Abstract:
:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Giordano-Coltart J,Calkins CWdoi
10.1038/nbt0208-183subject
Has Abstractpub_date
2008-02-01 00:00:00pages
183-5issue
2eissn
1087-0156issn
1546-1696pii
nbt0208-183journal_volume
26pub_type
杂志文章abstract::Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1100
更新日期:2005-06-01 00:00:00
abstract::Epigenetic modifications constitute a complex regulatory layer on top of the genome sequence. Pluripotent and differentiated cells provide a powerful system for investigating how the epigenetic code influences cellular fate. High-throughput sequencing of these cell types has yielded DNA methylation maps at single-nucl...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.1684
更新日期:2010-10-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0292-y
更新日期:2019-11-01 00:00:00
abstract::Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/89305
更新日期:2001-06-01 00:00:00
abstract::Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3514
更新日期:2016-05-01 00:00:00
abstract::Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1278
更新日期:2007-02-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1323
更新日期:2007-08-01 00:00:00
abstract::A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0896-1003
更新日期:1996-08-01 00:00:00
abstract::RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...
journal_title:Nature biotechnology
pub_type:
doi:10.1038/nbt792
更新日期:2003-03-01 00:00:00
abstract::Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1298
更新日期:2007-04-01 00:00:00
abstract::Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0582-4
更新日期:2020-11-01 00:00:00
abstract::RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2916
更新日期:2014-07-01 00:00:00
abstract::The human transcriptome is marked by extensive alternative mRNA splicing and the expression of many closely related genes, which may be difficult to distinguish using standard microarray techniques. Here we describe a sensitive and specific assay for parallel analysis of mRNA isoforms on a fiber-optic microarray platf...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0402-353
更新日期:2002-04-01 00:00:00
abstract::Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0406-411
更新日期:2006-04-01 00:00:00
abstract::RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1541
更新日期:2009-06-01 00:00:00
abstract::High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt836
更新日期:2003-07-01 00:00:00
abstract::The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1197-1271
更新日期:1997-11-01 00:00:00
abstract::In most tree-breeding programs worldwide, increasing the trees' growth rates and stem volumes and shortening their rotation times are important aims. Such trees would yield more biomass per unit area. Here we show that overexpressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hy...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/77355
更新日期:2000-07-01 00:00:00
abstract::Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/78504
更新日期:2000-08-01 00:00:00
abstract::Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0298-196
更新日期:1998-02-01 00:00:00
abstract::Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4004
更新日期:2017-12-01 00:00:00
abstract::Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.4022
更新日期:2017-12-01 00:00:00
abstract::Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3440
更新日期:2016-02-01 00:00:00
abstract::We report a protocol for the rapid identification of DNA-binding proteins. Immobilized DNA probes harboring a specific sequence motif are incubated with cell or nuclear extract. Proteins are analyzed directly off the solid support by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The det...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/12873
更新日期:1999-09-01 00:00:00
abstract::Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. Stage (S) 7 cells expressed key markers of mature pancreatic...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3033
更新日期:2014-11-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/77296
更新日期:2000-07-01 00:00:00
abstract::The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single ce...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3880
更新日期:2017-07-01 00:00:00
abstract::The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/84422
更新日期:2001-02-01 00:00:00
abstract::Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0333-6
更新日期:2019-12-01 00:00:00
abstract::New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis of heterogeneous tissues. However, because these technologies can measure dozens of parameters simultaneously in individual cells, data interpretation can be challenging. Here we present viSNE, a tool that allows one to map h...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2594
更新日期:2013-06-01 00:00:00