In vitro selection of signaling aptamers.

abstract：:The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single ce...

journal_title：Nature biotechnology

authors： Lan F,Demaree B,Ahmed N,Abate AR

更新日期：2017-07-01 00:00:00

abstract：:Biological systems are controlled by protein complexes that associate into dynamic protein interaction networks. We describe a strategy that analyzes protein complexes through the integration of label-free, quantitative mass spectrometry and computational analysis. By evaluating peptide intensity profiles throughout t...

journal_title：Nature biotechnology

authors： Rinner O,Mueller LN,Hubálek M,Müller M,Gstaiger M,Aebersold R

更新日期：2007-03-01 00:00:00

abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...

journal_title：Nature biotechnology

authors： Li M,Chen WD,Papadopoulos N,Goodman SN,Bjerregaard NC,Laurberg S,Levin B,Juhl H,Arber N,Moinova H,Durkee K,Schmidt K,He Y,Diehl F,Velculescu VE,Zhou S,Diaz LA Jr,Kinzler KW,Markowitz SD,Vogelstein B

更新日期：2009-09-01 00:00:00

abstract：:De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly, we present Shasta, a de novo long-read assembler, and polishing algorithms named MarginPolish and HELEN. Using a single P...

journal_title：Nature biotechnology

authors： Shafin K,Pesout T,Lorig-Roach R,Haukness M,Olsen HE,Bosworth C,Armstrong J,Tigyi K,Maurer N,Koren S,Sedlazeck FJ,Marschall T,Mayes S,Costa V,Zook JM,Liu KJ,Kilburn D,Sorensen M,Munson KM,Vollger MR,Monlong J,Gar

更新日期：2020-09-01 00:00:00

abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...

journal_title：Nature biotechnology

authors：

更新日期：2009-09-01 00:00:00

abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...

journal_title：Nature biotechnology

authors： Perez JT,Pham AM,Lorini MH,Chua MA,Steel J,tenOever BR

更新日期：2009-06-01 00:00:00

abstract：:The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...

journal_title：Nature biotechnology

authors： Rissin DM,Kan CW,Campbell TG,Howes SC,Fournier DR,Song L,Piech T,Patel PP,Chang L,Rivnak AJ,Ferrell EP,Randall JD,Provuncher GK,Walt DR,Duffy DC

更新日期：2010-06-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cu...

journal_title：Nature biotechnology

authors： Altpeter F,Vasil V,Srivastava V,Vasil IK

更新日期：1996-09-01 00:00:00

abstract：:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...

journal_title：Nature biotechnology

authors： Haasnoot J,Westerhout EM,Berkhout B

更新日期：2007-12-01 00:00:00

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...

journal_title：Nature biotechnology

authors： Thepen T,van Vuuren AJ,Kiekens RC,Damen CA,Vooijs WC,van De Winkel JG

更新日期：2000-01-01 00:00:00

abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...

journal_title：Nature biotechnology

authors： Xiang S,Fruehauf J,Li CJ

更新日期：2006-06-01 00:00:00

abstract：:Efficient delivery of small interfering (si)RNA to specific cell populations in vivo remains a formidable challenge to its successful therapeutic application. We show that siRNA synthetically linked to a CpG oligonucleotide agonist of toll-like receptor (TLR)9 targets and silences genes in TLR9(+) myeloid cells and B ...

journal_title：Nature biotechnology

authors： Kortylewski M,Swiderski P,Herrmann A,Wang L,Kowolik C,Kujawski M,Lee H,Scuto A,Liu Y,Yang C,Deng J,Soifer HS,Raubitschek A,Forman S,Rossi JJ,Pardoll DM,Jove R,Yu H

更新日期：2009-10-01 00:00:00

abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...

journal_title：Nature biotechnology

authors： Zhu Q,Shah S,Dries R,Cai L,Yuan GC

更新日期：2018-10-29 00:00:00

abstract：:Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase transition occurs, leading to aggregation of the polypeptide. We demonstrate a method for purification of soluble fusio...

journal_title：Nature biotechnology

authors： Meyer DE,Chilkoti A

更新日期：1999-11-01 00:00:00

abstract：:Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...

