Abstract：
:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, human skin grafts show a reproducible pattern of progressive human T-cell infiltration and human graft microvascular injury that resembles human first-set skin graft rejection. Murine Mab to human LFA-3 or human LFA-3-IgG1 fusion protein, but not isotype-matched control antibodies, each markedly protected skin grafts from leukocyte infiltration and injury. These data provide the first evidence that LFA-3 functions in vivo and establish the ability of this new model to test human-specific immune modulators.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Sultan P,Schechner JS,McNiff JM,Hochman PS,Hughes CC,Lorber MI,Askenase PW,Pober JSdoi
10.1038/nbt0897-759subject
Has Abstractpub_date
1997-08-01 00:00:00pages
759-62issue
8eissn
1087-0156issn
1546-1696journal_volume
15pub_type
杂志文章abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1275
更新日期：2007-01-01 00:00:00
abstract：:Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3122
更新日期：2015-03-01 00:00:00
abstract：:Monitoring the T cell receptor (TCR) repertoire in health and disease can provide key insights into adaptive immune responses, but the accuracy of current TCR sequencing (TCRseq) methods is unclear. In this study, we systematically compared the results of nine commercial and academic TCRseq methods, including six rapi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0656-3
更新日期：2020-09-07 00:00:00
abstract：:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/11765
更新日期：1999-08-01 00:00:00
abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2798
更新日期：2014-02-01 00:00:00
abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1211
更新日期：2006-06-01 00:00:00
abstract：:We have identified a heptapeptide with high affinity to rheumatoid arthritis-associated class II major histocompatibility (MHC) molecules. Using a model of its interaction with the class II binding site, a variety of mimetic substitutions were introduced into the peptide. Several unnatural amino acids and dipeptide mi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/9865
更新日期：1999-06-01 00:00:00
abstract：:Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3865
更新日期：2017-07-01 00:00:00
abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0322-9
更新日期：2020-02-01 00:00:00
abstract：:Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0293-x
更新日期：2019-12-01 00:00:00
abstract：:Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0702-729
更新日期：2002-07-01 00:00:00
abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1259
更新日期：2006-11-01 00:00:00
abstract：:Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt726
更新日期：2002-09-01 00:00:00
abstract：:Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0602-575
更新日期：2002-06-01 00:00:00
abstract：:Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1009
更新日期：2004-09-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41587-020-0457-8
更新日期：2020-03-01 00:00:00
abstract：:Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0104-109
更新日期：2004-01-01 00:00:00
abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4047
更新日期：2018-02-01 00:00:00
abstract：:A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0628-7
更新日期：2021-01-01 00:00:00
abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3412
更新日期：2015-12-01 00:00:00
abstract：:By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0397-278
更新日期：1997-03-01 00:00:00
abstract：:Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a ce...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0202-149
更新日期：2002-02-01 00:00:00
abstract：:The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe....
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0502-473
更新日期：2002-05-01 00:00:00
abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2768
更新日期：2013-12-01 00:00:00
abstract：:Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1588
更新日期：2009-12-01 00:00:00
abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/80301
更新日期：2000-10-01 00:00:00
abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4052
更新日期：2018-02-01 00:00:00
abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0260-6
更新日期：2019-11-01 00:00:00
abstract：:The human transcriptome is marked by extensive alternative mRNA splicing and the expression of many closely related genes, which may be difficult to distinguish using standard microarray techniques. Here we describe a sensitive and specific assay for parallel analysis of mRNA isoforms on a fiber-optic microarray platf...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0402-353
更新日期：2002-04-01 00:00:00
abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0067-5
更新日期：2019-04-01 00:00:00
