Abstract:
:An amendment to this paper has been published and can be accessed via a link at the top of the paper.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Ienca M,Haselager P,Emanuel EJdoi
10.1038/s41587-019-0167-2subject
Has Abstractpub_date
2019-07-01 00:00:00pages
819issue
7eissn
1087-0156issn
1546-1696pii
10.1038/s41587-019-0167-2journal_volume
37pub_type
已发布勘误abstract::Industrial strain development requires system-wide engineering and optimization of cellular metabolism while considering industrially relevant fermentation and recovery processes. It can be conceptualized as several strategies, which may be implemented in an iterative fashion and in different orders. The key challenge...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.3365
更新日期:2015-10-01 00:00:00
abstract::Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt986
更新日期:2004-07-01 00:00:00
abstract::Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4004
更新日期:2017-12-01 00:00:00
abstract::Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. Stage (S) 7 cells expressed key markers of mature pancreatic...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3033
更新日期:2014-11-01 00:00:00
abstract::Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1599
更新日期:2010-01-01 00:00:00
abstract::RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel nucleic acid-based therapeutic against a wide variety of diseases, including cancer, infectious diseases and genetic disorders. Antiviral RNAi strategies have r...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt1369
更新日期:2007-12-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1323
更新日期:2007-08-01 00:00:00
abstract::Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3585
更新日期:2016-08-01 00:00:00
abstract::The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0197-9
更新日期:2019-08-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-019-0292-y
更新日期:2019-11-01 00:00:00
abstract::The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/84422
更新日期:2001-02-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0457-8
更新日期:2020-03-01 00:00:00
abstract::Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1511
更新日期:2008-12-01 00:00:00
abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt853
更新日期:2003-09-01 00:00:00
abstract::The construction of synthetic cell-cell communication networks can improve our quantitative understanding of naturally occurring signaling pathways and enhance our capabilities to engineer coordinated cellular behavior in cell populations. Towards accomplishing these goals in eukaryotes, we developed and analyzed two ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1162
更新日期:2005-12-01 00:00:00
abstract::Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1404
更新日期:2008-05-01 00:00:00
abstract::The human transcriptome is marked by extensive alternative mRNA splicing and the expression of many closely related genes, which may be difficult to distinguish using standard microarray techniques. Here we describe a sensitive and specific assay for parallel analysis of mRNA isoforms on a fiber-optic microarray platf...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0402-353
更新日期:2002-04-01 00:00:00
abstract::The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1296-1675
更新日期:1996-12-01 00:00:00
abstract::The treatment of asthma and allergic rhinitis using unique, humanized anti-IgE monoclonal antibodies with very particular binding specificities is now supported by the results of multiple phase II and III human clinical studies. The therapeutic efficacy of this approach is attributable to several pharmacological mecha...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/72601
更新日期:2000-02-01 00:00:00
abstract::Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes. Current approaches for transcript reconstruction from such data often rely on aligning reads to a reference genome, and are thus unsuitable for samples with a partial or missing reference genome. Here we present the Trinity...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1883
更新日期:2011-05-15 00:00:00
abstract::Although mass spectrometry has become a powerful tool for the functional analysis of biological systems, complete proteome characterization cannot yet be achieved. Instead, the sheer complexity of living organisms demands fractionation of cellular extracts to enable more targeted analyses. Here, we introduce the conce...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1076
更新日期:2005-04-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0658-1
更新日期:2020-09-01 00:00:00
abstract::Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0333-6
更新日期:2019-12-01 00:00:00
abstract::Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0596-600
更新日期:1996-05-01 00:00:00
abstract::Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1216
更新日期:2006-06-01 00:00:00
abstract::We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/70761
更新日期:1999-12-01 00:00:00
abstract::Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0178-z
更新日期:2019-09-01 00:00:00
abstract::While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent of designer biocatalysts, produced by informed selection and mutation through recombinant DNA tec...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt0102-37
更新日期:2002-01-01 00:00:00
abstract::A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0897-759
更新日期:1997-08-01 00:00:00
abstract::We have evaluated inhibition of the plasmid-born chloramphenicol acetyl transferase gene (CAT) by the hammerhead ribozyme and antisense RNA in Escherichia coli where the translation and transcription rates have been modified. Whereas neither antisense nor the hammerhead had an inhibitory effect on CAT activity in wild...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0597-432
更新日期:1997-05-01 00:00:00