Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming.

abstract：:Nitrogen-use efficiency of indica varieties of rice is superior to that of japonica varieties. We apply 16S ribosomal RNA gene profiling to characterize root microbiota of 68 indica and 27 japonica varieties grown in the field. We find that indica and japonica recruit distinct root microbiota. Notably, indica-enriched...

journal_title：Nature biotechnology

authors： Zhang J,Liu YX,Zhang N,Hu B,Jin T,Xu H,Qin Y,Yan P,Zhang X,Guo X,Hui J,Cao S,Wang X,Wang C,Wang H,Qu B,Fan G,Yuan L,Garrido-Oter R,Chu C,Bai Y

更新日期：2019-06-01 00:00:00

abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...

journal_title：Nature biotechnology

authors： Verkhusha VV,Lukyanov KA

更新日期：2004-03-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...

journal_title：Nature biotechnology

authors： Segal E,Sirlin CB,Ooi C,Adler AS,Gollub J,Chen X,Chan BK,Matcuk GR,Barry CT,Chang HY,Kuo MD

更新日期：2007-06-01 00:00:00

abstract：:Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from ...

journal_title：Nature biotechnology

authors： Richards M,Fong CY,Chan WK,Wong PC,Bongso A

更新日期：2002-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Ramsköld D,Luo S,Wang YC,Li R,Deng Q,Faridani OR,Daniels GA,Khrebtukova I,Loring JF,Laurent LC,Schroth GP,Sandberg R

更新日期：2020-03-01 00:00:00

abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...

journal_title：Nature biotechnology

authors： Mallick P,Schirle M,Chen SS,Flory MR,Lee H,Martin D,Ranish J,Raught B,Schmitt R,Werner T,Kuster B,Aebersold R

更新日期：2007-01-01 00:00:00

abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...

journal_title：Nature biotechnology

authors： Kalhor R,Tjong H,Jayathilaka N,Alber F,Chen L

更新日期：2011-12-25 00:00:00

abstract：:Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...

journal_title：Nature biotechnology

authors： Chick JM,Kolippakkam D,Nusinow DP,Zhai B,Rad R,Huttlin EL,Gygi SP

更新日期：2015-07-01 00:00:00

abstract：:N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...

journal_title：Nature biotechnology

authors： Wilson C,Chen PJ,Miao Z,Liu DR

更新日期：2020-12-01 00:00:00

abstract：:The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single ce...

journal_title：Nature biotechnology

authors： Lan F,Demaree B,Ahmed N,Abate AR

更新日期：2017-07-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

更新日期：2019-11-01 00:00:00

abstract：:A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...

journal_title：Nature biotechnology

authors： Narushima M,Kobayashi N,Okitsu T,Tanaka Y,Li SA,Chen Y,Miki A,Tanaka K,Nakaji S,Takei K,Gutierrez AS,Rivas-Carrillo JD,Navarro-Alvarez N,Jun HS,Westerman KA,Noguchi H,Lakey JR,Leboulch P,Tanaka N,Yoon JW

更新日期：2005-10-01 00:00:00

abstract：:A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient proces...

journal_title：Nature biotechnology

authors： Wittrup A,Ai A,Liu X,Hamar P,Trifonova R,Charisse K,Manoharan M,Kirchhausen T,Lieberman J

更新日期：2015-08-01 00:00:00

abstract：:Small-interfering RNAs (siRNAs) assemble into RISC, the RNA-induced silencing complex, which cleaves complementary mRNAs. Despite their fluctuating efficacy, siRNAs are widely used to assess gene function. Although this limitation could be ascribed, in part, to variations in the assembly and activation of RISC, downst...

journal_title：Nature biotechnology

authors： Tafer H,Ameres SL,Obernosterer G,Gebeshuber CA,Schroeder R,Martinez J,Hofacker IL

更新日期：2008-05-01 00:00:00

abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...

journal_title：Nature biotechnology

authors： Schöpke C,Taylor N,Cárcamo R,Konan NK,Marmey P,Henshaw GG,Beachy RN,Fauquet C

更新日期：1996-06-01 00:00:00

abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...

journal_title：Nature biotechnology

authors： Wang Z,Zhu T,Qiao C,Zhou L,Wang B,Zhang J,Chen C,Li J,Xiao X

更新日期：2005-03-01 00:00:00

abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

journal_title：Nature biotechnology

authors： Bzikadze AV,Pevzner PA

更新日期：2020-11-01 00:00:00

abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...

journal_title：Nature biotechnology

authors： Moore JC,Arnold FH

更新日期：1996-04-01 00:00:00

abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...

journal_title：Nature biotechnology

authors：

更新日期：2009-09-01 00:00:00

abstract：:We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...

journal_title：Nature biotechnology

authors： Mukherjee S,Seshadri R,Varghese NJ,Eloe-Fadrosh EA,Meier-Kolthoff JP,Göker M,Coates RC,Hadjithomas M,Pavlopoulos GA,Paez-Espino D,Yoshikuni Y,Visel A,Whitman WB,Garrity GM,Eisen JA,Hugenholtz P,Pati A,Ivanova NN,Woyke

更新日期：2017-07-01 00:00:00

abstract：:A major challenge for fluorescence imaging of living mammalian cells is maintaining viability following prolonged exposure to excitation illumination. We have monitored the dynamics of mitochondrial distribution in hamster embryos at frequent intervals over 24 h using two-photon microscopy (1,047 nm) while maintaining...

journal_title：Nature biotechnology

authors： Squirrell JM,Wokosin DL,White JG,Bavister BD

更新日期：1999-08-01 00:00:00

abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

journal_title：Nature biotechnology

authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

更新日期：2012-12-01 00:00:00

abstract：:Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...

journal_title：Nature biotechnology

authors： Eppich HM,Foxall R,Gaynor K,Dombkowski D,Miura N,Cheng T,Silva-Arrieta S,Evans RH,Mangano JA,Preffer FI,Scadden DT

更新日期：2000-08-01 00:00:00

abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...

journal_title：Nature biotechnology

authors： Nakai H,Storm TA,Kay MA

更新日期：2000-05-01 00:00:00

abstract：:The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...

journal_title：Nature biotechnology

authors： Latouche JB,Sadelain M

更新日期：2000-04-01 00:00:00

abstract：:Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and br...

journal_title：Nature biotechnology

authors： Lee D,Hyun JH,Jung K,Hannan P,Kwon HB

更新日期：2017-09-01 00:00:00

abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...

journal_title：Nature biotechnology

authors： Kroon E,Martinson LA,Kadoya K,Bang AG,Kelly OG,Eliazer S,Young H,Richardson M,Smart NG,Cunningham J,Agulnick AD,D'Amour KA,Carpenter MK,Baetge EE

更新日期：2008-04-01 00:00:00

abstract：:Genetic studies of human disease have traditionally focused on the detection of disease-causing mutations in afflicted individuals. Here we describe a complementary approach that seeks to identify healthy individuals resilient to highly penetrant forms of genetic childhood disorders. A comprehensive screen of 874 gene...

journal_title：Nature biotechnology

authors： Chen R,Shi L,Hakenberg J,Naughton B,Sklar P,Zhang J,Zhou H,Tian L,Prakash O,Lemire M,Sleiman P,Cheng WY,Chen W,Shah H,Shen Y,Fromer M,Omberg L,Deardorff MA,Zackai E,Bobe JR,Levin E,Hudson TJ,Groop L,Wang J,

更新日期：2016-05-01 00:00:00