Abstract:
:DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B-cell tumors, encoded by the variable region genes, are clone-specific tumor antigens. When assembled as single-chain Fv (scFv) alone in a DNA vaccine, immunogenicity is low. Previously, we found that fusion of a sequence from tetanus toxin (fragment C; FrC) promoted anti-idiotypic protection against lymphoma and myeloma. We have now investigated an alternative fusion gene derived from a plant virus, potato virus X coat protein, a primary antigen in humans. When fused to scFv, the self-aggregating protein generates protection against lymphoma and myeloma. In contrast to scFv-FrC, protection against lymphoma is mediated by CD4+ T cells, as is protection against myeloma. Plant viral proteins offer new opportunities to activate immunity against linked T-cell epitopes to attack cancer.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Savelyeva N,Munday R,Spellerberg MB,Lomonossoff GP,Stevenson FKdoi
10.1038/90816subject
Has Abstractpub_date
2001-08-01 00:00:00pages
760-4issue
8eissn
1087-0156issn
1546-1696pii
90816journal_volume
19pub_type
杂志文章abstract::Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4297
更新日期:2018-11-19 00:00:00
abstract::An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0508-519
更新日期:2008-05-01 00:00:00
abstract::Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0322-9
更新日期:2020-02-01 00:00:00
abstract::Numerous biological processes involve the recognition of a specific pattern of binding sites on a target protein or surface. Although ligands displayed by disordered scaffolds form stochastic rather than specific patterns, theoretical models predict that recognition will occur between patterns that are characterized b...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1204
更新日期:2006-05-01 00:00:00
abstract::Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0398-281
更新日期:1998-03-01 00:00:00
abstract::Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1275
更新日期:2007-01-01 00:00:00
abstract::Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2768
更新日期:2013-12-01 00:00:00
abstract::The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1641
更新日期:2010-06-01 00:00:00
abstract::Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4047
更新日期:2018-02-01 00:00:00
abstract::Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/82414
更新日期:2000-12-01 00:00:00
abstract::Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient exp...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2024
更新日期:2011-11-13 00:00:00
abstract::We have evaluated inhibition of the plasmid-born chloramphenicol acetyl transferase gene (CAT) by the hammerhead ribozyme and antisense RNA in Escherichia coli where the translation and transcription rates have been modified. Whereas neither antisense nor the hammerhead had an inhibitory effect on CAT activity in wild...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0597-432
更新日期:1997-05-01 00:00:00
abstract::Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt986
更新日期:2004-07-01 00:00:00
abstract::After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.3765
更新日期:2017-03-01 00:00:00
abstract::The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0197-9
更新日期:2019-08-01 00:00:00
abstract::Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strai...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2858
更新日期:2014-04-01 00:00:00
abstract::Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural' natural products but existing techniques for this are time consuming...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1128
更新日期:2005-09-01 00:00:00
abstract::Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3350
更新日期:2015-10-01 00:00:00
abstract::Epigenetic modifications constitute a complex regulatory layer on top of the genome sequence. Pluripotent and differentiated cells provide a powerful system for investigating how the epigenetic code influences cellular fate. High-throughput sequencing of these cell types has yielded DNA methylation maps at single-nucl...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.1684
更新日期:2010-10-01 00:00:00
abstract::MicroRNAs (miRNAs) are increasingly implicated in the regulation of metastasis. Despite their potential as targets for anti-metastatic therapy, miRNAs have only been silenced in normal tissues of rodents and nonhuman primates. Therefore, the development of effective approaches for sequence-specific inhibition of miRNA...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1618
更新日期:2010-04-01 00:00:00
abstract::Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0592-2
更新日期:2021-01-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0658-1
更新日期:2020-09-01 00:00:00
abstract::Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3208
更新日期:2015-05-01 00:00:00
abstract::Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1170
更新日期:2006-01-01 00:00:00
abstract::We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1538
更新日期:2009-05-01 00:00:00
abstract::N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0572-6
更新日期:2020-12-01 00:00:00
abstract::Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.4022
更新日期:2017-12-01 00:00:00
abstract::Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1361
更新日期:2008-01-01 00:00:00
abstract::High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt836
更新日期:2003-07-01 00:00:00
abstract::Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1207
更新日期:2006-05-01 00:00:00