Safeguarding CRISPR-Cas9 gene drives in yeast.

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:Protein phosphorylation is a dominant mechanism of information transfer in cells, and a major goal of current proteomic efforts is to generate a system-level map describing all the sites of protein phosphorylation. Recent efforts have focused on developing technologies for enriching and quantifying phosphopeptides. Id...

journal_title：Nature biotechnology

authors： Knight ZA,Schilling B,Row RH,Kenski DM,Gibson BW,Shokat KM

更新日期：2003-09-01 00:00:00

abstract：:RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and clinical research, but the determinants of targeting specificity and the extent of off-target cleavage remain insufficiently understood. Using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), we mapped genom...

journal_title：Nature biotechnology

authors： Kuscu C,Arslan S,Singh R,Thorpe J,Adli M

更新日期：2014-07-01 00:00:00

abstract：:Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...

journal_title：Nature biotechnology

authors： Sönnichsen C,Reinhard BM,Liphardt J,Alivisatos AP

更新日期：2005-06-01 00:00:00

abstract：:We have evaluated inhibition of the plasmid-born chloramphenicol acetyl transferase gene (CAT) by the hammerhead ribozyme and antisense RNA in Escherichia coli where the translation and transcription rates have been modified. Whereas neither antisense nor the hammerhead had an inhibitory effect on CAT activity in wild...

journal_title：Nature biotechnology

authors： Chen H,Ferbeyre G,Cedergren R

更新日期：1997-05-01 00:00:00

abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...

journal_title：Nature biotechnology

authors： Segal E,Sirlin CB,Ooi C,Adler AS,Gollub J,Chen X,Chan BK,Matcuk GR,Barry CT,Chang HY,Kuo MD

更新日期：2007-06-01 00:00:00

abstract：:Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogen...

journal_title：Nature biotechnology

authors： Li YH,Zhou G,Ma J,Jiang W,Jin LG,Zhang Z,Guo Y,Zhang J,Sui Y,Zheng L,Zhang SS,Zuo Q,Shi XH,Li YF,Zhang WK,Hu Y,Kong G,Hong HL,Tan B,Song J,Liu ZX,Wang Y,Ruan H,Yeung CK,Liu J,Wang H,Zhang LJ,Guan RX

更新日期：2014-10-01 00:00:00

abstract：:A detailed understanding of the function of neural networks and how they are supported by a dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we present confocal light field microscopy, a method that enables fast volumetric imaging in the brain at depths of hundreds of micrometers....

journal_title：Nature biotechnology

authors： Zhang Z,Bai L,Cong L,Yu P,Zhang T,Shi W,Li F,Du J,Wang K

更新日期：2021-01-01 00:00:00

abstract：:As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...

journal_title：Nature biotechnology

authors： Rabin S

更新日期：2006-05-01 00:00:00

abstract：:The basics of determining valuation will help you to understand what criteria matter most for investors. ...

journal_title：Nature biotechnology

authors： Frei P,Leleux B

更新日期：2004-08-01 00:00:00

abstract：:Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced ...

journal_title：Nature biotechnology

authors： Bar-Nur O,Verheul C,Sommer AG,Brumbaugh J,Schwarz BA,Lipchina I,Huebner AJ,Mostoslavsky G,Hochedlinger K

更新日期：2015-07-01 00:00:00

abstract：:We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...

journal_title：Nature biotechnology

authors： Marszalek PE,Li H,Fernandez JM

更新日期：2001-03-01 00:00:00

abstract：:To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine beta- and kappa-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resul...

journal_title：Nature biotechnology

authors： Brophy B,Smolenski G,Wheeler T,Wells D,L'Huillier P,Laible G

更新日期：2003-02-01 00:00:00

abstract：:Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...

journal_title：Nature biotechnology

authors： Superti-Furga G,Jönsson K,Courtneidge SA

更新日期：1996-05-01 00:00:00

abstract：:Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumo...

journal_title：Nature biotechnology

authors： Pulido J,Kottke T,Thompson J,Galivo F,Wongthida P,Diaz RM,Rommelfanger D,Ilett E,Pease L,Pandha H,Harrington K,Selby P,Melcher A,Vile R

