Flexibility--the guiding principle for antibody manufacturing.

abstract：:Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...

journal_title：Nature biotechnology

authors： Buchholz F,Stewart AF

更新日期：2001-11-01 00:00:00

abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...

journal_title：Nature biotechnology

authors： Verkhusha VV,Lukyanov KA

更新日期：2004-03-01 00:00:00

abstract：:CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...

journal_title：Nature biotechnology

authors： Doench JG,Fusi N,Sullender M,Hegde M,Vaimberg EW,Donovan KF,Smith I,Tothova Z,Wilen C,Orchard R,Virgin HW,Listgarten J,Root DE

更新日期：2016-02-01 00:00:00

abstract：:Cells isolated from the embryonic, neonatal, and adult rodent central nervous system divide in response to epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2), while retaining the ability to differentiate into neurons and glia. These cultures can be grown in aggregates termed neurospheres, which conta...

journal_title：Nature biotechnology

authors： Caldwell MA,He X,Wilkie N,Pollack S,Marshall G,Wafford KA,Svendsen CN

更新日期：2001-05-01 00:00:00

abstract：:While the use of enzymes as biocatalysts to assist in the industrial manufacture of fine chemicals and pharmaceuticals has enormous potential, application is frequently limited by evolution-led catalyst traits. The advent of designer biocatalysts, produced by informed selection and mutation through recombinant DNA tec...

journal_title：Nature biotechnology

authors： Burton SG,Cowan DA,Woodley JM

更新日期：2002-01-01 00:00:00

abstract：:To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...

journal_title：Nature biotechnology

authors： Ikeno M,Grimes B,Okazaki T,Nakano M,Saitoh K,Hoshino H,McGill NI,Cooke H,Masumoto H

更新日期：1998-05-01 00:00:00

abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...

journal_title：Nature biotechnology

authors： Moore JC,Arnold FH

更新日期：1996-04-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,

更新日期：2020-08-01 00:00:00

abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...

journal_title：Nature biotechnology

authors： Cross KL,Campbell JH,Balachandran M,Campbell AG,Cooper SJ,Griffen A,Heaton M,Joshi S,Klingeman D,Leys E,Yang Z,Parks JM,Podar M

更新日期：2019-11-01 00:00:00

abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...

journal_title：Nature biotechnology

authors： Wang SJ

更新日期：2008-05-01 00:00:00

abstract：:Recent therapeutic successes have renewed interest in drug combinations, but experimental screening approaches are costly and often identify only small numbers of synergistic combinations. The DREAM consortium launched an open challenge to foster the development of in silico methods to computationally rank 91 compound...

journal_title：Nature biotechnology

authors： Bansal M,Yang J,Karan C,Menden MP,Costello JC,Tang H,Xiao G,Li Y,Allen J,Zhong R,Chen B,Kim M,Wang T,Heiser LM,Realubit R,Mattioli M,Alvarez MJ,Shen Y,NCI-DREAM Community.,Gallahan D,Singer D,Saez-Rodriguez J,

更新日期：2014-12-01 00:00:00

abstract：:Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs), it has historically been biased towards just a few. To measure the entire set of PTMs across diverse proteomes, software must overcome the dual challenges of covering enormous search spaces ...

journal_title：Nature biotechnology

authors： Devabhaktuni A,Lin S,Zhang L,Swaminathan K,Gonzalez CG,Olsson N,Pearlman SM,Rawson K,Elias JE

更新日期：2019-04-01 00:00:00

abstract：:Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common...

journal_title：Nature biotechnology

authors： Doxey AC,Yaish MW,Griffith M,McConkey BJ

更新日期：2006-07-01 00:00:00

abstract：:Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...

journal_title：Nature biotechnology

authors： Richins RD,Kaneva I,Mulchandani A,Chen W

更新日期：1997-10-01 00:00:00

abstract：:Protein phosphorylation is a dominant mechanism of information transfer in cells, and a major goal of current proteomic efforts is to generate a system-level map describing all the sites of protein phosphorylation. Recent efforts have focused on developing technologies for enriching and quantifying phosphopeptides. Id...

