Abstract：
:We have evaluated inhibition of the plasmid-born chloramphenicol acetyl transferase gene (CAT) by the hammerhead ribozyme and antisense RNA in Escherichia coli where the translation and transcription rates have been modified. Whereas neither antisense nor the hammerhead had an inhibitory effect on CAT activity in wild-type E. coli, both reduced the level of the messenger RNA and the activity of the CAT gene by almost 60% in a slow ribosome mutant. Streptomycin, which increases the speed of translation in this mutant strain, restored full CAT activity. The level of CAT activity expressed from a T7 RNA polymerase promoter was not affected by the presence of either antisense RNA or the hammerhead ribozyme. When the target gene was expressed from a chromosomal locus in wild-type E. coli, both antisense RNA and the hammerhead ribozyme showed some inhibitory activity, but the level of inhibition was significantly increased in the slow ribosome strain. This bacterial system offers a unique entry to the study of cellular factors which mediate the activity of ribozymes in vivo.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Chen H,Ferbeyre G,Cedergren Rdoi
10.1038/nbt0597-432subject
Has Abstractpub_date
1997-05-01 00:00:00pages
432-5issue
5eissn
1087-0156issn
1546-1696journal_volume
15pub_type
杂志文章abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/80301
更新日期：2000-10-01 00:00:00
abstract：:The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contain...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0498-376
更新日期：1998-04-01 00:00:00
abstract：:The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1632
更新日期：2010-05-01 00:00:00
abstract：:Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1511
更新日期：2008-12-01 00:00:00
abstract：:Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/78504
更新日期：2000-08-01 00:00:00
abstract：:Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2890
更新日期：2014-05-01 00:00:00
abstract：:We describe a new method of random mutagenesis that employs the addition of peptide tails with random sequences to the C-terminal of enzyme molecules. A mutant population of catalase I from Bacillus stearothermophilus prepared by this method has a diversity in thermostability and enzyme activity equal to that obtained...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/5232
更新日期：1999-01-01 00:00:00
abstract：:Ensuring adequate manufacturing capacity for recombinant and monoclonal antibodies is one of the greatest challenges facing the biotech industry. The following factors should be taken into account when designing product development and production strategies. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0905-1054
更新日期：2005-09-01 00:00:00
abstract：:Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4201
更新日期：2018-10-01 00:00:00
abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1530
更新日期：2009-04-01 00:00:00
abstract：:Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0398-281
更新日期：1998-03-01 00:00:00
abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1404
更新日期：2008-05-01 00:00:00
abstract：:The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0208-183
更新日期：2008-02-01 00:00:00
abstract：:The nonprofit Pew Initiative on Food and Biotechnology is closing, but the need for an independent and neutral body to facilitate dialog on US biotech policy has never been greater. ...
journal_title：Nature biotechnology
pub_type： 社论
doi：10.1038/nbt0207-143
更新日期：2007-02-01 00:00:00
abstract：:Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1097-984
更新日期：1997-10-01 00:00:00
abstract：:Analyzing patent data from 1995-2005 show the increasing importance of patenting by small and medium-sized biotech companies. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0606-643
更新日期：2006-06-01 00:00:00
abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6146
更新日期：1999-02-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 已发布勘误
doi：10.1038/s41587-019-0167-2
更新日期：2019-07-01 00:00:00
abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0696-731
更新日期：1996-06-01 00:00:00
abstract：:The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1132
更新日期：2005-09-01 00:00:00
abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2798
更新日期：2014-02-01 00:00:00
abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3199
更新日期：2015-05-01 00:00:00
abstract：:Heterologous pathways used in metabolic engineering may produce intermediates toxic to the cell. Dynamic control of pathway enzymes could prevent the accumulation of these metabolites, but such a strategy requires sensors, which are largely unknown, that can detect and respond to the metabolite. Here we applied whole-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2689
更新日期：2013-11-01 00:00:00
abstract：:The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cu...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0996-1155
更新日期：1996-09-01 00:00:00
abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2862
更新日期：2014-05-01 00:00:00
abstract：:Complex interactions among genetic components often result in variable systemic performance in designed multigene systems. Using the bacterial clustered regularly interspaced short palindromic repeat (CRISPR) pathway we develop a synthetic RNA-processing platform, and show that efficient and specific cleavage of precu...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2355
更新日期：2012-10-01 00:00:00
abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.3765
更新日期：2017-03-01 00:00:00
abstract：:We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3886
更新日期：2017-07-01 00:00:00
abstract：:This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt828
更新日期：2003-06-01 00:00:00
abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/75390
更新日期：2000-05-01 00:00:00
