An electrical probe of protein-DNA interactions on DNA-modified surfaces.

abstract：:Rice is a staple crop that has undergone substantial phenotypic and physiological changes during domestication. Here we resequenced the genomes of 40 cultivated accessions selected from the major groups of rice and 10 accessions of their wild progenitors (Oryza rufipogon and Oryza nivara) to >15 × raw data coverage. W...

journal_title：Nature biotechnology

authors： Xu X,Liu X,Ge S,Jensen JD,Hu F,Li X,Dong Y,Gutenkunst RN,Fang L,Huang L,Li J,He W,Zhang G,Zheng X,Zhang F,Li Y,Yu C,Kristiansen K,Zhang X,Wang J,Wright M,McCouch S,Nielsen R,Wang J,Wang W

更新日期：2011-12-11 00:00:00

abstract：:Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...

journal_title：Nature biotechnology

authors： Khetani SR,Bhatia SN

更新日期：2008-01-01 00:00:00

abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...

journal_title：Nature biotechnology

authors： Cheung AS,Zhang DKY,Koshy ST,Mooney DJ

更新日期：2018-02-01 00:00:00

abstract：:A variety of strategies to incorporate unnatural amino acids into proteins have been pursued, but all have limitations with respect to technical accessibility, scalability, applicability to in vivo studies, or site specificity of amino acid incorporation. The ability to selectively introduce unnatural functional group...

journal_title：Nature biotechnology

authors： Santoro SW,Wang L,Herberich B,King DS,Schultz PG

更新日期：2002-10-01 00:00:00

abstract：:Developments in methods for identifying antigens from infectious agents and cancers has provided exciting new opportunities in prevention and treatment through vaccination. In many of these situations, however, traditional immunization techniques do not stimulate protective immunity because they fail to fully mobilize...

journal_title：Nature biotechnology

authors： Raychaudhuri S,Rock KL

更新日期：1998-11-01 00:00:00

abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...

journal_title：Nature biotechnology

authors： Deng J,Shoemaker R,Xie B,Gore A,LeProust EM,Antosiewicz-Bourget J,Egli D,Maherali N,Park IH,Yu J,Daley GQ,Eggan K,Hochedlinger K,Thomson J,Wang W,Gao Y,Zhang K

更新日期：2009-04-01 00:00:00

abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...

journal_title：Nature biotechnology

authors： Schafer AJ,Hawkins JR

更新日期：1998-01-01 00:00:00

abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...

journal_title：Nature biotechnology

authors： Hoebe RA,Van Oven CH,Gadella TW Jr,Dhonukshe PB,Van Noorden CJ,Manders EM

更新日期：2007-02-01 00:00:00

abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...

journal_title：Nature biotechnology

authors： Kalhor R,Tjong H,Jayathilaka N,Alber F,Chen L

更新日期：2011-12-25 00:00:00

abstract：:Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...

journal_title：Nature biotechnology

authors： Zhao D,Li J,Li S,Xin X,Hu M,Price MA,Rosser SJ,Bi C,Zhang X

更新日期：2021-01-01 00:00:00

abstract：:St. Louis wants to become a hub of agricultural biotechnology. All it needs is more start-ups and funds. ...

journal_title：Nature biotechnology

authors： Marris E

更新日期：2008-04-01 00:00:00

abstract：:Pluripotent stem cell-derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal m...

journal_title：Nature biotechnology

authors： Liu YW,Chen B,Yang X,Fugate JA,Kalucki FA,Futakuchi-Tsuchida A,Couture L,Vogel KW,Astley CA,Baldessari A,Ogle J,Don CW,Steinberg ZL,Seslar SP,Tuck SA,Tsuchida H,Naumova AV,Dupras SK,Lyu MS,Lee J,Hailey DW,Reinec

更新日期：2018-08-01 00:00:00

abstract：:Engineered T cells are effective therapies against a range of malignancies, but current approaches rely on autologous T cells, which are difficult and expensive to manufacture. Efforts to develop potent allogeneic T cells that are not rejected by the recipient's immune system require abrogating both T- and natural kil...

