Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase.

abstract：:Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...

journal_title：Nature biotechnology

authors： Aggarwal S,Gupta V,Bagchi-Sen S

更新日期：2006-06-01 00:00:00

abstract：:Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...

journal_title：Nature biotechnology

authors： Yarema KJ,Goon S,Bertozzi CR

更新日期：2001-06-01 00:00:00

abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...

journal_title：Nature biotechnology

authors： Cheung AS,Zhang DKY,Koshy ST,Mooney DJ

更新日期：2018-02-01 00:00:00

abstract：:Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

journal_title：Nature biotechnology

authors： Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

更新日期：2018-11-19 00:00:00

abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...

journal_title：Nature biotechnology

authors： Ochoa D,Jarnuczak AF,Viéitez C,Gehre M,Soucheray M,Mateus A,Kleefeldt AA,Hill A,Garcia-Alonso L,Stein F,Krogan NJ,Savitski MM,Swaney DL,Vizcaíno JA,Noh KM,Beltrao P

更新日期：2020-03-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:For the past five decades, the need for new antibiotics has been met largely by semisynthetic tailoring of natural product scaffolds discovered in the middle of the 20(th) century. More recently, however, advances in technology have sparked a resurgence in the discovery of natural product antibiotics from bacterial so...

journal_title：Nature biotechnology

authors： Clardy J,Fischbach MA,Walsh CT

更新日期：2006-12-01 00:00:00

abstract：:Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...

journal_title：Nature biotechnology

authors： Jeck WR,Sharpless NE

更新日期：2014-05-01 00:00:00

abstract：:Reagentless biosensors that can directly transduce molecular recognition to optical signals should potentiate the development of sensor arrays for a wide variety of analytes. Nucleic acid aptamers that bind ligands tightly and specifically can be readily selected, but may prove difficult to adapt to biosensor applicat...

journal_title：Nature biotechnology

authors： Jhaveri S,Rajendran M,Ellington AD

更新日期：2000-12-01 00:00:00

abstract：:Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...

journal_title：Nature biotechnology

authors： Faria M,Spiller DG,Dubertret C,Nelson JS,White MR,Scherman D,Hélène C,Giovannangeli C

更新日期：2001-01-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...

journal_title：Nature biotechnology

authors： Ishizaki-Nishizawa O,Fujii T,Azuma M,Sekiguchi K,Murata N,Ohtani T,Toguri T

更新日期：1996-08-01 00:00:00

abstract：:Genetic testing for rare heart conditions might someday expand to more common cardiac ailments. Already there are signs testing is dramatically changing how some conditions are treated and doctors' definition of who a patient is. Stephen Strauss reports. ...

journal_title：Nature biotechnology

authors： Strauss S

更新日期：2010-10-01 00:00:00

abstract：:The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial therapy. Metagenomic sequencing could identify LRI pathogens much faster than culture, but methods are needed to remove the large amoun...

journal_title：Nature biotechnology

authors： Charalampous T,Kay GL,Richardson H,Aydin A,Baldan R,Jeanes C,Rae D,Grundy S,Turner DJ,Wain J,Leggett RM,Livermore DM,O'Grady J

更新日期：2019-07-01 00:00:00

abstract：:Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...

journal_title：Nature biotechnology

authors： Chick JM,Kolippakkam D,Nusinow DP,Zhai B,Rad R,Huttlin EL,Gygi SP

更新日期：2015-07-01 00:00:00

abstract：:The re-use of previously validated designs is critical to the evolution of synthetic biology from a research discipline to an engineering practice. Here we describe the Synthetic Biology Open Language (SBOL), a proposed data standard for exchanging designs within the synthetic biology community. SBOL represents synthe...

journal_title：Nature biotechnology

authors： Galdzicki M,Clancy KP,Oberortner E,Pocock M,Quinn JY,Rodriguez CA,Roehner N,Wilson ML,Adam L,Anderson JC,Bartley BA,Beal J,Chandran D,Chen J,Densmore D,Endy D,Grünberg R,Hallinan J,Hillson NJ,Johnson JD,Kuchinsky

更新日期：2014-06-01 00:00:00

abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...

journal_title：Nature biotechnology

authors： Schafer AJ,Hawkins JR

更新日期：1998-01-01 00:00:00

abstract：:Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...

journal_title：Nature biotechnology

authors： Montini E,Cesana D,Schmidt M,Sanvito F,Ponzoni M,Bartholomae C,Sergi Sergi L,Benedicenti F,Ambrosi A,Di Serio C,Doglioni C,von Kalle C,Naldini L

更新日期：2006-06-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Lam AT,Ma J,Barr C,Lee SA,White AK,Yu K,Riedel-Kruse IH

更新日期：2019-12-01 00:00:00

abstract：:Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...

journal_title：Nature biotechnology

authors： Richards M,Fong CY,Chan WK,Wong PC,Bongso A

更新日期：2002-09-01 00:00:00

abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...

journal_title：Nature biotechnology

authors： Nakai H,Storm TA,Kay MA

更新日期：2000-05-01 00:00:00

abstract：:The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe....

journal_title：Nature biotechnology

authors： Fredriksson S,Gullberg M,Jarvius J,Olsson C,Pietras K,Gústafsdóttir SM,Ostman A,Landegren U

更新日期：2002-05-01 00:00:00

abstract：:Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...

journal_title：Nature biotechnology

authors： Kogure T,Karasawa S,Araki T,Saito K,Kinjo M,Miyawaki A

更新日期：2006-05-01 00:00:00

abstract：:We present two algorithms to predict the activity of AsCpf1 guide RNAs. Indel frequencies for 15,000 target sequences were used in a deep-learning framework based on a convolutional neural network to train Seq-deepCpf1. We then incorporated chromatin accessibility information to create the better-performing DeepCpf1 a...

journal_title：Nature biotechnology

authors： Kim HK,Min S,Song M,Jung S,Choi JW,Kim Y,Lee S,Yoon S,Kim HH

更新日期：2018-03-01 00:00:00

abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...

journal_title：Nature biotechnology

authors： Furlong EE,Profitt D,Scott MP

更新日期：2001-02-01 00:00:00

abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...

journal_title：Nature biotechnology

authors： Segal E,Sirlin CB,Ooi C,Adler AS,Gollub J,Chen X,Chan BK,Matcuk GR,Barry CT,Chang HY,Kuo MD

更新日期：2007-06-01 00:00:00

abstract：:Cells isolated from the embryonic, neonatal, and adult rodent central nervous system divide in response to epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2), while retaining the ability to differentiate into neurons and glia. These cultures can be grown in aggregates termed neurospheres, which conta...

journal_title：Nature biotechnology

authors： Caldwell MA,He X,Wilkie N,Pollack S,Marshall G,Wafford KA,Svendsen CN

更新日期：2001-05-01 00:00:00

abstract：:While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent of designer biocatalysts, produced by informed selection and mutation through recombinant DNA tec...

journal_title：Nature biotechnology

authors： Burton SG,Cowan DA,Woodley JM

更新日期：2002-01-01 00:00:00

abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...

journal_title：Nature biotechnology

authors： Xiang S,Fruehauf J,Li CJ

更新日期：2006-06-01 00:00:00

abstract：:The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...

journal_title：Nature biotechnology

authors： Taipale M,Krykbaeva I,Whitesell L,Santagata S,Zhang J,Liu Q,Gray NS,Lindquist S

更新日期：2013-07-01 00:00:00