:To select for bacterial strains with enhanced phenotypes, random fragments of a whole genome, or a defined region of the genome, are cloned in a nonreplicating vector. The resulting plasmids are integrated by recombination into the homologous DNA region of the original strain. Integration gives rise to a nontandem dir...

journal_title：Nature biotechnology

authors： Mavingui P,Flores M,Romero D,Martínez-Romero E,Palacios R

更新日期：1997-06-01 00:00:00

abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...

journal_title：Nature biotechnology

authors： Lee D,Hyun JH,Jung K,Hannan P,Kwon HB

更新日期：2017-09-01 00:00:00

abstract：:Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...

journal_title：Nature biotechnology

authors： Richards M,Fong CY,Chan WK,Wong PC,Bongso A

更新日期：2002-09-01 00:00:00

abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

journal_title：Nature biotechnology

authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

更新日期：2012-12-01 00:00:00

abstract：:The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...

journal_title：Nature biotechnology

authors： Chen MT,Weiss R

更新日期：2005-12-01 00:00:00

abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...

journal_title：Nature biotechnology

authors： Sherwood RI,Hashimoto T,O'Donnell CW,Lewis S,Barkal AA,van Hoff JP,Karun V,Jaakkola T,Gifford DK

更新日期：2014-02-01 00:00:00

abstract：:DNA microarrays have the ability to analyze the expression of thousands of the same set of genes under at least two different experimental conditions. However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial tota...

journal_title：Nature biotechnology

authors： Talaat AM,Hunter P,Johnston SA

更新日期：2000-06-01 00:00:00