更新日期：2012-03-18 00:00:00

abstract：:Engineered T cells are effective therapies against a range of malignancies, but current approaches rely on autologous T cells, which are difficult and expensive to manufacture. Efforts to develop potent allogeneic T cells that are not rejected by the recipient's immune system require abrogating both T- and natural kil...

journal_title：Nature biotechnology

authors： Mo F,Watanabe N,McKenna MK,Hicks MJ,Srinivasan M,Gomes-Silva D,Atilla E,Smith T,Ataca Atilla P,Ma R,Quach D,Heslop HE,Brenner MK,Mamonkin M

更新日期：2021-01-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce ...

journal_title：Nature biotechnology

authors： Ajjawi I,Verruto J,Aqui M,Soriaga LB,Coppersmith J,Kwok K,Peach L,Orchard E,Kalb R,Xu W,Carlson TJ,Francis K,Konigsfeld K,Bartalis J,Schultz A,Lambert W,Schwartz AS,Brown R,Moellering ER

更新日期：2017-07-01 00:00:00

abstract：:Neuronal precursor cells are widespread in the forebrain ventricular/subventricular zone, and may provide a cellular substrate for brain repair. Clonal lines derived from single progenitors can become progressively less representative of their parental precursors with time and passage in vitro. We have developed an al...

journal_title：Nature biotechnology

authors： Wang S,Wu H,Jiang J,Delohery TM,Isdell F,Goldman SA

更新日期：1998-02-01 00:00:00

abstract：:Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...

journal_title：Nature biotechnology

authors： Krusche P,Trigg L,Boutros PC,Mason CE,De La Vega FM,Moore BL,Gonzalez-Porta M,Eberle MA,Tezak Z,Lababidi S,Truty R,Asimenos G,Funke B,Fleharty M,Chapman BA,Salit M,Zook JM,Global Alliance for Genomics and Health Benchma

更新日期：2019-05-01 00:00:00

abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...

journal_title：Nature biotechnology

authors： Isaacs FJ,Dwyer DJ,Ding C,Pervouchine DD,Cantor CR,Collins JJ

更新日期：2004-07-01 00:00:00

abstract：:Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...

journal_title：Nature biotechnology

authors： Pollock R,Giel M,Linher K,Clackson T

更新日期：2002-07-01 00:00:00

abstract：:The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblas...

journal_title：Nature biotechnology

authors： Smith ZD,Nachman I,Regev A,Meissner A

更新日期：2010-05-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Simoni A,Hammond AM,Beaghton AK,Galizi R,Taxiarchi C,Kyrou K,Meacci D,Gribble M,Morselli G,Burt A,Nolan T,Crisanti A

更新日期：2020-09-01 00:00:00

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...

journal_title：Nature biotechnology

authors： Mallick P,Schirle M,Chen SS,Flory MR,Lee H,Martin D,Ranish J,Raught B,Schmitt R,Werner T,Kuster B,Aebersold R

更新日期：2007-01-01 00:00:00

abstract：:We describe a new method of random mutagenesis that employs the addition of peptide tails with random sequences to the C-terminal of enzyme molecules. A mutant population of catalase I from Bacillus stearothermophilus prepared by this method has a diversity in thermostability and enzyme activity equal to that obtained...

journal_title：Nature biotechnology

authors： Matsuura T,Miyai K,Trakulnaleamsai S,Yomo T,Shima Y,Miki S,Yamamoto K,Urabe I

更新日期：1999-01-01 00:00:00

abstract：:Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million to...

journal_title：Nature biotechnology

authors： López-Bucio J,de La Vega OM,Guevara-García A,Herrera-Estrella L

更新日期：2000-04-01 00:00:00

abstract：:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the...

journal_title：Nature biotechnology

authors： Kobayashi M,Shimizu S

更新日期：1998-08-01 00:00:00

abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...

journal_title：Nature biotechnology

authors： Kaplan N,Dekker J

更新日期：2013-12-01 00:00:00