Insights into US public biotech sector using patenting trends.


:Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies.


Nat Biotechnol


Nature biotechnology


Aggarwal S,Gupta V,Bagchi-Sen S




Has Abstract


2006-06-01 00:00:00














  • Functional interrogation and mining of natively paired human VH:VL antibody repertoires.

    abstract::We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

    更新日期:2018-02-01 00:00:00

  • Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.

    abstract::Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Hilton IB,D'Ippolito AM,Vockley CM,Thakore PI,Crawford GE,Reddy TE,Gersbach CA

    更新日期:2015-05-01 00:00:00

  • Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

    abstract::RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Kuscu C,Arslan S,Singh R,Thorpe J,Adli M

    更新日期:2014-07-01 00:00:00

  • Directed evolution of proteins by exon shuffling.

    abstract::Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审


    authors: Kolkman JA,Stemmer WP

    更新日期:2001-05-01 00:00:00

  • Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids.

    abstract::The sap-sucking insects (order Hemiptera), including aphids, planthoppers, whiteflies and stink bugs, present one of the greatest challenges for pest management in global agriculture. Insect neurotoxins offer an alternative to chemical insecticides for controlling these pests, but require delivery into the insect hemo...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Bonning BC,Pal N,Liu S,Wang Z,Sivakumar S,Dixon PM,King GF,Miller WA

    更新日期:2014-01-01 00:00:00

  • Spontaneous change of a benign satellite RNA of cucumber mosaic virus to a pathogenic variant.

    abstract::Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Palukaitis P,Roossinck MJ

    更新日期:1996-10-01 00:00:00

  • Safeguarding CRISPR-Cas9 gene drives in yeast.

    abstract::RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: DiCarlo JE,Chavez A,Dietz SL,Esvelt KM,Church GM

    更新日期:2015-12-01 00:00:00

  • RNAi modulation of placental sFLT1 for the treatment of preeclampsia.

    abstract::Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

    更新日期:2018-11-19 00:00:00

  • Protein detection using proximity-dependent DNA ligation assays.

    abstract::The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe....

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Fredriksson S,Gullberg M,Jarvius J,Olsson C,Pietras K,Gústafsdóttir SM,Ostman A,Landegren U

    更新日期:2002-05-01 00:00:00

  • A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.

    abstract::Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Kogure T,Karasawa S,Araki T,Saito K,Kinjo M,Miyawaki A

    更新日期:2006-05-01 00:00:00

  • Problems in monitoring horizontal gene transfer in field trials of transgenic plants.

    abstract::Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Heinemann JA,Traavik T

    更新日期:2004-09-01 00:00:00

  • StringTie enables improved reconstruction of a transcriptome from RNA-seq reads.

    abstract::Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Pertea M,Pertea GM,Antonescu CM,Chang TC,Mendell JT,Salzberg SL

    更新日期:2015-03-01 00:00:00

  • Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin.

    abstract::The side chain of the antifungal antibiotic ansatrienin A from Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC)-derived moiety. This moiety is also observed in trace amounts of omega-cyclohexyl fatty acids (typically less than 1% of total fatty acids) produced by S. collinus. Coenzyme A-activated CHC ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Cropp TA,Wilson DJ,Reynolds KA

    更新日期:2000-09-01 00:00:00

  • The chemical evolution of oligonucleotide therapies of clinical utility.

    abstract::After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审


    authors: Khvorova A,Watts JK

    更新日期:2017-03-01 00:00:00

  • Generation of oligodendroglial cells by direct lineage conversion.

    abstract::Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin. However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available. Here we report the generation of induced ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Yang N,Zuchero JB,Ahlenius H,Marro S,Ng YH,Vierbuchen T,Hawkins JS,Geissler R,Barres BA,Wernig M

    更新日期:2013-05-01 00:00:00

  • Large multiple sequence alignments with a root-to-leaf regressive method.

    abstract::Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Garriga E,Di Tommaso P,Magis C,Erb I,Mansouri L,Baltzis A,Laayouni H,Kondrashov F,Floden E,Notredame C

    更新日期:2019-12-01 00:00:00

  • Programmable m6A modification of cellular RNAs with a Cas13-directed methyltransferase.

