Abstract:
:The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe. The ligation of two such proximity probes gives rise to an amplifiable DNA sequence that reflects the identity and amount of the target protein. This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Fredriksson S,Gullberg M,Jarvius J,Olsson C,Pietras K,Gústafsdóttir SM,Ostman A,Landegren Udoi
10.1038/nbt0502-473subject
Has Abstractpub_date
2002-05-01 00:00:00pages
473-7issue
5eissn
1087-0156issn
1546-1696pii
nbt0502-473journal_volume
20pub_type
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