Genetically engineered plants producing opines alter their biological environment.

abstract：:L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...

journal_title：Nature biotechnology

authors： Agius F,González-Lamothe R,Caballero JL,Muñoz-Blanco J,Botella MA,Valpuesta V

更新日期：2003-02-01 00:00:00

abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...

journal_title：Nature biotechnology

authors： Tibbe AG,de Grooth BG,Greve J,Liberti PA,Dolan GJ,Terstappen LW

更新日期：1999-12-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Ramsköld D,Luo S,Wang YC,Li R,Deng Q,Faridani OR,Daniels GA,Khrebtukova I,Loring JF,Laurent LC,Schroth GP,Sandberg R

更新日期：2020-03-01 00:00:00

abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...

journal_title：Nature biotechnology

authors： Cross KL,Campbell JH,Balachandran M,Campbell AG,Cooper SJ,Griffen A,Heaton M,Joshi S,Klingeman D,Leys E,Yang Z,Parks JM,Podar M

更新日期：2019-11-01 00:00:00

abstract：:Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...

journal_title：Nature biotechnology

authors： Faria M,Spiller DG,Dubertret C,Nelson JS,White MR,Scherman D,Hélène C,Giovannangeli C

更新日期：2001-01-01 00:00:00

abstract：:DNA delivery of tumor antigens can activate specific immune attack on cancer cells. However, antigens may be weak, and immune capacity can be compromised. Fusion of genes encoding activating sequences to the tumor antigen sequence facilitates promotion and manipulation of effector pathways. Idiotypic determinants of B...

journal_title：Nature biotechnology

authors： Savelyeva N,Munday R,Spellerberg MB,Lomonossoff GP,Stevenson FK

更新日期：2001-08-01 00:00:00

abstract：:A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...

journal_title：Nature biotechnology

authors： Sultan P,Schechner JS,McNiff JM,Hochman PS,Hughes CC,Lorber MI,Askenase PW,Pober JS

更新日期：1997-08-01 00:00:00

abstract：:Relatively low levels of expression from naturally occurring promoters have limited the use of muscle as a gene therapy target. Myogenic restricted gene promoters display complex organization usually involving combinations of several myogenic regulatory elements. By random assembly of E-box, MEF-2, TEF-1, and SRE site...

journal_title：Nature biotechnology

authors： Li X,Eastman EM,Schwartz RJ,Draghia-Akli R

更新日期：1999-03-01 00:00:00

abstract：:Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, ...

journal_title：Nature biotechnology

authors： Yuasa S,Itabashi Y,Koshimizu U,Tanaka T,Sugimura K,Kinoshita M,Hattori F,Fukami S,Shimazaki T,Ogawa S,Okano H,Fukuda K

更新日期：2005-05-01 00:00:00

abstract：:Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced ...

journal_title：Nature biotechnology

authors： Bar-Nur O,Verheul C,Sommer AG,Brumbaugh J,Schwarz BA,Lipchina I,Huebner AJ,Mostoslavsky G,Hochedlinger K

更新日期：2015-07-01 00:00:00

abstract：:To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...

journal_title：Nature biotechnology

authors： Ikeno M,Grimes B,Okazaki T,Nakano M,Saitoh K,Hoshino H,McGill NI,Cooke H,Masumoto H

更新日期：1998-05-01 00:00:00

abstract：:Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...

journal_title：Nature biotechnology

authors： Chen W,Zhao Y,Chen X,Yang Z,Xu X,Bi Y,Chen V,Li J,Choi H,Ernest B,Tran B,Mehta M,Kumar P,Farmer A,Mir A,Mehra UA,Li JL,Moos M Jr,Xiao W,Wang C

更新日期：2020-12-21 00:00:00

abstract：:Numerous biological processes involve the recognition of a specific pattern of binding sites on a target protein or surface. Although ligands displayed by disordered scaffolds form stochastic rather than specific patterns, theoretical models predict that recognition will occur between patterns that are characterized b...

journal_title：Nature biotechnology

authors： Rai P,Padala C,Poon V,Saraph A,Basha S,Kate S,Tao K,Mogridge J,Kane RS

更新日期：2006-05-01 00:00:00

abstract：:Industrial strain development requires system-wide engineering and optimization of cellular metabolism while considering industrially relevant fermentation and recovery processes. It can be conceptualized as several strategies, which may be implemented in an iterative fashion and in different orders. The key challenge...

journal_title：Nature biotechnology

authors： Lee SY,Kim HU

更新日期：2015-10-01 00:00:00

abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...

