Abstract：
:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplastomic cultivars. Primers specific to cpDNA were used to demonstrate maternal inheritance of chloroplasts in 47 natural hybrids between cultivated B. napus and wild B. rapa. We conclude that there will be no or negligible pollen-mediated chloroplast dispersal from oilseed rape. Transgene introgression could still occur in mixed populations, however, if B. napus acted as the recurrent female parent. Rate of transfer would then depend on the abundance of mixed populations, their persistence as mixtures, and hybridization frequency within stands. A low incidence of sympatry (0.6-0.7%) between wild B. rapa and cultivated B. napus along the river Thames, UK, in 1997 and 1998, suggests mixed stands will form only rarely. Eighteen feral populations of B. napus also showed a strong tendency toward rapid decline in plant number, seed return, and ultimately, extinction within 3 years. Conversely, hybrid production is significant in mixed stands, and the absence of control practices means that oilseed rape will have slightly greater persistence. We infer that some introgression from transplastomic B. napus into B. rapa is inevitable in mixed populations even though such populations will occur infrequently and will tend to lose B. napus plants relatively quickly. Chloroplast exchange will be extremely rare and scattered.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Scott SE,Wilkinson MJdoi
10.1038/7952subject
Has Abstractpub_date
1999-04-01 00:00:00pages
390-2issue
4eissn
1087-0156issn
1546-1696journal_volume
17pub_type
杂志文章abstract：:We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1077
更新日期：2005-04-01 00:00:00
abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2768
更新日期：2013-12-01 00:00:00
abstract：:N6-Methyladenosine (m6A) is the most widespread internal messenger RNA modification in humans. Despite recent progress in understanding the biological roles of m6A, the inability to install m6A site specifically in individual transcripts has hampered efforts to elucidate causal relationships between the presence of a ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0572-6
更新日期：2020-12-01 00:00:00
abstract：:The use of DNA variants in the mapping of the human genome and in the positional cloning of monogenic disease genes is well established. Determining the genetic bases of the more common "multifactorial" diseases, however, presents a major challenge. The genetics of these diseases are complicated by the interplay betwe...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0198-33
更新日期：1998-01-01 00:00:00
abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0496-458
更新日期：1996-04-01 00:00:00
abstract：:Replicate mass spectrometry (MS) measurements and the use of multiple analytical methods can greatly expand the comprehensiveness of shotgun proteomic profiling of biological samples. However, the inherent biases and variations in such data create computational and statistical challenges for quantitative comparative a...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.1592
更新日期：2010-01-01 00:00:00
abstract：:The exocrine pancreas, liver, and submandibular glands of the rat were used to express and secrete two exogenous, human protein hormones (growth hormone and insulin) into blood at physiological concentrations. Transfection, expression, and secretion were achieved by the in vivo retrograde injection of plasmid DNA into...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1297-1378
更新日期：1997-12-01 00:00:00
abstract：:Current tools for targeted RNA editing rely on the delivery of exogenous proteins or chemically modified guide RNAs, which may lead to aberrant effector activity, delivery barrier or immunogenicity. Here, we present an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs s...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0178-z
更新日期：2019-09-01 00:00:00
abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0344-3
更新日期：2020-03-01 00:00:00
abstract：:An analysis of US Federal Circuit decisions shows strategies for designing around patents. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0508-519
更新日期：2008-05-01 00:00:00
abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4260
更新日期：2018-10-29 00:00:00
abstract：:Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strai...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2858
更新日期：2014-04-01 00:00:00
abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0797-663
更新日期：1997-07-01 00:00:00
abstract：:Little is known about the consequences of releasing genetically engineered plants (GEP) into the environment. Using opine-producing GEP, we show that transgenic plants alter their biological environment, more precisely the root-associated bacterial populations. The alterations were both transgene-specific and target p...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0497-369
更新日期：1997-04-01 00:00:00
abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/72671
更新日期：2000-02-01 00:00:00
abstract：:Three national patent offices have consulted on patents that cover protein three-dimensional structural data and pharmacophores, with significant implications for the biotechnology industry. ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt0104-109
更新日期：2004-01-01 00:00:00
abstract：:The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines into a therapeutic HER2/neu antibody at three sites differing in solvent accessibility and local charge. The highly solvent-accessible si...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2108
更新日期：2012-01-22 00:00:00
abstract：:Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt0207-185
更新日期：2007-02-01 00:00:00
abstract：:Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2519
更新日期：2013-04-01 00:00:00
abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...
journal_title：Nature biotechnology
pub_type： 杂志文章,评审
doi：10.1038/nbt.4022
更新日期：2017-12-01 00:00:00
abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.3412
更新日期：2015-12-01 00:00:00
abstract：:Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/11765
更新日期：1999-08-01 00:00:00
abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-020-0465-8
更新日期：2020-06-01 00:00:00
abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.2862
更新日期：2014-05-01 00:00:00
abstract：:Pluripotent stem cell-derived cardiomyocyte grafts can remuscularize substantial amounts of infarcted myocardium and beat in synchrony with the heart, but in some settings cause ventricular arrhythmias. It is unknown whether human cardiomyocytes can restore cardiac function in a physiologically relevant large animal m...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt.4162
更新日期：2018-08-01 00:00:00
abstract：:Mass spectrometry-based quantitative proteomics has become an important component of biological and clinical research. Although such analyses typically assume that a protein's peptide fragments are observed with equal likelihood, only a few so-called 'proteotypic' peptides are repeatedly and consistently identified fo...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt1275
更新日期：2007-01-01 00:00:00
abstract：:This report describes the selection of highly efficient antibody catalysts by combining chemical selection from a synthetic library with directed in vitro protein evolution. Evolution started from a naive antibody library displayed on phage made from fully synthetic, antibody-encoding genes (the Human Combinatorial An...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/nbt828
更新日期：2003-06-01 00:00:00
abstract：:A variety of strategies to incorporate unnatural amino acids into proteins have been pursued, but all have limitations with respect to technical accessibility, scalability, applicability to in vivo studies, or site specificity of amino acid incorporation. The ability to selectively introduce unnatural functional group...
journal_title：Nature biotechnology
pub_type：
doi：10.1038/nbt742
更新日期：2002-10-01 00:00:00
abstract：:We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluores...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/81192
更新日期：2000-11-01 00:00:00
abstract：:Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...
journal_title：Nature biotechnology
pub_type： 杂志文章
doi：10.1038/s41587-019-0392-8
更新日期：2020-03-01 00:00:00
