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Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator.

Abstract：

:Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II)2Cys6-encoding genes improved partitioning of total carbon to lipids from 20% (wild type) to 40-55% (mutant) in nutrient-replete conditions. Knockout mutants grew poorly, but attenuation of Zn(II)2Cys6 expression yielded strains producing twice as much lipid (∼5.0 g m-2 d-1) as that in the wild type (∼2.5 g m-2 d-1) under semicontinuous growth conditions and had little effect on growth.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Ajjawi I,Verruto J,Aqui M,Soriaga LB,Coppersmith J,Kwok K,Peach L,Orchard E,Kalb R,Xu W,Carlson TJ,Francis K,Konigsfeld K,Bartalis J,Schultz A,Lambert W,Schwartz AS,Brown R,Moellering ER

doi

10.1038/nbt.3865

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

647-652

issue

7

eissn

1087-0156

issn

1546-1696

pii

nbt.3865

journal_volume

35

pub_type

杂志文章

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