Abstract：
:Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common motif, domain or fold among different AFPs. We have formulated a generic ice-binding model and incorporated it into a physicochemical pattern-recognition algorithm. It successfully recognizes ice-binding surfaces for a diverse range of AFPs, and clearly discriminates AFPs from other structures in the Protein Data Bank. The algorithm was used to identify a novel AFP from winter rye, and the antifreeze activity of this protein was subsequently confirmed. The presence of a common and distinct physicochemical pattern provides a structural basis for unifying AFPs from fish, insects and plants.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Doxey AC,Yaish MW,Griffith M,McConkey BJdoi
10.1038/nbt1224subject
Has Abstractpub_date
2006-07-01 00:00:00pages
852-5issue
7eissn
1087-0156issn
1546-1696pii
nbt1224journal_volume
24pub_type
杂志文章abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/7952
更新日期：1999-04-01 00:00:00
abstract：:The unique bread-making characteristic of wheat flour is closely related to the elasticity and extensibility of the gluten proteins stored in the starchy endosperm, particularly the high-molecular-weight glutenin subunits (HMW-GS), which are important in determining gluten and dough elasticity. The quality of wheat cu...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0996-1155
更新日期：1996-09-01 00:00:00
abstract：:Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3659
更新日期：2016-01-01 00:00:00
abstract：:Microbially catalyzed reactions, which occur in the natural sulfur cycle, have been integrated in a microbiological process to remove toxic metals from contaminated soils. Bioleaching using sulfuric acid produced by sulfur-oxidizing bacteria was followed by precipitation of the leachate metals as insoluble sulfides by...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0698-572
更新日期：1998-06-01 00:00:00
abstract：:One-size-fits-all intravenous delivery of biologics may be giving way to alternate delivery routes that enable safer and more efficient drug administration. Michael Eisenstein reports. ...
journal_title：Nature biotechnology
pub_type： 新闻
doi：10.1038/nbt.1768
更新日期：2011-02-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. T...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt792
更新日期：2003-03-01 00:00:00
abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/72671
更新日期：2000-02-01 00:00:00
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journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/6146
更新日期：1999-02-01 00:00:00
abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/75390
更新日期：2000-05-01 00:00:00
abstract：:Rice is a staple crop that has undergone substantial phenotypic and physiological changes during domestication. Here we resequenced the genomes of 40 cultivated accessions selected from the major groups of rice and 10 accessions of their wild progenitors (Oryza rufipogon and Oryza nivara) to >15 × raw data coverage. W...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2011-12-11 00:00:00
abstract：:The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1296-1675
更新日期：1996-12-01 00:00:00
abstract：:Recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the riboso...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt986
更新日期：2004-07-01 00:00:00
abstract：:Identification of the peptides recognized by individual T cells is important for understanding and treating immune-related diseases. Current cytometry-based approaches are limited to the simultaneous screening of 10-100 distinct T-cell specificities in one sample. Here we use peptide-major histocompatibility complex (...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3662
更新日期：2016-10-01 00:00:00
abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3353
更新日期：2015-10-01 00:00:00
abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4047
更新日期：2018-02-01 00:00:00
abstract：:Most microbes in the biosphere remain unculturable. Whole genome shotgun (WGS) sequencing of environmental DNA (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. However, in communities of high complexity, metagenomics fails to link specific microbes to specific ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1488
更新日期：2008-09-01 00:00:00
abstract：:Influenza A virus leads to yearly epidemics and sporadic pandemics. Present prophylactic strategies focus on egg-grown, live, attenuated influenza vaccines (LAIVs), in which attenuation is generated by conferring temperature sensitivity onto the virus. Here we describe an alternative approach to attenuating influenza ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1542
更新日期：2009-06-01 00:00:00
abstract：:Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1097-984
更新日期：1997-10-01 00:00:00
abstract：:The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1197-1271
更新日期：1997-11-01 00:00:00
abstract：:Efficient analysis of very large amounts of raw data for peptide identification and protein quantification is a principal challenge in mass spectrometry (MS)-based proteomics. Here we describe MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data. Using correlatio...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1511
更新日期：2008-12-01 00:00:00
abstract：:Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1-4. Here we introduce a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorit...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0293-x
更新日期：2019-12-01 00:00:00
abstract：:Protein phosphorylation is a dominant mechanism of information transfer in cells, and a major goal of current proteomic efforts is to generate a system-level map describing all the sites of protein phosphorylation. Recent efforts have focused on developing technologies for enriching and quantifying phosphopeptides. Id...
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更新日期：2003-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/nbt0905-1054
更新日期：2005-09-01 00:00:00
abstract：:Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strai...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
abstract：:Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0997-859
更新日期：1997-09-01 00:00:00
abstract：:Although bile duct disorders are well-recognized causes of liver disease, the molecular and cellular events leading to biliary dysfunction are poorly understood. To enable modeling and drug discovery for biliary disease, we describe a protocol that achieves efficient differentiation of biliary epithelial cells (cholan...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3294
更新日期：2015-08-01 00:00:00
abstract：:High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-inter...
journal_title：Nature biotechnology
pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
abstract：:Analysis of abnormally methylated genes is increasingly important in basic research and in the development of cancer biomarkers. We have developed methyl-BEAMing technology to enable absolute quantification of the number of methylated molecules in a sample. Individual DNA fragments are amplified and analyzed either by...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1559
更新日期：2009-09-01 00:00:00
abstract：:A Pennsylvania company hopes to turn synthetic DNA vaccines into rapid response agents against flu epidemics and cancer. ...
journal_title：Nature biotechnology
pub_type： 新闻
doi：10.1038/nbt0213-98
更新日期：2013-02-01 00:00:00
