Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

abstract：:Through sequential generations of random mutagenesis and screening, we have directed the evolution of an esterase for deprotection of an antibiotic p-nitrobenzyl ester in aqueous-organic solvents. Because rapid screening directly on the desired antibiotic (loracarbef) nucleus p-nitrobenzyl ester was not feasible, the ...

journal_title：Nature biotechnology

authors： Moore JC,Arnold FH

更新日期：1996-04-01 00:00:00

abstract：:A fibrinolytic agent consisting of a tissue-type plasminogen activator (tPA) coupled to the surface of red blood cells (RBCs) can dissolve nascent clots from within the clot, in a Trojan horse-like strategy, while having minimal effects on preexisting hemostatic clots or extravascular tissue. After intravenous injecti...

journal_title：Nature biotechnology

authors： Murciano JC,Medinilla S,Eslin D,Atochina E,Cines DB,Muzykantov VR

更新日期：2003-08-01 00:00:00

abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...

journal_title：Nature biotechnology

authors： Jung W,Yu O,Lau SM,O'Keefe DP,Odell J,Fader G,McGonigle B

更新日期：2000-02-01 00:00:00

abstract：:Comparing diverse single-cell RNA sequencing (scRNA-seq) datasets generated by different technologies and in different laboratories remains a major challenge. Here we address the need for guidance in choosing algorithms leading to accurate biological interpretations of varied data types acquired with different platfor...

journal_title：Nature biotechnology

authors： Chen W,Zhao Y,Chen X,Yang Z,Xu X,Bi Y,Chen V,Li J,Choi H,Ernest B,Tran B,Mehta M,Kumar P,Farmer A,Mir A,Mehra UA,Li JL,Moos M Jr,Xiao W,Wang C

更新日期：2020-12-21 00:00:00

abstract：:We describe a novel approach to design a set of primers selective for large groups of genes. This method is based on the distribution frequency of all nucleotide combinations (octa- to decanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect genes. By analyzing 1000 human ...

journal_title：Nature biotechnology

authors： López-Nieto CE,Nigam SK

更新日期：1996-07-01 00:00:00

abstract：:Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...

journal_title：Nature biotechnology

authors： Kyung T,Lee S,Kim JE,Cho T,Park H,Jeong YM,Kim D,Shin A,Kim S,Baek J,Kim J,Kim NY,Woo D,Chae S,Kim CH,Shin HS,Han YM,Kim D,Heo WD

更新日期：2015-10-01 00:00:00

abstract：:New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields. Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of ...

journal_title：Nature biotechnology

authors： Sakamoto T,Morinaka Y,Ohnishi T,Sunohara H,Fujioka S,Ueguchi-Tanaka M,Mizutani M,Sakata K,Takatsuto S,Yoshida S,Tanaka H,Kitano H,Matsuoka M

更新日期：2006-01-01 00:00:00

abstract：:Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor ...

journal_title：Nature biotechnology

authors： Markoulaki S,Hanna J,Beard C,Carey BW,Cheng AW,Lengner CJ,Dausman JA,Fu D,Gao Q,Wu S,Cassady JP,Jaenisch R

更新日期：2009-02-01 00:00:00

abstract：:Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows fo...

journal_title：Nature biotechnology

authors： Wolf-Levy H,Javitt A,Eisenberg-Lerner A,Kacen A,Ulman A,Sheban D,Dassa B,Fishbain-Yoskovitz V,Carmona-Rivera C,Kramer MP,Nudel N,Regev I,Zahavi L,Elinger D,Kaplan MJ,Morgenstern D,Levin Y,Merbl Y

更新日期：2018-10-22 00:00:00

abstract：:In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of...

journal_title：Nature biotechnology

authors： Meissner A,Wernig M,Jaenisch R

更新日期：2007-10-01 00:00:00

abstract：:Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for single-molecule bisulfite sequencing for digital quantification of DNA methylation at single-nucleotide...

journal_title：Nature biotechnology

authors： Deng J,Shoemaker R,Xie B,Gore A,LeProust EM,Antosiewicz-Bourget J,Egli D,Maherali N,Park IH,Yu J,Daley GQ,Eggan K,Hochedlinger K,Thomson J,Wang W,Gao Y,Zhang K

更新日期：2009-04-01 00:00:00

abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

journal_title：Nature biotechnology

authors： Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

更新日期：2018-02-01 00:00:00

abstract：:The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...

journal_title：Nature biotechnology

authors： Miller SM,Wang T,Randolph PB,Arbab M,Shen MW,Huang TP,Matuszek Z,Newby GA,Rees HA,Liu DR

更新日期：2020-04-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,

更新日期：2020-08-01 00:00:00

abstract：:We report a protocol for the rapid identification of DNA-binding proteins. Immobilized DNA probes harboring a specific sequence motif are incubated with cell or nuclear extract. Proteins are analyzed directly off the solid support by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The det...