journal_title：Nature biotechnology

authors： Knight ZA,Schilling B,Row RH,Kenski DM,Gibson BW,Shokat KM

更新日期：2003-09-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...

journal_title：Nature biotechnology

authors： Marino M,Ruvo M,De Falco S,Fassina G

更新日期：2000-07-01 00:00:00

abstract：:Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a ce...

journal_title：Nature biotechnology

authors： Wang RF,Wang HY

更新日期：2002-02-01 00:00:00

abstract：:Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...

journal_title：Nature biotechnology

authors： O'Rourke KP,Loizou E,Livshits G,Schatoff EM,Baslan T,Manchado E,Simon J,Romesser PB,Leach B,Han T,Pauli C,Beltran H,Rubin MA,Dow LE,Lowe SW

更新日期：2017-06-01 00:00:00

abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...

journal_title：Nature biotechnology

authors： Li Y,Drummond DA,Sawayama AM,Snow CD,Bloom JD,Arnold FH

更新日期：2007-09-01 00:00:00

abstract：:Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylase base editors (GBEs) consist of a Cas9 nickase, a cytidine deaminase...

journal_title：Nature biotechnology

authors： Zhao D,Li J,Li S,Xin X,Hu M,Price MA,Rosser SJ,Bi C,Zhang X

更新日期：2021-01-01 00:00:00

abstract：:The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes compr...

journal_title：Nature biotechnology

authors： Berger I,Fitzgerald DJ,Richmond TJ

更新日期：2004-12-01 00:00:00

abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...

journal_title：Nature biotechnology

authors： Eisenstein M

更新日期：2011-02-01 00:00:00

abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...

journal_title：Nature biotechnology

authors： Hosaka T,Ohnishi-Kameyama M,Muramatsu H,Murakami K,Tsurumi Y,Kodani S,Yoshida M,Fujie A,Ochi K

更新日期：2009-05-01 00:00:00

abstract：:The vast selection of Drosophila mutants is an extraordinary resource for exploring molecular events underlying development and disease. We have designed and constructed an instrument that automatically separates Drosophila embryos of one genotype from a larger population of embryos, based on a fluorescent protein mar...

journal_title：Nature biotechnology

authors： Furlong EE,Profitt D,Scott MP

更新日期：2001-02-01 00:00:00

abstract：:The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...

journal_title：Nature biotechnology

authors： Zhu L,van de Lavoir MC,Albanese J,Beenhouwer DO,Cardarelli PM,Cuison S,Deng DF,Deshpande S,Diamond JH,Green L,Halk EL,Heyer BS,Kay RM,Kerchner A,Leighton PA,Mather CM,Morrison SL,Nikolov ZL,Passmore DB,Pradas-Monne

更新日期：2005-09-01 00:00:00

abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...

journal_title：Nature biotechnology

authors： Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama IN

更新日期：1997-01-01 00:00:00

abstract：:DNA microarrays have the ability to analyze the expression of thousands of the same set of genes under at least two different experimental conditions. However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial tota...

journal_title：Nature biotechnology

authors： Talaat AM,Hunter P,Johnston SA

更新日期：2000-06-01 00:00:00

abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...

journal_title：Nature biotechnology

authors： Ding J,Adiconis X,Simmons SK,Kowalczyk MS,Hession CC,Marjanovic ND,Hughes TK,Wadsworth MH,Burks T,Nguyen LT,Kwon JYH,Barak B,Ge W,Kedaigle AJ,Carroll S,Li S,Hacohen N,Rozenblatt-Rosen O,Shalek AK,Villani AC,Regev

更新日期：2020-06-01 00:00:00

abstract：:Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...

journal_title：Nature biotechnology

authors： Shimbo I,Nakajima R,Yokoyama S,Sumikura K

更新日期：2004-01-01 00:00:00

abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...

journal_title：Nature biotechnology

authors： Oberpenning F,Meng J,Yoo JJ,Atala A

更新日期：1999-02-01 00:00:00