journal_title：Nature biotechnology

authors： Mo F,Watanabe N,McKenna MK,Hicks MJ,Srinivasan M,Gomes-Silva D,Atilla E,Smith T,Ataca Atilla P,Ma R,Quach D,Heslop HE,Brenner MK,Mamonkin M

更新日期：2021-01-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Ramsköld D,Luo S,Wang YC,Li R,Deng Q,Faridani OR,Daniels GA,Khrebtukova I,Loring JF,Laurent LC,Schroth GP,Sandberg R

更新日期：2020-03-01 00:00:00

abstract：:Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved...

journal_title：Nature biotechnology

authors： Van der Verren SE,Van Gerven N,Jonckheere W,Hambley R,Singh P,Kilgour J,Jordan M,Wallace EJ,Jayasinghe L,Remaut H

更新日期：2020-12-01 00:00:00

abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...

journal_title：Nature biotechnology

authors： Ochoa D,Jarnuczak AF,Viéitez C,Gehre M,Soucheray M,Mateus A,Kleefeldt AA,Hill A,Garcia-Alonso L,Stein F,Krogan NJ,Savitski MM,Swaney DL,Vizcaíno JA,Noh KM,Beltrao P

更新日期：2020-03-01 00:00:00

abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...

journal_title：Nature biotechnology

authors： Kaplan N,Dekker J

更新日期：2013-12-01 00:00:00

abstract：:Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...

journal_title：Nature biotechnology

authors： Pertea M,Pertea GM,Antonescu CM,Chang TC,Mendell JT,Salzberg SL

更新日期：2015-03-01 00:00:00

abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...

journal_title：Nature biotechnology

authors： Eisenstein M

更新日期：2011-02-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...

journal_title：Nature biotechnology

authors： Moncada R,Barkley D,Wagner F,Chiodin M,Devlin JC,Baron M,Hajdu CH,Simeone DM,Yanai I

更新日期：2020-03-01 00:00:00

abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

journal_title：Nature biotechnology

authors： Bzikadze AV,Pevzner PA

更新日期：2020-11-01 00:00:00

abstract：:Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

journal_title：Nature biotechnology

authors： Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

更新日期：2018-11-19 00:00:00

abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...

journal_title：Nature biotechnology

authors： Hosaka T,Ohnishi-Kameyama M,Muramatsu H,Murakami K,Tsurumi Y,Kodani S,Yoshida M,Fujie A,Ochi K

更新日期：2009-05-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

journal_title：Nature biotechnology

authors： Khvorova A,Watts JK

更新日期：2017-03-01 00:00:00

abstract：:Functional genomics and proteomics are identifying many potential drug targets for novel therapeutic proteins, and both rational and combinatorial protein engineering methods are available for creating drug candidates. A central challenge is the definition of the most appropriate design criteria, which will benefit cr...

journal_title：Nature biotechnology

authors： Rao BM,Lauffenburger DA,Wittrup KD

更新日期：2005-02-01 00:00:00

abstract：:Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient exp...

journal_title：Nature biotechnology

authors： Mercer TR,Gerhardt DJ,Dinger ME,Crawford J,Trapnell C,Jeddeloh JA,Mattick JS,Rinn JL

更新日期：2011-11-13 00:00:00

abstract：:DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B...

journal_title：Nature biotechnology

authors： Savelyeva N,Munday R,Spellerberg MB,Lomonossoff GP,Stevenson FK

更新日期：2001-08-01 00:00:00

abstract：:Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...

journal_title：Nature biotechnology

authors： Chen W,Zhao Y,Chen X,Yang Z,Xu X,Bi Y,Chen V,Li J,Choi H,Ernest B,Tran B,Mehta M,Kumar P,Farmer A,Mir A,Mehra UA,Li JL,Moos M Jr,Xiao W,Wang C

更新日期：2020-12-21 00:00:00

abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

journal_title：Nature biotechnology

authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

更新日期：2012-12-01 00:00:00