journal_title：Nature biotechnology

authors： Nordhoff E,Krogsdam AM,Jorgensen HF,Kallipolitis BH,Clark BF,Roepstorff P,Kristiansen K

更新日期：1999-09-01 00:00:00

abstract：:Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...

journal_title：Nature biotechnology

authors： Cheung AS,Zhang DKY,Koshy ST,Mooney DJ

更新日期：2018-02-01 00:00:00

abstract：:Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that...

journal_title：Nature biotechnology

authors： Kang HM,Subramaniam M,Targ S,Nguyen M,Maliskova L,McCarthy E,Wan E,Wong S,Byrnes L,Lanata CM,Gate RE,Mostafavi S,Marson A,Zaitlen N,Criswell LA,Ye CJ

更新日期：2018-01-01 00:00:00

abstract：:A sequence-specific genomic delivery system for the correction of chromosomal mutations was designed by incorporating two different binding domains into a single-stranded oligonucleotide. A repair domain (RD) contained the native sequence of the target region. A third strand-forming domain (TFD) was designed to form a...

journal_title：Nature biotechnology

authors： Culver KW,Hsieh WT,Huyen Y,Chen V,Liu J,Khripine Y,Khorlin A

更新日期：1999-10-01 00:00:00

abstract：:Complex interactions among genetic components often result in variable systemic performance in designed multigene systems. Using the bacterial clustered regularly interspaced short palindromic repeat (CRISPR) pathway we develop a synthetic RNA-processing platform, and show that efficient and specific cleavage of precu...

journal_title：Nature biotechnology

authors： Qi L,Haurwitz RE,Shao W,Doudna JA,Arkin AP

更新日期：2012-10-01 00:00:00

abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...

journal_title：Nature biotechnology

authors： Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

更新日期：2007-04-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencin...

journal_title：Nature biotechnology

authors： Prensner JR,Iyer MK,Balbin OA,Dhanasekaran SM,Cao Q,Brenner JC,Laxman B,Asangani IA,Grasso CS,Kominsky HD,Cao X,Jing X,Wang X,Siddiqui J,Wei JT,Robinson D,Iyer HK,Palanisamy N,Maher CA,Chinnaiyan AM

更新日期：2011-07-31 00:00:00

abstract：:Despite developments in targeted gene sequencing and whole-genome analysis techniques, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Here we present targeted locus amplification (TLA), a strategy to se...

journal_title：Nature biotechnology

authors： de Vree PJ,de Wit E,Yilmaz M,van de Heijning M,Klous P,Verstegen MJ,Wan Y,Teunissen H,Krijger PH,Geeven G,Eijk PP,Sie D,Ylstra B,Hulsman LO,van Dooren MF,van Zutven LJ,van den Ouweland A,Verbeek S,van Dijk KW,Cornel

更新日期：2014-10-01 00:00:00

abstract：:The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...

journal_title：Nature biotechnology

authors： Latouche JB,Sadelain M

更新日期：2000-04-01 00:00:00

abstract：:Detection of mutations and damaged DNA bases is important for the early diagnosis of genetic disease. Here we describe an electrocatalytic method for the detection of single-base mismatches as well as DNA base lesions in fully hybridized duplexes, based on charge transport through DNA films. Gold electrodes modified w...

journal_title：Nature biotechnology

authors： Boon EM,Ceres DM,Drummond TG,Hill MG,Barton JK

更新日期：2000-10-01 00:00:00

abstract：:Tyrosine phosphorylation exerts a pivotal role in cell regulation processes of higher eukaryotes. Tight control of the activity of protein tyrosine kinases is crucial for ordered phosphorylation to occur. We have developed a functional screen for tyrosine kinase regulators using c-Src, the first cellular protein tyros...

journal_title：Nature biotechnology

authors： Superti-Furga G,Jönsson K,Courtneidge SA

更新日期：1996-05-01 00:00:00

abstract：:The nonprofit Pew Initiative on Food and Biotechnology is closing, but the need for an independent and neutral body to facilitate dialog on US biotech policy has never been greater. ...

journal_title：Nature biotechnology

authors：

更新日期：2007-02-01 00:00:00

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:Identification of the peptides recognized by individual T cells is important for understanding and treating immune-related diseases. Current cytometry-based approaches are limited to the simultaneous screening of 10-100 distinct T-cell specificities in one sample. Here we use peptide-major histocompatibility complex (...

journal_title：Nature biotechnology

authors： Bentzen AK,Marquard AM,Lyngaa R,Saini SK,Ramskov S,Donia M,Such L,Furness AJ,McGranahan N,Rosenthal R,Straten PT,Szallasi Z,Svane IM,Swanton C,Quezada SA,Jakobsen SN,Eklund AC,Hadrup SR

更新日期：2016-10-01 00:00:00

abstract：:Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...

journal_title：Nature biotechnology

authors： Pollock R,Giel M,Linher K,Clackson T

更新日期：2002-07-01 00:00:00