当前位置： SCI文献检索 > NATURE BIOTECHNOLOGY期刊下所有文献 > Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage.

Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage.

Abstract：

:Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced pluripotent stem cells (iPSCs) and induced neural stem cells (iNSCs) under conditions previously shown to induce only iNSCs. Fibroblast-derived iNSC colonies silenced retroviral transgenes and reactivated silenced X chromosomes, both hallmarks of pluripotent stem cells. Moreover, lineage tracing with an Oct4-CreER labeling system demonstrated that virtually all iNSC colonies originated from cells transiently expressing Oct4, whereas ablation of Oct4(+) cells prevented iNSC formation. Lastly, an alternative transdifferentiation cocktail that lacks Oct4 and was reportedly unable to support induced pluripotency yielded iPSCs and iNSCs carrying the Oct4-CreER-derived lineage label. Together, these data suggest that iNSC generation from fibroblasts using OKSM and other pluripotency-related reprogramming factors requires passage through a transient iPSC state.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Bar-Nur O,Verheul C,Sommer AG,Brumbaugh J,Schwarz BA,Lipchina I,Huebner AJ,Mostoslavsky G,Hochedlinger K

doi

10.1038/nbt.3247

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

761-8

issue

7

eissn

1087-0156

issn

1546-1696

pii

nbt.3247

journal_volume

33

pub_type

杂志文章

相关文献

NATURE BIOTECHNOLOGY文献大全
  • Low probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapa.

    abstract：:Pollen-mediated movement of transgenes from transplastomic oilseed rape (Brassica napus) into wild relatives will be avoided if chloroplasts are maternally transmitted. We assess the probability of chloroplast exchange between conventional oilseed rape and wild Brassica rapa to model the future behavior of transplasto...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/7952

    authors： Scott SE,Wilkinson MJ

    更新日期：1999-04-01 00:00:00

  • Alteration of Cre recombinase site specificity by substrate-linked protein evolution.

    abstract：:Directed molecular evolution was applied to generate Cre recombinase variants that recognize a new DNA target sequence. Cre was adapted in a three-stage strategy to evolve recombinases to specifically recombine the new site. This complex multicycle task was made feasible by an improved directed-evolution procedure tha...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1101-1047

    authors： Buchholz F,Stewart AF

    更新日期：2001-11-01 00:00:00

  • Transgenic mice with defined combinations of drug-inducible reprogramming factors.

    abstract：:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.1520

    authors： Markoulaki S,Hanna J,Beard C,Carey BW,Cheng AW,Lengner CJ,Dausman JA,Fu D,Gao Q,Wu S,Cassady JP,Jaenisch R

    更新日期：2009-02-01 00:00:00

  • High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays.

    abstract：:Methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current technologies have limited sensitivity or throughput. Here we present an approach to precisely and rapidly measure growth rates of many individual cells simultaneously. We flow cells in suspen...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.3666

    authors： Cermak N,Olcum S,Delgado FF,Wasserman SC,Payer KR,A Murakami M,Knudsen SM,Kimmerling RJ,Stevens MM,Kikuchi Y,Sandikci A,Ogawa M,Agache V,Baléras F,Weinstock DM,Manalis SR

    更新日期：2016-10-01 00:00:00

  • De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits.

    abstract：:Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogen...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2979

    authors： Li YH,Zhou G,Ma J,Jiang W,Jin LG,Zhang Z,Guo Y,Zhang J,Sui Y,Zheng L,Zhang SS,Zuo Q,Shi XH,Li YF,Zhang WK,Hu Y,Kong G,Hong HL,Tan B,Song J,Liu ZX,Wang Y,Ruan H,Yeung CK,Liu J,Wang H,Zhang LJ,Guan RX

    更新日期：2014-10-01 00:00:00

  • Automated assembly of centromeres from ultra-long error-prone reads.

    abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/s41587-020-0582-4

    authors： Bzikadze AV,Pevzner PA

    更新日期：2020-11-01 00:00:00

  • Preventing gene silencing with human replicators.

    abstract：:Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators). When replicators were included in si...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1202

    authors： Fu H,Wang L,Lin CM,Singhania S,Bouhassira EE,Aladjem MI

    更新日期：2006-05-01 00:00:00

  • Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.

    abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1259

    authors： D'Amour KA,Bang AG,Eliazer S,Kelly OG,Agulnick AD,Smart NG,Moorman MA,Kroon E,Carpenter MK,Baetge EE

    更新日期：2006-11-01 00:00:00

  • Mutation detection by electrocatalysis at DNA-modified electrodes.

    abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/80301

    authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

    更新日期：2000-10-01 00:00:00

  • RNAi modulation of placental sFLT1 for the treatment of preeclampsia.

    abstract：:Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.4297

    authors： Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

    更新日期：2018-11-19 00:00:00

  • Author Correction: Brain leaks and consumer neurotechnology.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature biotechnology

    pub_type： 已发布勘误

    doi：10.1038/s41587-019-0167-2

    authors： Ienca M,Haselager P,Emanuel EJ

    更新日期：2019-07-01 00:00:00

  • Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays.

    abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/76469

    authors： Brenner S,Johnson M,Bridgham J,Golda G,Lloyd DH,Johnson D,Luo S,McCurdy S,Foy M,Ewan M,Roth R,George D,Eletr S,Albrecht G,Vermaas E,Williams SR,Moon K,Burcham T,Pallas M,DuBridge RB,Kirchner J,Fearon K,Mao J,

    更新日期：2000-06-01 00:00:00

  • Identification of co-regulated genes through Bayesian clustering of predicted regulatory binding sites.

    abstract：:The identification of co-regulated genes and their transcription-factor binding sites (TFBS) are key steps toward understanding transcription regulation. In addition to effective laboratory assays, various computational approaches for the detection of TFBS in promoter regions of coexpressed genes have been developed. ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt802

    authors： Qin ZS,McCue LA,Thompson W,Mayerhofer L,Lawrence CE,Liu JS

    更新日期：2003-04-01 00:00:00

  • Tumor refractoriness to anti-VEGF treatment is mediated by CD11b+Gr1+ myeloid cells.

    abstract：:Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1323

    authors： Shojaei F,Wu X,Malik AK,Zhong C,Baldwin ME,Schanz S,Fuh G,Gerber HP,Ferrara N

    更新日期：2007-08-01 00:00:00

  • Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

    abstract：:Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq ena...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/s41587-018-0007-9

    authors： Arora S,Steuernagel B,Gaurav K,Chandramohan S,Long Y,Matny O,Johnson R,Enk J,Periyannan S,Singh N,Asyraf Md Hatta M,Athiyannan N,Cheema J,Yu G,Kangara N,Ghosh S,Szabo LJ,Poland J,Bariana H,Jones JDG,Bentley AR,A

    更新日期：2019-02-01 00:00:00

  • DNA patenting: the end of an era?

    abstract：:Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章,评审

    doi：10.1038/nbt0207-185

    authors： Hopkins MM,Mahdi S,Patel P,Thomas SM

    更新日期：2007-02-01 00:00:00

  • Synthetic CD4 exocyclics inhibit binding of human immunodeficiency virus type 1 envelope to CD4 and virus replication in T lymphocytes.

    abstract：:CD4 functions as a major T-cell surface receptor for human immunodeficiency virus by binding the human immunodeficiency virus type 1 (HIV-1) envelope protein gp120 with relatively high affinity. We have developed constrained aromatically modified analogs of the secondary structures of the first domain of CD4 in order ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt0297-150

    authors： Zhang X,Gaubin M,Briant L,Srikantan V,Murali R,Saragovi U,Weiner D,Devaux C,Autiero M,Piatier-Tonneau D,Greene MI

    更新日期：1997-02-01 00:00:00

  • Optogenetic control of endogenous Ca(2+) channels in vivo.

    abstract：:Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.3350

    authors： Kyung T,Lee S,Kim JE,Cho T,Park H,Jeong YM,Kim D,Shin A,Kim S,Baek J,Kim J,Kim NY,Woo D,Chae S,Kim CH,Shin HS,Han YM,Kim D,Heo WD

    更新日期：2015-10-01 00:00:00

  • The paths around stem cell intellectual property.

    abstract：:Can new approaches for deriving human stem cells circumnavigate existing patents that dominate embryonic stem cell intellectual property? ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt0406-411

    authors： Taymor KS,Scott CT,Greely HT

    更新日期：2006-04-01 00:00:00

  • Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology.

