Abstract：
:Epigenetic modifications constitute a complex regulatory layer on top of the genome sequence. Pluripotent and differentiated cells provide a powerful system for investigating how the epigenetic code influences cellular fate. High-throughput sequencing of these cell types has yielded DNA methylation maps at single-nucleotide resolution and many genome-wide chromatin maps. In parallel to epigenome mapping efforts, remarkable progress has been made in our ability to manipulate cell states; ectopic expression of transcription factors has been shown to override developmentally established epigenetic marks and to enable routine generation of induced pluripotent stem (iPS) cells. Despite these advances, many fundamental questions remain. The roles of epigenetic marks and, in particular, of epigenetic modifiers in development and in disease states are not well understood. Although iPS cells appear molecularly and functionally similar to embryonic stem cells, more genome-wide studies are needed to define the extent and functions of epigenetic remodeling during reprogramming.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Meissner Adoi
10.1038/nbt.1684subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1079-88issue
10eissn
1087-0156issn
1546-1696pii
nbt.1684journal_volume
28pub_type
杂志文章,评审abstract：:We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2052
更新日期：2011-11-27 00:00:00
abstract：:Most microbes in the biosphere remain unculturable. Whole genome shotgun (WGS) sequencing of environmental DNA (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. However, in communities of high complexity, metagenomics fails to link specific microbes to specific ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1488
更新日期：2008-09-01 00:00:00
abstract：:We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization d...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0396-303
更新日期：1996-03-01 00:00:00
abstract：:A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essentia...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt1324
更新日期：2007-08-01 00:00:00
abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/72671
更新日期：2000-02-01 00:00:00
abstract：:The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0412-8
更新日期：2020-04-01 00:00:00
abstract：:Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0606-643
更新日期：2006-06-01 00:00:00
abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4047
更新日期：2018-02-01 00:00:00
abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0067-5
更新日期：2019-04-01 00:00:00
abstract：:CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3437
更新日期：2016-02-01 00:00:00
abstract：:As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0506-517
更新日期：2006-05-01 00:00:00
abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0465-8
更新日期：2020-06-01 00:00:00
abstract：:Complex interactions among genetic components often result in variable systemic performance in designed multigene systems. Using the bacterial clustered regularly interspaced short palindromic repeat (CRISPR) pathway we develop a synthetic RNA-processing platform, and show that efficient and specific cleavage of precu...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2355
更新日期：2012-10-01 00:00:00
abstract：:We describe a novel approach to design a set of primers selective for large groups of genes. This method is based on the distribution frequency of all nucleotide combinations (octa- to decanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect genes. By analyzing 1000 human ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0796-857
更新日期：1996-07-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, rob...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1629
更新日期：2010-06-01 00:00:00
abstract：:Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1206-1504
更新日期：2006-12-01 00:00:00
abstract：:The Human Proteome Organization (HUPO) recently completed the first large-scale collaborative study to characterize the human serum and plasma proteomes. The study was carried out in different locations and used diverse methods and instruments to compare and integrate tandem mass spectrometry (MS/MS) data on aliquots ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1183
更新日期：2006-03-01 00:00:00
abstract：:Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1076
更新日期：2005-04-01 00:00:00
abstract：:Although bile duct disorders are well-recognized causes of liver disease, the molecular and cellular events leading to biliary dysfunction are poorly understood. To enable modeling and drug discovery for biliary disease, we describe a protocol that achieves efficient differentiation of biliary epithelial cells (cholan...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3294
更新日期：2015-08-01 00:00:00
abstract：:Correct reconstruction of macromolecular structure by cryo-electron microscopy (cryo-EM) relies on accurate determination of the orientation of single-particle images. For small (<100 kDa) DNA-binding proteins, obtaining particle images with sufficiently asymmetric features to correctly guide alignment is challenging....
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0716-8
更新日期：2020-10-19 00:00:00
abstract：:The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a kno...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt761
更新日期：2002-12-01 00:00:00
abstract：:We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1077
更新日期：2005-04-01 00:00:00
abstract：:We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, acti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/13732
更新日期：1999-10-01 00:00:00
abstract：:RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1541
更新日期：2009-06-01 00:00:00
abstract：:To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0598-431
更新日期：1998-05-01 00:00:00
abstract：:Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout t...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1289
更新日期：2007-03-01 00:00:00
abstract：:Pluripotent stem cell-derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal m...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4162
更新日期：2018-08-01 00:00:00
abstract：:A problem in analyzing microarray-based gene expression data is the separation of genes causally involved in a disease from innocent bystander genes, whose expression levels have been secondarily altered by primary changes elsewhere. To investigate this issue systematically in the context of a class of complex human d...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt965
更新日期：2004-05-01 00:00:00
abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...
journal_title：Nature biotechnology
pub_type： 评论,社论
doi：10.1038/nbt0909-777
更新日期：2009-09-01 00:00:00
abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1073
更新日期：2005-03-01 00:00:00