    abstract::N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Wilson C,Chen PJ,Miao Z,Liu DR

    更新日期:2020-12-01 00:00:00

  • Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase.

    abstract::Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Richins RD,Kaneva I,Mulchandani A,Chen W

    更新日期:1997-10-01 00:00:00

  • Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content.

    abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Cahoon EB,Hall SE,Ripp KG,Ganzke TS,Hitz WD,Coughlan SJ

    更新日期:2003-09-01 00:00:00

  • Resequencing 50 accessions of cultivated and wild rice yields markers for identifying agronomically important genes.

    abstract::Rice is a staple crop that has undergone substantial phenotypic and physiological changes during domestication. Here we resequenced the genomes of 40 cultivated accessions selected from the major groups of rice and 10 accessions of their wild progenitors (Oryza rufipogon and Oryza nivara) to >15 × raw data coverage. W...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Xu X,Liu X,Ge S,Jensen JD,Hu F,Li X,Dong Y,Gutenkunst RN,Fang L,Huang L,Li J,He W,Zhang G,Zheng X,Zhang F,Li Y,Yu C,Kristiansen K,Zhang X,Wang J,Wright M,McCouch S,Nielsen R,Wang J,Wang W

    更新日期:2011-12-11 00:00:00

  • Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.

    abstract::Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, rob...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Melkoumian Z,Weber JL,Weber DM,Fadeev AG,Zhou Y,Dolley-Sonneville P,Yang J,Qiu L,Priest CA,Shogbon C,Martin AW,Nelson J,West P,Beltzer JP,Pal S,Brandenberger R

    更新日期:2010-06-01 00:00:00

  • Fully mobilizing host defense: building better vaccines.

    abstract::Developments in methods for identifying antigens from infectious agents and cancers has provided exciting new opportunities in prevention and treatment through vaccination. In many of these situations, however, traditional immunization techniques do not stimulate protective immunity because they fail to fully mobilize...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审


    authors: Raychaudhuri S,Rock KL

    更新日期:1998-11-01 00:00:00

  • Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas.

    abstract::Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Moncada R,Barkley D,Wagner F,Chiodin M,Devlin JC,Baron M,Hajdu CH,Simeone DM,Yanai I

    更新日期:2020-03-01 00:00:00

  • Mapping recently identified nucleotide variants in the genome and transcriptome.

    abstract::Nucleotide variants, especially those related to epigenetic functions, provide critical regulatory information beyond simple genomic sequence, and they define cell status in higher organisms. 5-Methylcytosine, which is found in DNA, was until recently the only nucleotide variant studied in terms of epigenetics in euka...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审


    authors: Song CX,Yi C,He C

    更新日期:2012-11-01 00:00:00

  • Resolution of cutaneous inflammation after local elimination of macrophages.

    abstract::We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Thepen T,van Vuuren AJ,Kiekens RC,Damen CA,Vooijs WC,van De Winkel JG

    更新日期:2000-01-01 00:00:00

  • Excision of selectable marker genes from transgenic plants.

    abstract::Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章,评审


    authors: Hare PD,Chua NH

    更新日期:2002-06-01 00:00:00

  • A catalog of the mouse gut metagenome.

    abstract::We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

    更新日期:2015-10-01 00:00:00

  • Evolutionary molecular engineering by random elongation mutagenesis.

    abstract::We describe a new method of random mutagenesis that employs the addition of peptide tails with random sequences to the C-terminal of enzyme molecules. A mutant population of catalase I from Bacillus stearothermophilus prepared by this method has a diversity in thermostability and enzyme activity equal to that obtained...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Matsuura T,Miyai K,Trakulnaleamsai S,Yomo T,Shima Y,Miki S,Yamamoto K,Urabe I

    更新日期:1999-01-01 00:00:00

  • High-throughput genome scaffolding from in vivo DNA interaction frequency.

    abstract::Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Kaplan N,Dekker J

    更新日期:2013-12-01 00:00:00

  • Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy.

    abstract::A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....

    journal_title:Nature biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Bai L,Cong L,Yu P,Zhang T,Shi W,Li F,Du J,Wang K

    更新日期:2021-01-01 00:00:00