    abstract：:Nitrile hydratase (NHase), which catalyzes the hydration of nitriles to amides, has been used in the industrial production of acrylamide and nicotinamide. Recent studies on NHases, which are roughly classified into iron and cobalt types according to the metal involved, have clarified the photoactivation mechanism, the...

    journal_title：Nature biotechnology

    pub_type： 杂志文章,评审

    doi：10.1038/nbt0898-733

    authors： Kobayashi M,Shimizu S

    更新日期：1998-08-01 00:00:00

  • Reversible permeabilization of plasma membranes with an engineered switchable pore.

    abstract：:By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt0397-278

    authors： Russo MJ,Bayley H,Toner M

    更新日期：1997-03-01 00:00:00

  • Microbial synthesis of medium-chain chemicals from renewables.

    abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

    journal_title：Nature biotechnology

    pub_type： 杂志文章,评审

    doi：10.1038/nbt.4022

    authors： Sarria S,Kruyer NS,Peralta-Yahya P

    更新日期：2017-12-01 00:00:00

  • Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms.

    abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.2862

    authors： Patro R,Mount SM,Kingsford C

    更新日期：2014-05-01 00:00:00

  • Publisher Correction: Continuous evolution of base editors with expanded target compatibility and improved activity.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature biotechnology

    pub_type： 已发布勘误

    doi：10.1038/s41587-019-0253-5

    authors： Thuronyi BW,Koblan LW,Levy JM,Yeh WH,Zheng C,Newby GA,Wilson C,Bhaumik M,Shubina-Oleinik O,Holt JR,Liu DR

    更新日期：2019-09-01 00:00:00

  • Multiplexed droplet single-cell RNA-sequencing using natural genetic variation.

    abstract：:Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.4042

    authors： Kang HM,Subramaniam M,Targ S,Nguyen M,Maliskova L,McCarthy E,Wan E,Wong S,Byrnes L,Lanata CM,Gate RE,Mostafavi S,Marson A,Zaitlen N,Criswell LA,Ye CJ

    更新日期：2018-01-01 00:00:00

  • Expression monitoring by hybridization to high-density oligonucleotide arrays.

    abstract：:The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1296-1675

    authors： Lockhart DJ,Dong H,Byrne MC,Follettie MT,Gallo MV,Chee MS,Mittmann M,Wang C,Kobayashi M,Horton H,Brown EL

    更新日期：1996-12-01 00:00:00

  • A generic protein purification method for protein complex characterization and proteome exploration.

    abstract：:We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, acti...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/13732

    authors： Rigaut G,Shevchenko A,Rutz B,Wilm M,Mann M,Séraphin B

    更新日期：1999-10-01 00:00:00

  • Generation of higher affinity T cell receptors by antigen-driven differentiation of progenitor T cells in vitro.

    abstract：:Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt.4004

    authors： Schmitt TM,Aggen DH,Ishida-Tsubota K,Ochsenreither S,Kranz DM,Greenberg PD

    更新日期：2017-12-01 00:00:00

  • Design of stable biologically active recombinant lutropin analogs.

    abstract：:Glycoprotein hormones are noncovalent heterodimers comprised of a common alpha subunit and a hormone-specific beta subunit. Secretion and biologic action of these hormones are dependent on the formation of the heterodimer. The human LH beta subunit is unique among the other beta subunits in that it assembles inefficie...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt0797-663

    authors： Garcia-Campayo V,Sato A,Hirsch B,Sugahara T,Muyan M,Hsueh AJ,Boime I

    更新日期：1997-07-01 00:00:00

  • A human beta-cell line for transplantation therapy to control type 1 diabetes.

    abstract：:A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telo...

    journal_title：Nature biotechnology

    pub_type： 杂志文章

    doi：10.1038/nbt1145

    authors： Narushima M,Kobayashi N,Okitsu T,Tanaka Y,Li SA,Chen Y,Miki A,Tanaka K,Nakaji S,Takei K,Gutierrez AS,Rivas-Carrillo JD,Navarro-Alvarez N,Jun HS,Westerman KA,Noguchi H,Lakey JR,Leboulch P,Tanaka N,Yoon JW

    更新日期：2005-10-01 00:00:00