journal_title：Nature biotechnology

authors： Nakai H,Storm TA,Kay MA

更新日期：2000-05-01 00:00:00

abstract：:A Pennsylvania company hopes to turn synthetic DNA vaccines into rapid response agents against flu epidemics and cancer. ...

journal_title：Nature biotechnology

authors： Rohn J

更新日期：2013-02-01 00:00:00

abstract：:Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...

journal_title：Nature biotechnology

authors： Palukaitis P,Roossinck MJ

更新日期：1996-10-01 00:00:00

abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...

journal_title：Nature biotechnology

authors： D'Amour KA,Bang AG,Eliazer S,Kelly OG,Agulnick AD,Smart NG,Moorman MA,Kroon E,Carpenter MK,Baetge EE

更新日期：2006-11-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNA-seq) enables the systematic identification of cell populations in a tissue, but characterizing their spatial organization remains challenging. We combine a microarray-based spatial transcriptomics method that reveals spatial patterns of gene expression using an array of spots, each ca...

journal_title：Nature biotechnology

authors： Moncada R,Barkley D,Wagner F,Chiodin M,Devlin JC,Baron M,Hajdu CH,Simeone DM,Yanai I

更新日期：2020-03-01 00:00:00

abstract：:Epigenetic modifications constitute a complex regulatory layer on top of the genome sequence. Pluripotent and differentiated cells provide a powerful system for investigating how the epigenetic code influences cellular fate. High-throughput sequencing of these cell types has yielded DNA methylation maps at single-nucl...

journal_title：Nature biotechnology

authors： Meissner A

更新日期：2010-10-01 00:00:00

abstract：:Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and ...

journal_title：Nature biotechnology

authors： Barrangou R,Doudna JA

更新日期：2016-01-01 00:00:00

abstract：:The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...

journal_title：Nature biotechnology

authors： Lockhart DJ,Dong H,Byrne MC,Follettie MT,Gallo MV,Chee MS,Mittmann M,Wang C,Kobayashi M,Horton H,Brown EL

更新日期：1996-12-01 00:00:00

abstract：:Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...

journal_title：Nature biotechnology

authors： Zhang G,Liu X,Quan Z,Cheng S,Xu X,Pan S,Xie M,Zeng P,Yue Z,Wang W,Tao Y,Bian C,Han C,Xia Q,Peng X,Cao R,Yang X,Zhan D,Hu J,Zhang Y,Li H,Li H,Li N,Wang J,Wang C,Wang R,Guo T,Cai Y,Liu C,Xiang H,

更新日期：2012-05-13 00:00:00

abstract：:Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by far most of the crossovers occur between exons, rather than inside. The...

journal_title：Nature biotechnology

authors： Kolkman JA,Stemmer WP

更新日期：2001-05-01 00:00:00

abstract：:The interaction between the HSP90 chaperone and its client kinases is sensitive to the conformational status of the kinase, and stabilization of the kinase fold by small molecules strongly decreases chaperone interaction. Here we exploit this observation and assay small-molecule binding to kinases in living cells, usi...

journal_title：Nature biotechnology

authors： Taipale M,Krykbaeva I,Whitesell L,Santagata S,Zhang J,Liu Q,Gray NS,Lindquist S

更新日期：2013-07-01 00:00:00

abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...

journal_title：Nature biotechnology

authors： Schulman KA,Tunis SR

更新日期：2010-11-01 00:00:00

abstract：:Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...

journal_title：Nature biotechnology

authors： O'Rourke KP,Loizou E,Livshits G,Schatoff EM,Baslan T,Manchado E,Simon J,Romesser PB,Leach B,Han T,Pauli C,Beltran H,Rubin MA,Dow LE,Lowe SW

更新日期：2017-06-01 00:00:00

abstract：:Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...

journal_title：Nature biotechnology

authors： Buchholz F,Stewart AF

更新日期：2001-11-01 00:00:00

abstract：:The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single ce...

journal_title：Nature biotechnology

authors： Lan F,Demaree B,Ahmed N,Abate AR

更新日期：2017-07-01 00:00:00

abstract：:Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes. Current approaches for transcript reconstruction from such data often rely on aligning reads to a reference genome, and are thus unsuitable for samples with a partial or missing reference genome. Here we present the Trinity...

journal_title：Nature biotechnology

authors： Grabherr MG,Haas BJ,Yassour M,Levin JZ,Thompson DA,Amit I,Adiconis X,Fan L,Raychowdhury R,Zeng Q,Chen Z,Mauceli E,Hacohen N,Gnirke A,Rhind N,di Palma F,Birren BW,Nusbaum C,Lindblad-Toh K,Friedman N,Regev A

更新日期：2011-05-15 00:00:00