Abstract:
:Complex interactions among genetic components often result in variable systemic performance in designed multigene systems. Using the bacterial clustered regularly interspaced short palindromic repeat (CRISPR) pathway we develop a synthetic RNA-processing platform, and show that efficient and specific cleavage of precursor mRNA enables reliable and predictable regulation of multigene operons. Physical separation of linked genetic elements by CRISPR-mediated cleavage is an effective strategy to achieve assembly of promoters, ribosome binding sites, cis-regulatory elements, and riboregulators into single- and multigene operons with predictable functions in bacteria. We also demonstrate that CRISPR-based RNA cleavage is effective for regulation in bacteria, archaea and eukaryotes. Programmable RNA processing using CRISPR offers a general approach for creating context-free genetic elements and can be readily used in the bottom-up construction of increasingly complex biological systems in a plug-and-play manner.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Qi L,Haurwitz RE,Shao W,Doudna JA,Arkin APdoi
10.1038/nbt.2355subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1002-6issue
10eissn
1087-0156issn
1546-1696pii
nbt.2355journal_volume
30pub_type
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