journal_title：Nature biotechnology

authors： Liu Y,Mi Y,Mueller T,Kreibich S,Williams EG,Van Drogen A,Borel C,Frank M,Germain PL,Bludau I,Mehnert M,Seifert M,Emmenlauer M,Sorg I,Bezrukov F,Bena FS,Zhou H,Dehio C,Testa G,Saez-Rodriguez J,Antonarakis SE,Hard

更新日期：2019-03-01 00:00:00

abstract：:Nucleotide variants, especially those related to epigenetic functions, provide critical regulatory information beyond simple genomic sequence, and they define cell status in higher organisms. 5-Methylcytosine, which is found in DNA, was until recently the only nucleotide variant studied in terms of epigenetics in euka...

journal_title：Nature biotechnology

authors： Song CX,Yi C,He C

更新日期：2012-11-01 00:00:00

abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

journal_title：Nature biotechnology

authors： Kolkman JA,Stemmer WP

更新日期：2001-05-01 00:00:00

abstract：:This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...

journal_title：Nature biotechnology

authors： Cesaro-Tadic S,Lagos D,Honegger A,Rickard JH,Partridge LJ,Blackburn GM,Plückthun A

更新日期：2003-06-01 00:00:00

abstract：:Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AA...

journal_title：Nature biotechnology

authors： Deverman BE,Pravdo PL,Simpson BP,Kumar SR,Chan KY,Banerjee A,Wu WL,Yang B,Huber N,Pasca SP,Gradinaru V

更新日期：2016-02-01 00:00:00

abstract：:Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crys...

journal_title：Nature biotechnology

authors： Moellenbeck DJ,Peters ML,Bing JW,Rouse JR,Higgins LS,Sims L,Nevshemal T,Marshall L,Ellis RT,Bystrak PG,Lang BA,Stewart JL,Kouba K,Sondag V,Gustafson V,Nour K,Xu D,Swenson J,Zhang J,Czapla T,Schwab G,Jayne S,St

更新日期：2001-07-01 00:00:00

abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...

journal_title：Nature biotechnology

authors： Mallick P,Schirle M,Chen SS,Flory MR,Lee H,Martin D,Ranish J,Raught B,Schmitt R,Werner T,Kuster B,Aebersold R

更新日期：2007-01-01 00:00:00

abstract：:Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...

journal_title：Nature biotechnology

authors： Jeck WR,Sharpless NE

更新日期：2014-05-01 00:00:00

abstract：:Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...

journal_title：Nature biotechnology

authors： Sönnichsen C,Reinhard BM,Liphardt J,Alivisatos AP

更新日期：2005-06-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

更新日期：2019-11-01 00:00:00

abstract：:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...

journal_title：Nature biotechnology

authors： Hare PD,Chua NH

更新日期：2002-06-01 00:00:00

abstract：:The re-use of previously validated designs is critical to the evolution of synthetic biology from a research discipline to an engineering practice. Here we describe the Synthetic Biology Open Language (SBOL), a proposed data standard for exchanging designs within the synthetic biology community. SBOL represents synthe...

journal_title：Nature biotechnology

authors： Galdzicki M,Clancy KP,Oberortner E,Pocock M,Quinn JY,Rodriguez CA,Roehner N,Wilson ML,Adam L,Anderson JC,Bartley BA,Beal J,Chandran D,Chen J,Densmore D,Endy D,Grünberg R,Hallinan J,Hillson NJ,Johnson JD,Kuchinsky

更新日期：2014-06-01 00:00:00

abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...

journal_title：Nature biotechnology

authors： Brenner S,Johnson M,Bridgham J,Golda G,Lloyd DH,Johnson D,Luo S,McCurdy S,Foy M,Ewan M,Roth R,George D,Eletr S,Albrecht G,Vermaas E,Williams SR,Moon K,Burcham T,Pallas M,DuBridge RB,Kirchner J,Fearon K,Mao J,

更新日期：2000-06-01 00:00:00

abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

journal_title：Nature biotechnology

authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

更新日期：2012-12-01 00:00:00