Abstract：
:Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximately 10 nm. Noble metal nanoparticles have plasmon resonances in the visible range and do not blink or bleach. They have been employed as alternative probes to overcome the limitations of organic fluorophores, and the coupling of plasmons in nearby particles has been exploited to detect particle aggregation by a distinct color change in bulk experiments. Here we demonstrate that plasmon coupling can be used to monitor distances between single pairs of gold and silver nanoparticles. We followed the directed assembly of gold and silver nanoparticle dimers in real time and studied the kinetics of single DNA hybridization events. These "plasmon rulers" allowed us to continuously monitor separations of up to 70 nm for >3,000 s.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Sönnichsen C,Reinhard BM,Liphardt J,Alivisatos APdoi
10.1038/nbt1100subject
Has Abstractpub_date
2005-06-01 00:00:00pages
741-5issue
6eissn
1087-0156issn
1546-1696pii
nbt1100journal_volume
23pub_type
杂志文章abstract：:Excessive and prolonged activity of inflammatory monocytes is a hallmark of many diseases with an inflammatory component. In such conditions, precise targeting of these cells could be therapeutically beneficial while sparing many essential functions of the innate immune system, thus limiting unwanted effects. Inflamma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1989
更新日期：2011-10-09 00:00:00
abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/7952
更新日期：1999-04-01 00:00:00
abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0260-6
更新日期：2019-11-01 00:00:00
abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0322-9
更新日期：2020-02-01 00:00:00
abstract：:Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4201
更新日期：2018-10-01 00:00:00
abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3585
更新日期：2016-08-01 00:00:00
abstract：:Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, ...
journal_title：Nature biotechnology
pub_type： 信件
doi：10.1038/nbt1093
更新日期：2005-05-01 00:00:00
abstract：:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0298-196
更新日期：1998-02-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0253-5
更新日期：2019-09-01 00:00:00
abstract：:While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent of designer biocatalysts, produced by informed selection and mutation through recombinant DNA tec...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0102-37
更新日期：2002-01-01 00:00:00
abstract：:Protein phosphorylation is a dominant mechanism of information transfer in cells, and a major goal of current proteomic efforts is to generate a system-level map describing all the sites of protein phosphorylation. Recent efforts have focused on developing technologies for enriching and quantifying phosphopeptides. Id...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt863
更新日期：2003-09-01 00:00:00
abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0797-663
更新日期：1997-07-01 00:00:00
abstract：:Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0178-z
更新日期：2019-09-01 00:00:00
abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3902
更新日期：2017-09-01 00:00:00
abstract：:Targeting of secreted and cell-surface proteins to the cell membrane is mediated by a short hydrophobic stretch of amino acids, termed the signal sequence. We have developed a method that detects signal sequences in cDNA fragments based on their ability to redirect a constitutively active mutant of a cytokine receptor...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/8666
更新日期：1999-05-01 00:00:00
abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0897-759
更新日期：1997-08-01 00:00:00
abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0497-369
更新日期：1997-04-01 00:00:00
abstract：:Replicate mass spectrometry (MS) measurements and the use of multiple analytical methods can greatly expand the comprehensiveness of shotgun proteomic profiling of biological samples. However, the inherent biases and variations in such data create computational and statistical challenges for quantitative comparative a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1592
更新日期：2010-01-01 00:00:00
abstract：:The density and long-term stability of DNA make it an appealing storage medium, particularly for long-term data archiving. Existing DNA storage technologies involve the synthesis and sequencing of multiple nominally identical molecules in parallel, resulting in information redundancy. We report the development of enco...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0240-x
更新日期：2019-10-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt792
更新日期：2003-03-01 00:00:00
abstract：:The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt802
更新日期：2003-04-01 00:00:00
abstract：:Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/83489
更新日期：2001-01-01 00:00:00
abstract：:Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strai...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2858
更新日期：2014-04-01 00:00:00
abstract：:RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt1369
更新日期：2007-12-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41587-020-0457-8
更新日期：2020-03-01 00:00:00
abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3199
更新日期：2015-05-01 00:00:00
abstract：:RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1541
更新日期：2009-06-01 00:00:00
abstract：:Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0398-281
更新日期：1998-03-01 00:00:00
abstract：:To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine beta- and kappa-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resul...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt783
更新日期：2003-02-01 00:00:00
abstract：:A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1145
更新日期：2005-10-01 00:00:00
