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Targeted isolation and cultivation of uncultivated bacteria by reverse genomics.

Abstract：

:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidated. Here, we report an approach to capture specific microorganisms from complex communities into pure cultures using genome-informed antibody engineering. We apply our reverse genomics approach to isolate and sequence single cells and to cultivate three different species-level lineages of human oral Saccharibacteria (TM7). Using our pure cultures, we show that all three Saccharibacteria species are epibionts of diverse Actinobacteria. We also isolate and cultivate human oral SR1 bacteria, which are members of a lineage of previously uncultured bacteria. Reverse-genomics-enabled cultivation of microorganisms can be applied to any species from any environment and has the potential to unlock the isolation, cultivation and characterization of species from as-yet-uncultured branches of the microbial tree of life.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Cross KL,Campbell JH,Balachandran M,Campbell AG,Cooper SJ,Griffen A,Heaton M,Joshi S,Klingeman D,Leys E,Yang Z,Parks JM,Podar M

doi

10.1038/s41587-019-0260-6

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

1314-1321

issue

11

eissn

1087-0156

issn

1546-1696

pii

10.1038/s41587-019-0260-6

journal_volume

37

pub_type

杂志文章

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